Molecular Identification of Three &lt;i&gt;Aquilaria&lt;/i&gt; (Thymelaeaceae) Species through DNA Barcoding

Li, Qiwei; Yan, Haoran; Lin, Dan; Wang, Yesheng; He, Mengling; Zhang, Weimin; Gao, Xiaoxia; Zhu, Shuang

doi:10.1248/bpb.b18-00050

Cited by 13 publications

(6 citation statements)

References 15 publications

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“…When analyzing Aquilaria species, Lee et al concluded that the phylogenetic tree constructed with combination ITS2þtrnL-trnF was applicable for Aquilaria species . Li et al found that the phylogenetic tree constructed by ITSþmatK and ITSþtrnL-trnF is beneficial to the identification of three Aquilaria species (Li et al 2018). In this study, our results are differrent from those previous studies due to different fragments or combinations tend to have different identification effects for various Aquilaria species.…”

Section: Dna Barcoding Evaluation Of Aquilaria Species In Chinacontrasting

confidence: 85%

“…In addition, phylogenetic tree constructed by trnL-trnFþITS1 was found that could aggregate the DNA sequence of A. sinensis in GenBank (Jiao et al 2014). When comparing A. sinensis, A. yunnanensis and A. crassna, Li et al found that the combinations of ITSþmatK and ITSþtrnL-trnF are suitable for molecular identification of these three species (Li et al 2018). In the previous research, our group also found that matK fragments play an important role in Aquilaria species from multiple sources (Kang et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of Aquilaria species with distribution records in China using DNA barcode technology

Kang

2021

Mitochondrial DNA Part B

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Aquilaria species is one of the main plant resources that produce agarwood, which containing black resin with important economic and medicinal values. There are about 15 species known to the genus around the world, but only two can be found in China, i.e. A. sinensis and A. yunnanensis. In this study, A. sinensis and A. yunnanensis that endemic respectively to Hainan and Yunnan were sampled, on the basis of the investigation and observation of their main morphological features in plantation. Five primers, i.e. ITS2, matK, trnL-trnF1, trnL-trnF2, and trnH-psbA, were eventually selected for DNA barcoding. The results showed that the seed surface of A. sinensis is smooth or sparsely pubescent, and the seed appendages were long. While the seed surface of A. yunnanensis is densely covered with yellow hairs and the seed appendages are short. The trnL-trnF1 sequence fragment has significant intraspecific and interspecific genetic distances. However, the species identification success rate of ITS2þmatK combination was finally screened to be the highest, which was verified by the BBA method of TaxonDNA. The phylogenetic trees cluster analysis revealed that the classification of A. sinensis and A. yunnanensis is significant, and there is geographic isolation between the two species. Therefore, on the premise of accurate identification of plant morphological characters, ITS2þmatK combination can be used to accurately identify the Aquilaria species in China.

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Section: Dna Barcoding Evaluation Of Aquilaria Species In Chinacontrasting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Molecular identification of Aquilaria species with distribution records in China using DNA barcode technology

Kang

2021

Mitochondrial DNA Part B

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“…Since a 2-fragment combination is more efficient and reduces the cost of sequencing, the ITS2+ matK combination was selected to analyze the clustering of the phylogenetic trees. This differs from previous research on the DNA barcoding identification of Aquilaria species, as Lee et al concluded that the phylogenetic tree constructed by ITS2+ trnL-trnF was suitable for Aquilaria species [ 5 ], and Li et al found that the phylogenetic tree constructed by the combination of ITS+ matK and ITS+ trnL-trnF was conducive to species identification in three Aquilaria species [ 17 ]. Other research indicated that the trnL-trnF sequence could provide molecular identification of Aquilaria species [ 18 ].…”

Section: Discussioncontrasting

confidence: 64%

“…yunnanensis and A . crassna with three DNA barcodes and found two combinations (ITS+ matK and ITS+ trnL-trnF ) that could identify these three species [ 17 ]. Eurlings et al further suggested that the trnL-trnF fragment provides a new method of molecular identification for Aquilaria and Gyrinops species [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship and source species identification of 58 Qi-Nan germplasms of Aquilaria species in China that easily form agarwood

Kang

Liu

et al. 2022

PLoS ONE

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Recently, Qi-Nan germplasm, the germplasm of Aquilaria species that easily forms agarwood, has been widely cultivated in Guangdong and Hainan Provinces in China. Since the morphological characteristics of Qi-Nan germplasm are similar to those of Aquilaria species and germplasm is bred by grafting, it is difficult to determine the source species of this germplasm by traditional taxonomic characteristics. In this study, we performed a DNA barcoding analysis of 58 major Qi-Nan germplasms as well as Aquilaria sinensis, A. yunnanensis, A. crassna, A. malaccensis and A. hirta with 5 primers (nuclear gene internal transcribed spacer 2 (ITS2) and the chloroplast genes matK, trnH-psbA, rbcL and trnL-trnF). This field survey in the Qi-Nan germplasm plantations in Guangdong and Hainan Provinces aimed to accurately identify the source species of Qi-Nan germplasm. According to the results, ITS2 and matK showed the most variability and the highest divergence at all genetic distances. This ITS2+matK combination, screened for with TaxonDNA analysis, showed the highest success rate in species identification of the Qi-Nan germplasm. Clustering in the phylogenetic trees constructed with Bayesian inference and maximum likelihood indicated that the Qi-Nan germplasm was most closely related to A. sinensis and more distantly related to A. yunnanensis, A. crassna, A. malaccensis and A. hirta. Therefore, this study determined that the source species of the Qi-Nan germplasm is A. sinensis.

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“…Barcoding is useful for identifying the source species of natural medicines and has also been used to detect foreign matter contamination in natural medicines, especially those in small pieces or powder forms [3]. Whereas, there have been some reports of plant taxonomy using leaves of agarwood source species [4,5,6,7,8], few reports described identification of source species using wood pieces of 4 agarwood [9,10]. In this study, barcoding of DNA extracted from fresh and dried leaves as well as dried fruits of Aquilaria and Gyrinops plants was used to identify source species.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding for identification of agarwood source species using trnL-trnF and matK DNA sequences

Tanaka

Ito

2019

J Nat Med

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Agarwood is a type of resinous wood found in the trunks of Aquilaria, Gonystylus, and Gyrinops species [1]. High quality agarwood is extraordinarily expensive and therefore its source plant species have become depleted due to exploitation. In 2005, these species were added to Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora [1]. Because these wild agarwood resources have become depleted, commercial production of agarwood has long been a desirable goal. In addition, inauthentic agarwood is sometimes produced from non-agarwood species. Few reports have attempted to identify source species in order to distinguish genuine from false agarwood. In this study, DNA was extracted from putative agarwood samples collected from Japanese, Indonesian, Thai, and Vietnamese markets. The trnL-trnF region and matK gene were amplified from each sample by PCR to serve as DNA barcodes for identifying the plant species to which each sample belonged. One of the wood samples did not originate from a genuine agarwood species. Although some species were identified, sequence data for agarwood source species currently available in GenBank is insufficient to identify the species to which all of these putative agarwood samples belonged. Thus, positive identification of remaining samples will require further exploration.

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Molecular Identification of Three <i>Aquilaria</i> (Thymelaeaceae) Species through DNA Barcoding

Cited by 13 publications

References 15 publications

Molecular identification of Aquilaria species with distribution records in China using DNA barcode technology

Molecular identification of Aquilaria species with distribution records in China using DNA barcode technology

Genetic relationship and source species identification of 58 Qi-Nan germplasms of Aquilaria species in China that easily form agarwood

DNA barcoding for identification of agarwood source species using trnL-trnF and matK DNA sequences

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