Molecular Imaging of a New Multimodal Microbubble for Adhesion Molecule Targeting

Ahmed, Mona; Gustafsson, Björn; Aldi, Silvia; Dusart, Philip; Egri, Gabriella; Butler, Lynn M.; Bone, D.; Dähne, Lars; Hedin, Ulf; Caidahl, Kenneth

doi:10.1007/s12195-018-00562-z

Cited by 14 publications

(20 citation statements)

References 76 publications

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“…So far, effective encapsulation of different drugs, including anticancer 65 - 68 , RNA/DNA 69 - 71 , growth factors 72 , 73 , antigens 74 - 76 , and other bioactive substances 77 - 81 was demonstrated by means of polyelectrolyte capsules. Various multifunctional imaging agents were successfully fabricated using LbL technique as well 82 - 84 . Embedding of magnetite nanoparticles into the structure of polyelectrolyte capsules opens up the perspective for their application in MRI 44 , 85 - 88 as well as for improving drug delivery and controlled drug release 27 , 41 , 42 , 44 .…”

Section: Resultsmentioning

confidence: 99%

Biodegradable polyelectrolyte/magnetite capsules for MR imaging and magnetic targeting of tumors

Svenskaya¹,

Garello²,

Lengert³

et al. 2021

Nanotheranostics

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Rationale: The tireless research for effective drug delivery approaches is prompted by poor target tissue penetration and limited selectivity against diseased cells. To overcome these issues, various nano- and micro-carriers have been developed so far, but some of them are characterized by slow degradation time, thus hampering repeated drug administrations. The aim of this study was to pursue a selective delivery of magnetic biodegradable polyelectrolyte capsules in a mouse breast cancer model, using an external magnetic field. Methods: Four different kinds of magnetic polyelectrolyte capsules were fabricated via layer-by-layer assembly of biodegradable polymers on calcium carbonate templates. Magnetite nanoparticles were embedded either into the capsules' shell (sample S) or both into the shell and the inner volume of the capsules (samples C n S, where n is the number of nanoparticle loading cycles). Samples were first characterized in terms of their relaxometric and photosedimentometric properties. In vitro magnetic resonance imaging (MRI) experiments, carried out on RAW 264.7 cells, allowed the selection of two lead samples that proceeded for the in vivo testing on a mouse breast cancer model. In the set of in vivo experiments, an external magnet was applied for 1 hour following the intravenous injection of the capsules to improve their delivery to tumor, and MRI scans were acquired at different time points post administration. Results: All samples were considered non-cytotoxic as they provided more than 76% viability of RAW 264.7 cells upon 2 h incubation. Sample S appeared to be the most efficient in terms of T 2 -MRI contrast, but the less sensitive to external magnet navigation, since no difference in MRI signal with and without the magnet was observed. On the other side, sample C 6 S was efficiently delivered to the tumor tissue, with a three-fold T 2 -MRI contrast enhancement upon the external magnet application. The effective magnetic targeting of C 6 S capsules was also confirmed by the reduction in T 2 -MRI contrast in spleen if compared with the untreated with magnet mice values, and the presence of dense and clustered iron aggregates in tumor histology sections even 48 h after the magnetic targeting. Conclusion: The highlighted strategy of magnetic biodegradable polyelectrolyte capsules' design allows for the development of an efficient drug delivery system, which through an MRI-guided externally controlled navigation may lead to a significant improvement of the anticancer chemotherapy performance.

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Section: Resultsmentioning

confidence: 99%

Biodegradable polyelectrolyte/magnetite capsules for MR imaging and magnetic targeting of tumors

Svenskaya¹,

Garello²,

Lengert³

et al. 2021

Nanotheranostics

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“…d) Reproduced under the terms of the CC‐BY Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). [ 306 ] Copyright 2019, The Authors, published by Springer Nature.…”

Section: Core–shell Structures For Biomedical Applicationsmentioning

confidence: 99%

“…Poly(vinyl alcohol) (PVA) microbubbles with the diameter 3 µm have a LbL structure with a shell composed of layers of superparamagnetic iron oxide nanoparticles (SPIONs), metal chelating ligands, and a fluorophore‐labeled polyelectrolyte, and an outermost layer made of streptavidin (Figure 18d). [ 306 ] The streptavidin surface also enabled conjugation of biotinylated ligands (peptides or antibodies) for improving the specificity.…”

Section: Core–shell Structures For Biomedical Applicationsmentioning

confidence: 99%

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

et al. 2021

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Wireless nano‐/micromotors powered by chemical reactions and/or external fields generate motive forces, perform tasks, and significantly extend short‐range dynamic responses of passive biomedical microcarriers. However, before micromotors can be translated into clinical use, several major problems, including the biocompatibility of materials, the toxicity of chemical fuels, and deep tissue imaging methods, must be solved. Nanomaterials with enzyme‐like characteristics (e.g., catalase, oxidase, peroxidase, superoxide dismutase), that is, nanozymes, can significantly expand the scope of micromotors’ chemical fuels. A convergence of nanozymes, micromotors, and microfluidics can lead to a paradigm shift in the fabrication of multifunctional micromotors in reasonable quantities, encapsulation of desired subsystems, and engineering of FDA‐approved core–shell structures with tuneable biological, physical, chemical, and mechanical properties. Microfluidic methods are used to prepare stable bubbles/microbubbles and capsules integrating ultrasound, optoacoustic, fluorescent, and magnetic resonance imaging modalities. The aim here is to discuss an interdisciplinary approach of three independent emerging topics: micromotors, nanozymes, and microfluidics to creatively: 1) embrace new ideas, 2) think across boundaries, and 3) solve problems whose solutions are beyond the scope of a single discipline toward the development of micro‐bio‐chemo‐mechanical‐systems for diverse bioapplications.

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“…Multi-modal imaging specifically enables the study of biological processes, and has great potential for diseasespecific diagnostic evaluation in vivo. Caldahl [70] studied the possibility of targeting adhesion molecules on inflammation-activated endothelial cells and macrophages using polyvinyl alcohol-based MB developed for ultrasound, MRI and NI. These MBs permitted high ligand surface density and allowed the adhension of selective targeting of inflammatory markers such as ICAM-1, VCAM-1 and Eselectin, which offered a chance for diagnosis of inflammation ( Figure 9).…”

Section: Us-ni Combinationmentioning

confidence: 99%

“…MBs can be coupled to different antibodies via a streptavidin-biotin linkage (lower left panel). TEM (lower right panel) image of a MB with two layers of SPIONs as used in this study (Reproduced from Ref [70]. with the permission of Springer Ltd.) (color online).…”

mentioning

confidence: 99%

Functional micro/nanobubbles for ultrasound medicine and visualizable guidance

Zhang

et al. 2021

Sci. China Chem.

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Chemically functionalized gas-filled bubbles with a versatile micro/nano-sized scale have witnessed a long history of developments and emerging applications in disease diagnosis and treatments. In combination with ultrasound and image-guidance, micro/nanobubbles have been endowed with the capabilities of biomedical imaging, drug delivery, gene transfection and disease-oriented therapy. As an external stimulus, ultrasound (US)-mediated targeting treatments have been achieving unprecedented efficiency. Nowadays, US is playing a crucial role in visualizing biological/pathological changes in lives as a reliable imaging technique and a powerful therapeutic tool. This review retrospects the history of ultrasound, the chemistry of functionalized agents and summarizes recent advancements of functional micro/nanobubbles as US contrast agents in preclinical and trans-clinical research. Latest ultrasound-based treatment modalities in association with functional micro/nanobubbles have been highlighted as their great potentials for disease precision therapy. It is believed that these state-of-the-art micro/nanobubbles will become a booster for ultrasound medicine and visualizable guidance to serve future human healthcare in a more comprehensive and practical manner.

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Molecular Imaging of a New Multimodal Microbubble for Adhesion Molecule Targeting

Cited by 14 publications

References 76 publications

Biodegradable polyelectrolyte/magnetite capsules for MR imaging and magnetic targeting of tumors

Biodegradable polyelectrolyte/magnetite capsules for MR imaging and magnetic targeting of tumors

Micro‐Bio‐Chemo‐Mechanical‐Systems: Micromotors, Microfluidics, and Nanozymes for Biomedical Applications

Functional micro/nanobubbles for ultrasound medicine and visualizable guidance

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