Molecular identification of species and ploidy of Carassius fishes in Lake Biwa, using mtDNA and microsatellite multiplex PCRs

Mishina, Tomobumi; Takada, Mikumi; Takeshima, Hirohiko; Nakano, Mitsunori; Tabata, Ryoichi; Nishida, Mutsumi; Watanabe, Katsutoshi

doi:10.1007/s10228-014-0388-9

Cited by 10 publications

(17 citation statements)

References 22 publications

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“…); and the cyprinid Carassius buergeri grandoculis (see Mishina et al. )), known mtDNA introgression (the gobiid Rhinogobius sp. BW ( sensu Akihito et al.…”

Section: Methodsmentioning

confidence: 99%

“…For locality codes, see Tables S1 and S2. polyploidy (e.g., the cobitids Cobitis magnostriata and Cobitis minamorii oumiensis (see Saitoh 2000;Kitagawa et al 2005); and the cyprinid Carassius buergeri grandoculis (see Mishina et al 2014)), known mtDNA introgression (the gobiid Rhinogobius sp. BW (sensu Akihito et al 2013;Yamasaki et al 2015)), or the closest relative was uncertain (the cyprinid Ischikauia steenackeri; Takeuchi and Hosoya 2011).…”

Section: Classificationmentioning

confidence: 99%

“…The haplotype frequencies for populations are available from GEDIMAP (http://gedimap.zool.kyoto-u.ac.jp/; Watanabe et al 2010a) under accession numbers P2000-2195. For population genetic analyses, the sequence data of some species were drawn from Harada et al (2002), Tominaga et al (2009Tominaga et al ( , 2015, Watanabe et al (2010b), Komiya et al (2011), Kakioka et al (2013), Tabata and Watanabe (2013), and Mishina et al (2014).…”

Section: Mtdna Sequencingmentioning

confidence: 99%

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Phylogeny and historical demography of endemic fishes in Lake Biwa: the ancient lake as a promoter of evolution and diversification of freshwater fishes in western Japan

Tabata

Kakioka

Tominaga

et al. 2016

Ecology and Evolution

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To elucidate the origins of the endemic fish of Lake Biwa, an ancient lake in Japan, and the role of the lake in the diversification of freshwater fish in western Japan, we established a molecular phylogenetic framework with an absolute time scale and inferred the historical demography of a large set of fish species in and around the lake. We used mtDNA sequences obtained from a total of 190 specimens, including 11 endemic species of Lake Biwa and their related species, for phylogenetic analyses with divergence time estimations and from a total of 2319 specimens of 42 species (including 14 endemics) occurring in the lake for population genetic analyses. Phylogenetic analysis suggested that some of the endemic species diverged from their closest relatives earlier (1.3–13.0 Ma) than the period in which the present environmental characteristics of the lake started to develop (ca. 0.4 Ma), whereas others diverged more recently (after 0.4 Ma). In contrast, historical demographic parameters suggested that almost all species, including endemic and nonendemic ones, expanded their populations after the development of the present lake environment. In phylogeographic analyses, common or very close haplotypes of some species were obtained from Lake Biwa and other regions of western Japan. The phylogenetic and historical demographic evidence suggests that there was a time lag between phylogenetic divergence and population establishment and that phenotypic adaptation of some endemic species to the limnetic environment occurred much later than the divergences of those endemic lineages. Population structure and phylogeographic patterns suggest that Lake Biwa has functioned not only as the center of adaptive evolution but also as a reservoir for fish diversity in western Japan.

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“…); and the cyprinid Carassius buergeri grandoculis (see Mishina et al. )), known mtDNA introgression (the gobiid Rhinogobius sp. BW ( sensu Akihito et al.…”

Section: Methodsmentioning

confidence: 99%

Section: Classificationmentioning

confidence: 99%

Section: Mtdna Sequencingmentioning

confidence: 99%

See 1 more Smart Citation

Phylogeny and historical demography of endemic fishes in Lake Biwa: the ancient lake as a promoter of evolution and diversification of freshwater fishes in western Japan

Tabata

Kakioka

Tominaga

et al. 2016

Ecology and Evolution

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“…Japanese crucian carps into two species, C. cuvieri and C. auratus were classified [2], and others have confirmed this classification using molecular markers [19,20,21]. Similarly, C. auratus, the species endemic to Korea, and C. cuvieri, the exotic species, are two distinct species that live in Korean freshwaters.…”

Section: Introductionmentioning

confidence: 97%

“…In order to overcome the limitations in the morphological identification of Carassius species, molecular genetic markers, such as random amplified polymorphic DNA [17], microsatellite DNA [18,19], and partial sequences of mitochondrial DNA [20][21][22] have been used for the identification of species, ploidy, clonal lineages, and phylogenetic relationships. Because of its small size, maternal inheritance, and rapid rate of evolution, mtDNA is often used as a genetic marker in phylogenetic and evolutionary studies in vertebrates, including fish [23].…”

Section: Introductionmentioning

confidence: 99%

Genetic Differentiation of Carassius auratus and C. cuvieri by the Cytochrome C Oxidase I Gene Analysis

Noh¹

2014

J Aquac Res Development

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It is estimated that 27 bony fish species have been introduced into Korean freshwaters for aquaculture and the enhancement of fishery resources. Among them, Carassius cuvieri has completely adapted and is widely distributed throughout Korean freshwater ecosystems. In this study we investigated the genetic relationship between C. cuvieri and the closely related native crucian carp species Carassius auratus. Nucleotide sequence variation within the mtDNA cytochrome c oxidase I (COI) gene was used to study the genetic divergence and phylogenetic relationships among the species and to identify their taxonomic status in the family Cyprinidae. We compared partial COI gene sequences (630 base pairs) from three populations of C. auratus and three populations of C. cuvieri. A total of 46 variable sites were identified from 163 individuals, yielding 23 haplotypes. The two species showed very close genetic relationship with 95% sequence identity in the COI gene, the frequency and distribution of haplotypes showed clear separation, suggesting divergent evolution of these two species. Therefore, despite the morphological similarity and phylogenetic closeness, the populations of these two species should be regarded as separate management units in the ongoing recovery program.

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The first record of spawning-season homing of gin-buna crucian carp Carassius sp. to a lakeside biotope connected to Lake Biwa confirmed via acoustic telemetry and egg surveys

2022

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Molecular identification of species and ploidy of Carassius fishes in Lake Biwa, using mtDNA and microsatellite multiplex PCRs

Cited by 10 publications

References 22 publications

Phylogeny and historical demography of endemic fishes in Lake Biwa: the ancient lake as a promoter of evolution and diversification of freshwater fishes in western Japan

Phylogeny and historical demography of endemic fishes in Lake Biwa: the ancient lake as a promoter of evolution and diversification of freshwater fishes in western Japan

Genetic Differentiation of Carassius auratus and C. cuvieri by the Cytochrome C Oxidase I Gene Analysis

The first record of spawning-season homing of gin-buna crucian carp Carassius sp. to a lakeside biotope connected to Lake Biwa confirmed via acoustic telemetry and egg surveys

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