Molecular identification of Trichuris vulpis and Trichuris suis isolated from different hosts

Abstract

Trichuris suis was isolated from the cecum of two different hosts (Sus scrofa domestica -- swine and Sus scrofa scrofa -- wild boar) and Trichuris vulpis from dogs in Sevilla, Spain. Genomic DNA was isolated and internal transcribed spacers (ITS)1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The sequence of T. suis from both hosts was 1,396 bp in length while that of T. vulpis was 1,044 bp. ITS1 of both populations isolated of T. suis was 661 nucleotides in length, while the ITS2 was 534 nucleotides in length. Furthermore, the ITS1 of T. vulpis was 410 nucleotides in length, while the ITS2 was 433 nucleotides in length. One hundred fifty-four nucleotides were observed along the 5.8S gene of T. suis and T. vulpis. Intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all the individuals assayed. Sequence analysis of the ITSs and the 5.8S gene has demonstrated no sequence differences between T. suis isolated from both hosts (S. scrofa domestica -- swine and S. scrofa scrofa -- wild boar). Nevertheless, clear differences were detected between the ITS1 and ITS2 of T. suis and T. vulpis. Furthermore, a comparative molecular analysis between both species and the previously published ITS1-5.8S-ITS2 sequence data of Trichuris ovis, Trichuris leporis, Trichuris muris, Trichuris arvicolae, and Trichuris skrjabini was carried out. A common homology zone was detected in the ITS1 sequence of all species of trichurids.

Paleo-parasitological study on the soils collected from archaeological sites in old district of Seoul City

“…also pose differentiation difficulties. Distinguishing T. trichiura eggs from those of T. suis simply by morphological characteristics, for example, remains controversial, as some T. suis eggs morphologically resemble T. trichiura eggs (Schwartz, 1926;Martinez et al, 2003;Cutillas et al, 2007). Therefore, as was the case for Ascaris, we cannot rule out the possibility that some Trichuris eggs discovered in Yukjo Street might have originated from cattle roaming free there.…”

Morphological diversity of Trichuris spp. eggs observed during an anthelminthic drug trial in Yunnan, China, and relative performance of parasitologic diagnostic tools

“…Of note, the potential of T. vulpis to establish overt infections and reproduce in humans is currently unknown. Definitive identification of the observed eggs would have required molecular diagnostic tools, such as PCR (Cutillas et al, 2007). Isolation of adult worms from individuals excreting such eggs would have proved true human infections (Yoshikawa et al, 1989).…”

Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae)

“…(Beer and Lean, 1973;Cutillas et al, 2007;Khalafalla et al, 2011;Hotez et al, 2012). These parasites are transmitted via a direct, fecal-oral route.…”