Molecular Imaging with 68Ga Radio-Nanomaterials: Shedding Light on Nanoparticles

Fernández‐Barahona, Irene; Muñoz-Hernando, María; Pellico, Juan; Ruíz-Cabello, Jesús; Herranz, Fernando

doi:10.3390/app8071098

Cited by 18 publications

(16 citation statements)

References 159 publications

(140 reference statements)

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“…Several approaches were already established for the development of novel dual PET/MRI contrast agents by coupling MRI contrast agents, e.g., Fe 3 O 4 nanoparticles, liposomes, or gadolinium, with different radioactive isotopes, e.g., fluorine-18 ( 18 F), galium-68, copper-64, iodine-124, or zirconium-89, to allow their visualization with both PET and MRI [27][28][29][30][31][32]. Compared to other commonly used PET isotopes, the use of 18 F is preferred as it is easy and cheap in production and leads to high resolution PET images due to abundant low-energy positron emission [33].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous in vivo PET/MRI using fluorine-18 labeled Fe3O4@Al(OH)3 nanoparticles: comparison of nanoparticle and nanoparticle-labeled stem cell distribution

et al. 2020

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Background: Mesenchymal stem cells (MSCs) have shown potential for treatment of different diseases. However, their working mechanism is still unknown. To elucidate this, the non-invasive and longitudinal tracking of MSCs would be beneficial. Both iron oxide-based nanoparticles (Fe 3 O 4 NPs) for magnetic resonance imaging (MRI) and radiotracers for positron emission tomography (PET) have shown potential as in vivo cell imaging agents. However, they are limited by their negative contrast and lack of spatial information as well as short half-life, respectively. In this proof-of-principle study, we evaluated the potential of Fe 3 O 4 @Al(OH) 3 NPs as dual PET/MRI contrast agents, as they allow stable binding of [ 18 F]F − ions to the NPs and thus, NP visualization and quantification with both imaging modalities. Results: 18 F-labeled Fe 3 O 4 @Al(OH) 3 NPs (radiolabeled NPs) or mouse MSCs (mMSCs) labeled with these radiolabeled NPs were intravenously injected in healthy C57Bl/6 mice, and their biodistribution was studied using simultaneous PET/ MRI acquisition. While liver uptake of radiolabeled NPs was seen with both PET and MRI, mMSCs uptake in the lungs could only be observed with PET. Even some initial loss of fluoride label did not impair NPs/mMSCs visualization. Furthermore, no negative effects on blood cell populations were seen after injection of either the NPs or mMSCs, indicating good biocompatibility. Conclusion: We present the application of novel 18 F-labeled Fe 3 O 4 @Al(OH) 3 NPs as safe cell tracking agents for simultaneous PET/MRI. Combining both modalities allows fast and easy NP and mMSC localization and quantification using PET at early time points, while MRI provides high-resolution, anatomic background information and long-term NP follow-up, hereby overcoming limitations of the individual imaging modalities.

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Section: Introductionmentioning

confidence: 99%

Simultaneous in vivo PET/MRI using fluorine-18 labeled Fe3O4@Al(OH)3 nanoparticles: comparison of nanoparticle and nanoparticle-labeled stem cell distribution

et al. 2020

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“…Germanium-68 has a T 1/2 = 270.95 ± 0.26 days 4 , and this permits the manufacture of potentially inexpensive and long-lived generator systems 5 . This is an advantage over usual PET radionuclides produced via proton irradiation such as 18 F, 11 C, 13 N, 15 O, 64 Cu and 124 I. An additional benefit of using 68 Ga is that it can be separated easily on demand and its availability in highly-specific activity, no-carrier-added form 6,7 .…”

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confidence: 99%

“…Another emerging generator is Galli Eo (from IRE EliT, Fleurus, Belgium), however, the column contains an unspecified resin. The generator is eluted with 0.1 M HCl, and has more than 67% elution yield and less than 1 × 10 -3 % 68 Ge breakthrough 18 . According to its brochure, metal content per elution is less than 1 ppm and ≤ 10 µg/GBq of 68 Ga for Fe, Cu, Ni, Zn, Pb and Al.…”

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confidence: 99%

“…The breakthrough of 68 Ge in the eluate is negligibly low (< 10 -4 %) and remains unchanged over the period of 1 year. Cu, Fe, Ce, Al, and Mn ion levels are always < 0.1 ppm [13][14][15][16][17][18][19][20] . This group has recently reported the clinical experience of 32 patients who were injected with [ 68 Ga]-Ga-DOTA-NOC prepared using 68 Ga eluted from the CeO 2 -PAN sorbent-based 68 Ge/ 68 Ga generator 21 .…”

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confidence: 99%

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Development and long-term evaluation of a new 68Ge/68Ga generator based on nano-SnO2 for PET imaging

Romero¹,

Martínez²,

Oteo³

et al. 2020

Sci Rep

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Radionuclide generator systems can routinely provide radionuclides on demand such as 68 Ga produced by a 68 Ge/ 68 Ga generator without the availability of an on-site accelerator or a research reactor. thus, in this work nano-Sno 2 was used to develop a new 68 Ge/ 68 Ga generator which was evaluated over a period of 17 months and 305 elution cycles. The elution yield was 91.1 ± 1.8% in the first 7 mL (1 M HCl as eluent) when the generator was new and then it decreased with time and use to 73.8 ± 1.9%. Around 80% of the elutable 68 Ga activity was obtained in 1 mL and the 68 Ge content in the eluate did not exceed 1 × 10-4 % over the investigation period when it was eluted regularly. The described generator provided adequate results for radiolabelling of DotA-toc with direct use of eluate. in addition, [ 68 Ga] Ga-DotA-toc was tested satisfactorily for in vivo tumor detection by micropet/ct imaging in a lung cancer mouse model.

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“…The T1 enhancement of FH can be attributed to several possible factors, such as increased surface iron core exposure (due to high surface area to volume ratio), 35 the surface effects on magnetization and water exchange [36][37][38] and suppressed magnetization at lower Fe concentration. 39 Thus, FH has relatively high r 1 (ie 10 À 20mM À1 :s À1 ) and r 2 (ie r 2 ¼ 57 À 60mM À1 :s À1 ) values 40 and can act as a simultaneous MR contrast agent.…”

Section: Phantom Imagingmentioning

confidence: 99%

<p>A Radio-Nano-Platform for T1/T2 Dual-Mode PET-MR Imaging</p>

Gholami

Yuan

Wilks

et al. 2020

IJN

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Video abstractPoint your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: http://youtu.be/Me_QBfX7I3sPurpose: This study aimed to develop a chelate-free radiolabeled nanoparticle platform for simultaneous positron emission tomography (PET) and magnetic resonance (MR) imaging that provides contrast-enhanced diagnostic imaging and significant image quality gain by integrating the high spatial resolution of MR with the high sensitivity of PET. Methods: A commercially available super-paramagnetic iron oxide nanoparticle (SPION) (Feraheme ® , FH) was labeled with the [ 89 Zr]Zr using a novel chelate-free radiolabeling technique, heat-induced radiolabeling (HIR). Radiochemical yield (RCY) and purity (RCP) were measured using size exclusion chromatography (SEC) and radio-thin layer chromatography (radio-TLC). Characterization of the non-radioactive isotope 90 Zr-labeled FH was performed by transmission electron microscopy (TEM). Simultaneous PET-MR phantom imaging was performed with different 89 Zr-FH concentrations. The MR quantitative image analysis determined the contrast-enhancing properties of FH. The signal-to-noise ratio (SNR) and full-width half-maximum (FWHM) of the line spread function (LSF) were calculated before and after coregistering the PET and MR image data. Results: High RCY (92%) and RCP (98%) of the [ 89 Zr]Zr-FH product was achieved. TEM analysis confirmed the 90 Zr atoms adsorption onto the SPION surface (≈ 10% average radial increase). Simultaneous PET-MR scans confirmed the capability of the [ 89 Zr]Zr-FH nanoplatform for this multi-modal imaging technique. Relative contrast image analysis showed that [ 89 Zr]Zr-FH can act as a dual-mode T1/T2 contrast agent. For co-registered PET-MR images, higher spatial resolution (FWHM enhancement ≈ 3) and SNR (enhancement ≈ 8) was achieved at a clinical dose of radio-isotope and Fe. Conclusion: Our results demonstrate FH is a highly suitable SPION-based platform for chelate-free labeling of PET tracers for hybrid PET-MR. The high RCY and RCP confirmed the robustness of the chelate-free HIR technique. An overall image quality gain was achieved compared to PET-or MR-alone imaging with a relatively low dosage of [ 89 Zr]Zr-FH. Additionally, FH is suitable as a dual-mode T1/T2 MR image contrast agent.

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Molecular Imaging with 68Ga Radio-Nanomaterials: Shedding Light on Nanoparticles

Cited by 18 publications

References 159 publications

Simultaneous in vivo PET/MRI using fluorine-18 labeled Fe3O4@Al(OH)3 nanoparticles: comparison of nanoparticle and nanoparticle-labeled stem cell distribution

Simultaneous in vivo PET/MRI using fluorine-18 labeled Fe3O4@Al(OH)3 nanoparticles: comparison of nanoparticle and nanoparticle-labeled stem cell distribution

Development and long-term evaluation of a new 68Ge/68Ga generator based on nano-SnO2 for PET imaging

<p>A Radio-Nano-Platform for T1/T2 Dual-Mode PET-MR Imaging</p>

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