Molecular Identification of Trissolcus japonicus, Parasitoid of the Brown Marmorated Stink Bug, by Species-Specific PCR

Chen, Maple N.; Santander, Ricardo D.; Talamas, Elijah J.; Jentsch, Peter; Bon, Marie‐Claude; Aćimović, Srđan G.

doi:10.3390/insects12050467

Cited by 5 publications

(2 citation statements)

References 44 publications

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“…In addition, insect sequences from internal transcribed spacers (ITS) or 5.8S-ITS2 rDNA are used for species-specific detection [27,28,45]. Species-specific molecular detection methods are rapid and cost-saving compared to analysis of morphological traits and reduce the risk of misidentification [31,46]. In the presented study, species-specific primers were designed on highly conserved parts of the COI gene of the target species as the COI gene was variable enough to distinguish P. leporinus from all other Auchenorrhyncha species reported from sugar beet fields.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Pentastiridius leporinus, the Main Vector of the Syndrome ‘Basses Richesses’ in Sugar Beet

Pfitzer

Varrelmann²,

Hesse³

et al. 2022

Insects

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Monitoring of Pentastiridius leporinus (Hemiptera: Auchenorrhyncha: Cixiidae), representing the main vector of the syndrome ‘basses richesses’ (SBR) disease in sugar beet is based on morphological identification. However, two other cixiid species, Reptalus quinquecostatus and Hyalesthes obsoletus with similar external characters are known to appear in sugar beet fields and are challenging to be distinguished from P. leporinus. We present a PCR-based method for species-specific detection of both male and female P. leporinus, directly after sweep net collection or after up to 18 months long term storage on sticky traps. Two methods of DNA template preparation, based on a commercial extraction kit or on simple grinding in phosphate-buffered saline (PBS) were compared. The latter method was also established for eggs and all five nymphal instars of P. leporinus from a rearing. Furthermore, in silico primer analysis showed that all Auchenorrhyncha species including far related species reported from sugar beet fields can be differentiated from P. leporinus. This was PCR-confirmed for the most common Auchenorrhyncha species from different German sugar beet fields. Sequence analysis of the P. leporinus mitochondrial cytochrome oxidase I gene (COI) amplicon showed a close relationship to COI from P. beieri but separated from the Reptalus and Hyalesthes species which are grouped into the same family Cixiidae. We present a sensitive, cost- and time-saving PCR-based method for reliable and specific detection of eggs and all nymphal instars, as well as male and female P. leporinus, after different methods of planthopper collection and template DNA template preparation that can be used in large scale monitoring assays.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Pentastiridius leporinus, the Main Vector of the Syndrome ‘Basses Richesses’ in Sugar Beet

Pfitzer

Varrelmann²,

Hesse³

et al. 2022

Insects

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“…With the advancement of molecular techniques, diverse molecular analyses are being utilized in biological control research. In particular, polymerase chain reaction (PCR)based detection methods are widely used to identify target organisms [23][24][25], confirm host ranges and trophic relationships [26][27][28][29], and detect gut content [30,31]. Since the traditional rearing and dissection of insects are laborious and lengthy experimental processes, the utilization of PCR-based methods allow investigators to rapidly detect and correctly identify target organisms [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Detection of Ochyromera ligustri (Coleoptera: Curculionidae: Curculioninae) in Ligustrum spp. (Oleaceae) Using Newly Developed PCR Primers

Kang,

Roda,

Misiaszek

et al. 2024

Insects

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Ligustrum spp. (Oleaceae) have become invasive species in the US and negatively affect native plant diversity and richness in forests. Ochyromera ligustri (Coleoptera: Curculionidae) is considered a potential biological control agent in the US because adults feed on the foliage and larvae are seed-feeders of Ligustrum spp. To discover the relationships between O. ligustri and Ligustrum spp., fruit dissections or rearing and field observations are required. In the current research project, novel PCR primers were developed to rapidly detect the DNA of O. ligustri in molecular analyses without rearing and observation. The developed PCR primers worked even with 0.01 ng of DNA and did not amplify the DNA of the other five curculionid species tested. When the novel primers were tested with three Ligustrum spp. species common in the southeastern US, the DNA of O. ligustri was detected from all three species. We expect that the novel primers will be utilized to find out the presence and impact of O. ligustri on Ligustrum spp rapidly and accurately.

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Comparison of Yellow and Blue Sticky Cards for Detection and Monitoring Parasitoid Wasps of the InvasiveHalyomorpha halys(Hemiptera: Pentatomidae)

Holthouse

Spears

Alston

2021

Journal of Insect Science

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The invasive Halyomorpha halys (Stål) is a significant agricultural and urban nuisance pest in many parts of the world. In North America, biological control of H. halys by parasitoid wasps in the families Scelionidae and Eupelmidae has shown promise. An effective technique for detection and monitoring native and exotic parasitoids is the deployment of yellow sticky cards; however, yellow cards also attract nontarget arthropods, reducing efficiency and accuracy of parasitoid screening. This study sought to identify an alternative yet effective trapping technique by comparing the number of target parasitoid wasps [Anastatus spp. Motschulsky (Hymenoptera: Eupelmidae), Telenomus spp. Haliday (Hymenoptera: Scelionidae), and Trissolcus spp. Ashmead (Hymenoptera: Scelionidae)] and arthropod bycatch on yellow and blue sticky cards deployed in urban, orchard, and vegetable landscapes in northern Utah from late May to early October in 2019 and 2020. Yellow sticky cards captured 54–72% more target parasitoids than blue cards from June through August in all three landscape types in both years; however, a positive correlation in parasitoid capture indicated blue cards detect target parasitoids, just in fewer numbers. Both card colors detected adventive Trissolcus japonicus (Ashmead) (Hymenoptera: Scelionidae) in initial findings of 2019, and in expanded locations of 2020. Furthermore, blue cards captured 31–48% less Diptera and nontarget Hymenoptera than yellow cards in both years across all three landscapes, translating to reduced card processing time and impacts to beneficial insect populations. Our results suggest that blue vs yellow sticky cards offer an alternative monitoring technique to survey for H. halys parasitoids.

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Molecular Identification of Trissolcus japonicus, Parasitoid of the Brown Marmorated Stink Bug, by Species-Specific PCR

Cited by 5 publications

References 44 publications

Molecular Detection of Pentastiridius leporinus, the Main Vector of the Syndrome ‘Basses Richesses’ in Sugar Beet

Molecular Detection of Pentastiridius leporinus, the Main Vector of the Syndrome ‘Basses Richesses’ in Sugar Beet

Detection of Ochyromera ligustri (Coleoptera: Curculionidae: Curculioninae) in Ligustrum spp. (Oleaceae) Using Newly Developed PCR Primers

Comparison of Yellow and Blue Sticky Cards for Detection and Monitoring Parasitoid Wasps of the InvasiveHalyomorpha halys(Hemiptera: Pentatomidae)

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