Molecular insight into regulation of miRNAs in the spleen of zebrafish (Danio rerio) upon pathogenic Streptococcus parauberis infection

Liyanage, T.D.; Nikapitiya, Chamilani; Lee, Jehee; Zoysa, Mahanama De

doi:10.1016/j.fsi.2020.08.045

Cited by 18 publications

(13 citation statements)

References 54 publications

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“…Regarding miR-146b in humans, it plays a role in the innate immune response 85 and is involved in gliomas and ovarian cancers 86 . In fish, very little data is available but it was upregulated in response to infection in zebrafish embryos 87 and spleen 88 and the sperm of growth hormone (GH)-transgenic zebrafish 79 . Overall, to our knowledge, this is the first time that the cellular localization of these two miRNAs are described in the gonads.…”

Section: Discussionmentioning

confidence: 99%

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2022

Sci Rep

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In the last decade, a plethora of microRNAs (miRNAs) has been reported in a wide variety of physiological processes, including reproduction, in many aquatic organisms. However, miRNAome alterations occurred by environmental cues due to water temperature increment have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, the animal model zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation, a treatment that results in male-skewed sex ratios in the adulthood. Once the fish reached adulthood, gonads were sequenced by high-throughput technologies and a total of 23 and 1 differentially expressed miRNAs in ovaries and testes, respectively, were identified two months after the heat treatment. Most of these heat-recorder miRNAs were involved in human sex-related cancer and about 400 predicted-target genes were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of the predicted target genes, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected heat-recorder miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events.

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Section: Discussionmentioning

confidence: 99%

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2022

Sci Rep

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“…Briefly, the collected tissues were processed, embedded, and sectioned to a thickness of 4 µm. The sectioned samples were placed on glass slides and stained with hematoxylin and eosin (H&E), according to the method described by Liyanage et al [54]. The slides were observed under a light microscope (Leica 3000 LED, Wetzlar, Germany).…”

Section: Cytotoxicity Of Octominin Against Murine Macrophage Cellsmentioning

confidence: 99%

Antimicrobial and Anti-Biofilm Peptide Octominin for Controlling Multidrug-Resistant Acinetobacter baumannii

Jayathilaka

Rajapaksha

Nikapitiya

et al. 2021

IJMS

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Acinetobacter baumannii is a serious nosocomial pathogen with multiple drug resistance (MDR), the control of which has become challenging due to the currently used antibiotics. Our main objective in this study is to determine the antibacterial and antibiofilm activities of the antimicrobial peptide, Octominin, against MDR A. baumannii and derive its possible modes of actions. Octominin showed significant bactericidal effects at a low minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of 5 and 10 µg/mL, respectively. Time-kill kinetic analysis and bacterial viability tests revealed that Octominin showed a concentration-dependent antibacterial activity. Field-emission scanning electron microscopy (FE-SEM) analysis revealed that Octominin treatment altered the morphology and membrane structure of A. baumannii. Propidium iodide (PI) and reactive oxygen species (ROS) generation assays showed that Octominin increased the membrane permeability and ROS generation in A. baumannii, thereby causing bacterial cell death. Further, a lipopolysaccharides (LPS) binding assay showed an Octominin concentration-dependent LPS neutralization ability. Biofilm formation inhibition and eradication assays further revealed that Octominin inhibited biofilm formation and showed a high biofilm eradication activity against A. baumannii. Furthermore, up to a concentration of 100 µg/mL, Octominin caused no hemolysis and cell viability changes in mammalian cells. An in vivo study in zebrafish showed that the Octominin-treated group had a significantly higher relative percentage survival (54.1%) than the untreated group (16.6%). Additionally, a reduced bacterial load and fewer alterations in histological analysis confirmed the successful control of A. baumannii by Octominin in vivo. Collectively, these data suggest that Octominin exhibits significant antibacterial and antibiofilm activities against the multidrug-resistant A. baumannii, and this AMP can be developed further as a potent AMP for the control of antibiotic resistance.

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“…Regarding miR-146b in humans, it plays a role in the innate immune response [100] and is involved in gliomas and ovarian cancers [101,102]. In fish, very little data is available but it was upregulated in response to infection in zebrafish embryos [103] and spleen [104] and the sperm of growth hormone (GH)-transgenic zebrafish [87]. Overall, to our knowledge, this is the first time that the cellular localization of these two miRNAs are described in the gonads.…”

Section: Discussionmentioning

confidence: 91%

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Gelderen

Montfort

Álvarez‐Dios

et al. 2021

Preprint

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MicroRNAs (miRNAs) are important post-transcriptional regulators of gene expression in a wide variety of physiological processes, including those related to the reproductive system. Although in the last decade a plethora of miRNAs has been reported, the miRNA alterations occurred by environmental cues and their biological functions have not yet been elucidated. With the aim to identify epigenetic regulations mediated by miRNAs in the gonads in a climate change scenario, zebrafish (Danio rerio) were subjected to high temperatures during sex differentiation (18-32 days post fertilization, dpf), a treatment that results in male-skewed sex ratios. Once the fish reached adulthood (90 dpf), ovaries and testes were sequenced by high-throughput technologies. About 101 million high-quality reads were obtained from gonadal samples. Analyses of the expression levels of the miRNAs identified a total of 23 and 1 differentially expressed (DE) miRNAs in ovaries and testes, respectively, two months after the heat treatment. Most of the identified miRNAs were involved in human sex-related cancer. After retrieving 3’ UTR regions, ~400 predicted targets of the 24 DE miRNAs were obtained, some with reproduction-related functions. Their synteny in the zebrafish genome was, for more than half of them, in the chromosomes 7, 2, 4, 3 and 11 in the ovaries, chromosome 4 being the place where the predicted sex-associated-region (sar) is localized in wild zebrafish. Further, spatial localization in the gonads of two selected miRNAs (miR-122-5p and miR-146-5p) showed exclusive expression in the ovarian germ cells. The present study expands the catalog of sex-specific miRNAs and deciphers, for the first time, thermosensitive miRNAs in the zebrafish gonads that might be used as potential epimarkers to predict environmental past events.

show abstract

Molecular insight into regulation of miRNAs in the spleen of zebrafish (Danio rerio) upon pathogenic Streptococcus parauberis infection

Cited by 18 publications

References 54 publications

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

Antimicrobial and Anti-Biofilm Peptide Octominin for Controlling Multidrug-Resistant Acinetobacter baumannii

Deciphering sex-specific miRNAs as heat-recorders in zebrafish

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