Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Brown‐Peterson, Nancy J.; Larkin, Patrick; Denslow, Nancy D.; King, Christina M.; Manning, Steve; Brouwer, Marius

doi:10.3354/meps286203

Cited by 43 publications

(22 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, increases in blue crab Hcy concentration during exposure to hypoxia did not result from increased protein synthesis, but was due to reduced protein degradation (Brown-Peterson et al 2005). Blue crabs down-regulate many genes, including those that code for Hcy, to conserve energy during hypoxic stress (Brown-Peterson et al 2005). Since there can be signiWcant physiological costs associated with increased Hcy concentration, the positive correlation between Hcy concentration and hypoxic exposure may not be adaptive, but merely the byproduct of another acclimatization response-energy conservation via reduced protein synthesis and degradation.…”

Section: Hcy Concentration Versus Hcy Structurementioning

confidence: 91%

“…Increased Hcy concentrations could also raise the viscosity of hemolymph, thereby increasing the energetic cost of circulating hemolymph. Moreover, increases in blue crab Hcy concentration during exposure to hypoxia did not result from increased protein synthesis, but was due to reduced protein degradation (Brown-Peterson et al 2005). Blue crabs down-regulate many genes, including those that code for Hcy, to conserve energy during hypoxic stress (Brown-Peterson et al 2005).…”

Section: Hcy Concentration Versus Hcy Structurementioning

confidence: 99%

See 1 more Smart Citation

Molecular keys unlock the mysteries of variable survival responses of blue crabs to hypoxia

2009

View full text Add to dashboard Cite

Hypoxia is a major stressor in coastal ecosystems, yet generalizing its impacts on fish and shellfish populations across hypoxic events is difficult due to variability among individuals in their history of exposure to hypoxia and related abiotic variables, and subsequent behavioral and survival responses. Although aquatic animals have diverse physiological responses to cope with hypoxia, we know little about how inter-individual variation in physiological state affects survival and behavioral decisions under hypoxic conditions. Laboratory experiments coupled with molecular techniques determined how extrinsic factors (e.g., water body and temperature) and respiratory physiology (hemocyanin concentration and structure) affected survival and behavior of adult blue crabs (Callinectes sapidus) exposed to different levels of hypoxia over a 30-h time period. Nearly 100% of crabs survived the 1.3 mg dissolved oxygen (DO) l(-1) treatment (18.4% air saturation), suggesting that adult blue crabs are tolerant of severe hypoxia. Probability of survival decreased with increasing hypoxic exposure time, lower DO, and increasing temperature. Individual-level differences in survival correlated with water body and crab size. Crabs collected from the oligo/mesohaline and hypoxic Neuse River Estuary (NRE), North Carolina, USA survived hypoxic exposures longer than crabs from the euhaline and normoxic Bogue and Back Sounds, North Carolina. Furthermore, small NRE crabs survived longer than large NRE crabs. Hemocyanin (Hcy) concentration did not explain these individual-level differences, however, hypoxia-tolerant crabs had Hcy structures indicative of a high-O(2)-affinity form of Hcy, suggesting Hcy "quality" (i.e., structure) may be more important for hypoxia survival than Hcy "quantity" (i.e., concentration). The geographic differences in survival we observed also highlight the importance of carefully selecting experimental animals when planning to extrapolate results to the population level.

show abstract

Section: Hcy Concentration Versus Hcy Structurementioning

confidence: 91%

Section: Hcy Concentration Versus Hcy Structurementioning

confidence: 99%

Molecular keys unlock the mysteries of variable survival responses of blue crabs to hypoxia

2009

View full text Add to dashboard Cite

show abstract

“…Furthermore, exposure to hypoxia can have profound physiological consequences on fish and invertebrates, such as alterations in systemic and molecular control mechanisms of oxygen transport (Cochran and Burnett 1996;Mangum 1997;Terwilliger 1998;McMahon 2001;Paul et al 2004). Recently, differential gene expression in fishes and crustaceans has been shown to reflect the metabolic roles of tissues during hypoxia exposure (Gracey et al 2001;Ton et al 2003;David et al 2005;van der Meer et al 2005;Brouwer et al 2005Brouwer et al , 2007Brown-Peterson et al 2005. However, all documented changes in gene expression to date have been conducted under controlled laboratory conditions, and the applicability of these responses to animals experiencing hypoxia in the wild remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Impacts of cyclic hypoxia on reproductive and gene expression patterns in the grass shrimp: field versus laboratory comparison

et al. 2010

View full text Add to dashboard Cite

Identification of organisms able to act as biological indicators of hypoxia exposure is important given the increasing frequency of hypoxic events worldwide. Grass shrimp, Palaemonetes pugio, are ubiquitous estuarine residents in the southeastern US, where they commonly experience cyclic hypoxia. We hypothesized that reproductive and gene expression patterns of grass shrimp in cyclic hypoxic field sites relative to normoxic sites would be similar to previous results from cyclic hypoxia laboratory experiments showing differential up-and downregulation of hypoxia-responsive genes. There were few differences in gene expression of indigenous shrimp collected during summer from two Gulf of Mexico estuarine systems [East Bay, FL (EB) and Weeks Bay, AL (WB)], although down-regulation of the gene coding for vitellogenin-1 corresponded with a decreased relative fecundity and fewer ovigerous females at cyclic hypoxic field sites, suggesting an overall impact on reproduction. The gene expression profiles of grass shrimp caged for 7 days in field sites differed by estuary, but few hypoxia-responsive genes identified in laboratory studies were differentially expressed in field shrimp. Overall, genes coding for protein synthesis, protein degradation, carbohydrate and lipid metabolism and electron transport were mostly up-regulated in EB caged shrimp but generally down-regulated in WB caged shrimp and laboratory shrimp. Thus, caged grass shrimp from different bay systems exhibited profoundly different gene expression profiles. Such profiles may serve as sensitive bioindicators of differences in water quality, habitat quality or food resources among estuaries but are not effective as indicators of hypoxia exposure.

show abstract

“…All studies underlined the quick modification of subunit patterns. Both, C. sapidus and C. marinus, show a significant variation in subunit pattern starting from 1-4 days after the experiment stimulus, revealed by changes of both protein expression (Mason et al, 1983;Spicer and Hodgson, 2003a,b) and gene activation (Brouwer et al, 2004;Brown-Peterson et al, 2005).…”

Section: Relative Variation: Change In Subunit Compositionmentioning

confidence: 99%

The molecular heterogeneity of hemocyanin: Its role in the adaptive plasticity of Crustacea

Giomi

Beltramini

2007

Gene

View full text Add to dashboard Cite

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Cited by 43 publications

References 52 publications

Molecular keys unlock the mysteries of variable survival responses of blue crabs to hypoxia

Molecular keys unlock the mysteries of variable survival responses of blue crabs to hypoxia

Impacts of cyclic hypoxia on reproductive and gene expression patterns in the grass shrimp: field versus laboratory comparison

The molecular heterogeneity of hemocyanin: Its role in the adaptive plasticity of Crustacea

Contact Info

Product

Resources

About