Molecular identification, sequence analyses, tissue expression profile of a novel cattle gene-GBE1 in Yunnan cattle

Abstract

The complete coding sequences of a cattle gene -the glucan (1,4-alpha-), branching enzyme 1 (glycogen branching enzyme), GBE1, was amplifi ed using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the human, mouse and highly homologous cattle ESTs. Sequence analysis revealed that cattle GBE1 has high homology with the GBE1 from six species: dog (92%), cat (91%), horse (91%), human (89%), mouse (88%) and rat (88%). The phylogenetic tree analysis revealed that the cattle GBE1 has closer genetic relationships with the GBE1 of dog and cat. Tissue expression profi le analysis revealed that the cattle GBE1 gene was highly expressed in liver, moderately expressed in muscle and fat, weakly expressed in kidney, small intestine, large intestine, heart and lung. Our experiment established the primary foundation for further research on the cattle GBE1 gene.