Molecular Identification of Thripids Attacking Olive Groves at Ismailia, Egypt

Abstract

A little attention has been given on molecular identification of thrips (Insecta: Thysanoptera) in Egypt. Thus, the primers ITS4A, 28S rRNA, 18S rRNA and the primer pairs COI-1 + COI-2 + COI-3 were used to identify the three dominant thrips species (Haplothrips cahirensis karny; Dendrothrips eremicola Priesener and Hydatothrips kassimanus Priesener). The samples were collected from a private olive grove during 2013 and 2014 at Ismailia governorate, Egypt. DNA sequence data for 28S rRNA gene showed a positive response to H. cahirensis and D. eremicola only, while 18S rRNA reacted with all species. ITS4A and COI-1 + COI-2 + COI-3 showed a negative response to the studied species. DNA sequences of the species based on 18S rRNA were recorded in the Gene Bank. The phylogenetic tree of the three thrips species was given also.

Frontiers Approaches to the Diagnosis of Thrips (Thysanoptera): How Effective Are the Molecular and Electronic Detection Platforms?

“…Molecular markers are generally highly reliable for resolving species ambiguities that are often not possible with morphology-based taxonomy. In thrips, DNA markers such as COI, COII, COIII, ribosomal RNA (rRNA), internal transcribed spacers (ITS), random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), and simple sequence repeats (SSR) have been utilized successfully for species discrimination and phylogenetic analyses [ 13 , 14 , 44 , 45 , 46 ].…”

“…Ribosomal RNA-ITS markers have been used for detection of S. dorsalis , T. palmi , F. tritici (Fitch), F. intonsa (Trybom), F. cephalica (Crawford), H. cahirensis (Trybom), Dendrothrips eremicola Priesner, Kakothrips pisivorus (Westwood), Hydatothrips kassimianus (Priesener), and Ceratothripoides claratris (Shumsher) [ 46 , 114 , 115 , 116 , 117 , 118 ]. ITS has been used as a natural choice of nuclear marker to substantiate mitochondrial marker-based identification and allow multi-locus phylogenetic analyses of F. occidentalis , F. intonsa , F. fusca (Hinds), T. tabaci , and Megalurothrips distalis (Karny) [ 72 , 119 , 120 ].…”