Molecular identification of two Trichogramma species (Hymenoptera: Trichogrammatidae) in Korea

Jeong, Gilsang; Kim, Hwang‐Yong; Choi, Yang-Ho; Kim, Wontae; Park, Kwan-Ho; Bae, Sungwoo; Kim, Jonggill; Choi, Ji‐Young

doi:10.1016/j.aspen.2009.09.004

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…ITS2 is a highly conserved region within species, but varies substantially between species (Silva et al 1999;Stouthamer et al 1999b). Consequently, several molecular keys for the biological important Trichogramma species have been developed based on the length, nucleotide sequence and restriction fragment length polymorphism (PCR-RFLP) analysis of the ITS2 sequences (Chang et al 2001;Ciociola et al 2001;Dang et al 2005;Jeong et al 2010;Kumar et al 2009;Pinto et al 2002;Sappal et al 1995;Silva et al 1999;Stouthamer et al 1999b;Sumer et al 2009; Thomson et al 2003). In some cases, the ITS2 sequence cannot differentiate between species , which makes compatibility experiments based on the biological species concept (Mayr 1942) necessary (Gounou et al 2008;Pinto et al 1991Pinto et al , 1992Pinto andStouthamer 1994, Rosen 1986).…”

Section: Introductionmentioning

confidence: 99%

Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection

et al. 2011

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Egg parasitoids of the genus (Hymenoptera: Trichogrammatidae) are important natural enemies of many lepidopterous pests in agricultural and forest areas. Because the use of indigenous populations/species can significantly increase the biological control efficiency, the characterization of endemic populations and precise species identification is important. In this study, wasps were collected from parasitized eggs of several lepidopteran pests in Northern and Central Iran. Seven species were identified based on the morphology and the nucleotide sequence, size and restriction profile (PCR-RFLP) of the internal transcribed spacer 2 (ITS2) region of the rDNA of . Bezdenko, Marchal, Hartig, Westwood, Girault, Voegel, and Sorokina. This is the first report of in Iran. Additionally, natural populations were surveyed for the prevalence of . Two populations of were found to be infected by a single strain which was identified by using a multi-locus sequencing approach

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Section: Introductionmentioning

confidence: 99%

Iranian Trichogramma: ITS2 DNA characterization and natural Wolbachia infection

et al. 2011

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“…Trichogramma 속 ITS2 영역의 DNA 염기서열을 해독한 결과 종내 변이가 적 고 종간 염기서열 전체길이와 변이 차이가 커 종 구별을 위해 사 용될 수 있었다 Li et al, 2004). 그동안 많은 연구자들에 의해서 Trichogramma속 종들의 ITS2 전체 염기서열이 밝혀지고 GenBank 데이터베이스에 등록되고 있 으며 형태적 분류가 어려운 은폐종을 구별하여 왔다 (Amornsak et al, 1998;Silva et al, 1999;Stouthamer et al, 1999;Ciociola et al, 2001a, b;Li et al, 2004;Dang et al, 2005;Davies et al, 2006;Honda et al, 2006;Gariepy et al, 2007;Li, 2007;Kumar et al, 2009;Sumer et al, 2009;Jeong et al, 2010;Sumer et al, 2011;Karimi et al, 2012;Poorjavad et al, 2012;del Pino et al, 2013). 그러나 형태적으로 구별이 매우 힘든 T. minutum과 T.…”

Section: 방제하고자 하는 해충에 대해 효과가 좋은 기생벌 선발이 중요 한데 대량사육 과정에서 목적하지 않은 종이 섞unclassified

Molecular Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Egg Parasitoids of the Asian Corn Borer Ostrinia furnacalis, Based on ITS2 rDNA Sequence Analysis

Seo¹,

Jung²,

Park³

et al. 2014

Korean journal of applied entomology

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To identify the species of Trichogramma occurring in the corn fields of Korea as egg parasitoids of Ostrinia furnacalis, we sequenced the full-length of ITS2 nuclear rDNA from 112 parasitoids collected during this study. As a reference to distinguish species, we also retrieved full-length ITS2 sequences of 60 Trichogramma species from the NCBI GenBank database. On the basis of the size and 3′terminal sequence pattern of the ITS2 sequences, the Trichogramma samples collected in this study were divided into three groups (K-1, -2, and -3). Evolutionary distances (d) within and between groups based on ITS2 sequences were estimated to be ≤0.005 and ≥0.080, respectively. In the net average distance between groups or species, the d value between K-1 and T. ostriniae, K-2 and T. dendrolimi, and K-3 and T. confusum was the lowest, with values of 0.016, 0.001, and 0.002, respectively. In the phylogenetic tree, K-1 and K-2 were clustered with T. ostriniae and T. dendrolimi, respectively. However, K-3 was clustered with three different species, namely, T. confusum, T. chilonis, and T. bilingensis. NCBI BLAST results revealed that parasitoids belonging to K-1 and K-2 showed 99% identity with T. ostriniae and T. dendrolimi, respectively. Parasitoids in K-3 collected from Hongcheon showed 99-100% identity with T. confusum and T. chilonis, and one parasitoid in K-3 collected from Gochang had 98% identity with T. bilingensis, T. confusum, and T. chilonis. On the basis of these results, we infer that the species of Trichogramma collected in this study are closely related to T. ostriniae (K-1) and T. dendrolimi (K-2). However, it was not possible to distinguish species of K-3 using the ITS2 sequence alone.

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