Molecular identification of species of Physalis (Solanaceae) using a candidate DNA barcode: the chloroplast psbA–trnH intergenic region

Feng, Shi Ting; Jiao, Kaili; Zhu, Yongzhong; Wang, Hongfen; Jiang, Mengying; Wang, Huizhong

doi:10.1139/gen-2017-0115

Cited by 21 publications

(16 citation statements)

References 30 publications

(46 reference statements)

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“…Specimens of Physalis are difficult to identify to species morphologically. A recent study shows that the chloroplast barcode psbA-trnH was able to identify species of this genus (Feng et al, 2018). In our study, the DNA barcode that showed higher variability within Physalis was psbA-trnH, and when concatenated barcodes were used better discrimination was obtained ( Fig.…”

Section: Discussionsupporting

confidence: 57%

DNA Barcoding of the Solanaceae Family in Puerto Rico Including Endangered and Endemic Species

Rosario¹,

Padilla²,

Martínez³

et al. 2019

J. Amer. Soc. Hort. Sci.

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The Solanaceae family is one of the largest and well-distributed plant families in the world. It contains species of agricultural and economical importance, such as Solanum tuberosum, Solanum melongena, Solanum lycopersicum, Nicotiana tabacum, and Capsicum annuum. In Puerto Rico, there are ≈46 species of Solanaceae of which six are endemic: Brunfelsia densifolia, Brunfelsia lactea, Brunfelsia portoricensis, Goetzea elegans, Solanum ensifolium, and Solanum woodburyi. Our objective was to use DNA barcoding to identify the Solanaceae species in Puerto Rico, including the endemics, and to assess the species relationships between them. To accomplish our objective, two chloroplast regions (psbA-trnH and matK) and a nuclear region [internal transcribed spacer (ITS)] were assessed. Pairwise distance and phylogenetic analysis demonstrate that DNA barcoding can be used to discriminate at the species level among these taxa in Puerto Rico. For all three markers, the genus that showed the highest pairwise distance between represented species was Solanum, whereas the genus that displayed the least was Capsicum. Phylogenetic trees of single and concatenated regions were generated from sequences obtained in this study and from data downloaded from the National Center for Biotechnology Information database. Our results show that this technique can be used to identify species with one, two, or three combinations of DNA barcode markers depending on the taxon. In addition, this is the first study to include the endemic species S. woodburyi in a molecular phylogenetic analysis, and it was found to have a close relationship with S. ensifolium, also endemic to Puerto Rico, and to Solanum bahamense from the Bahamas and Greater Antilles. Therefore, we suggest that S. woodburyi might be part of the Bahamense clade.

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Section: Discussionsupporting

confidence: 57%

DNA Barcoding of the Solanaceae Family in Puerto Rico Including Endangered and Endemic Species

Rosario¹,

Padilla²,

Martínez³

et al. 2019

J. Amer. Soc. Hort. Sci.

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“…The genus Physalis of family Solanaceae is among the largest genera in subfamily Solanoideae, with about 100 species, although the estimation of the species within the genus varies considerablyfrom 75 to 120 (Martínez 1998;Feng et al 2018). The genus is generally recognized by the inflated balloon or lantern-like calyx (husk, fruit basket), which completely envelopes the berry, protecting it against insects, birds, diseases and adverse climatic conditions (Puente et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Bitter); by fruit and seed morphology (berries have either juicy or rather dry pericarp, the colour of ripe berries varying from green to yellow to orange or purple; seed colour varying from light yellow to brown, and seed sizefrom 0.6 to 3.0 mm) (Kindscher et al 2012;Martínez 1998;Puente et al 2011;Sharma et al 2015). On the other hand, several Physalis species have highly similar morphological traits and misidentification in the genus is common, as in the case of P. minima L., which is confused with P. angulata L. or P. pubescens L. in traditional Chinese medicine preparations, or P. angulata being confused with P. peruviana L. (Feng et al 2018). Moreover, many of the common names used in different regions of the world may be applied to more than one Physalis species, for example "goldenberry", "gooseberry", "tomatillo", "Chinese or Japanese lantern" or the French "amour en cage" (love-in-a-cage).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical composition and biological activity of Physalis spp.: A mini-review

Mazova

Popova

Stoyanova

2020

FSAB

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The main objective of this mini-review was to synthesize recent data about the phytochemical composition, the nutritional properties, and the biological and pharmacological activities of a now cosmopolitan genus, Physalis (Solanaceae), being in the focus of intensive research over the last two decades. Six Physalis species with nutritional and pharmacological promise are considered in particular – P. peruviana L., P. philadelphica Lam., P. ixocarpa Brot. ex Horm., P. angulata L., P. pubescens L., and P. alkekengi L. Summarized contemporary data on the metabolite profile and the biological activities of Physalis species support their century-long use in traditional medicine and human nutrition. The fruit represent a rich source of minerals, vitamins, fibers, carotenoids, proteins, fructose, sucrose esters, pectins, flavonoids, polyphenols, polyunsaturated fatty acids, phytosterols and many other beneficial nutrients. Individual phytochemicals and complex fractions isolated from Physalis plants demonstrate various biological and pharmacological activities, the most promising of which include antimicrobial, antioxidant, anti-diabetic, hepato-renoprotective, anti-cancer, anti-inflammatory, immunomodulatory and others. Most of these activities are associated with the presence of flavonoids, phenylpropanoids, alkaloids, physalins, withanolides, and other bioactive compounds. The accumulated data disclose the potential of Physalis spp. as highly functional foods, as profitable crops for many regions over the world, and as sources of valuable secondary metabolites for phytopharmacy, novel medicine and cosmetics. Information provided by this review is also important for a more intensive promotion of Physalis species in Bulgaria and for future studies on their composition and benefits.

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“…Several DNA barcode regions, such as internal transcriber spacers ( ITS1 and ITS2 ) and plastidial genes psbA‐trnH , trnL‐F , matK , and rbcL , have become the most promising loci in plant differentiation . The psbA‐trnH sequence has a faster evolution rate and higher species‐identification efficiency, and it is becoming a commonly used and reliable chloroplast barcode gene in the study of plant molecular phylogeny . The use of DNA barcodes with psbA‐trnH primers and high‐resolution melting (HRM) analysis is a cost‐effective approach for the identification of Uvaria sp.…”

Section: Introductionmentioning

confidence: 99%

“…21 The psbA-trnH sequence has a faster evolution rate and higher species-identification efficiency, and it is becoming a commonly used and reliable chloroplast barcode gene in the study of plant molecular phylogeny. 22 The use of DNA barcodes with psbA-trnH primers and high-resolution melting (HRM) analysis is a costeffective approach for the identification of Uvaria sp. and Chinese medicine species.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

et al. 2020

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Background Walnut (Juglans regia L.) is one of the most widely cultivated nuts. Walnut milk beverage is very popular in China due to its nutritional value. However, adulterated walnut milk ingredients have been detected in the Chinese market. Peanut and soybean are sold at much lower prices than walnut and are reported to be commonly used for adulteration in the industrial chain of walnut milk production. The purpose of this study is therefore to develop an accurate and efficient method for detecting the authenticity of the raw materials used in walnut milk beverage. Results DNA barcoding and high‐resolution melting (HRM) analyses were used to identify common adulterated raw ingredients such as peanut and soybean in commercial walnut milk beverage samples. The chloroplast psbA‐trnH gene was used for sequencing, and HRM analysis was performed. We also prepared experimental mixtures, in the laboratory, with different quantities of walnut, peanut, and soybean. High‐resolution melting analysis of the experimental mixtures clearly distinguished all of them. The results revealed that most of the walnut milk beverage samples fell in the same cluster of walnut species. Several samples fell in the peanut cluster, confirming that they were adulterated products. Conclusion The results revealed that HRM analysis based on the psbA‐trnH barcode sequence can be used to identify raw ingredients in walnut milk beverages. © 2020 Society of Chemical Industry

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Molecular identification of species of Physalis (Solanaceae) using a candidate DNA barcode: the chloroplast psbA–trnH intergenic region

Cited by 21 publications

References 30 publications

DNA Barcoding of the Solanaceae Family in Puerto Rico Including Endangered and Endemic Species

DNA Barcoding of the Solanaceae Family in Puerto Rico Including Endangered and Endemic Species

Phytochemical composition and biological activity of Physalis spp.: A mini-review

DNA barcoding coupled with high‐resolution melting analysis for nut species and walnut milk beverage authentication

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