Molecular Identification of Some Respiratory Pathogens from Broiler Chickens in Sharkia Governorate, Egypt

Megahed, M.; Ghanem, Ibrahim A.; Hussein, Hussein Awad; Abdelaziz, Walaa S.

doi:10.21608/zvjz.2020.20584.1091

Cited by 2 publications

(4 citation statements)

References 21 publications

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“…Here, this study focused on bird flocks, especially broiler chickens, which demonstrated varying degrees of respiratory distress, nervous signs, greenish diarrhea, whitish diarrhea, and variable mortalities, as recorded by Megahed et al [29]. Accordingly, the postmortem examination of the investigated flocks revealed various extents of airsacculitis, pericarditis, and perihepatitis, as mentioned by Bharathi et al and Emam et al [30,31].…”

Section: Discussionmentioning

confidence: 82%

“…In the present study, to elucidate the best straightforward scheme for the MG strain analysis, different conventional and molecular approaches for MG infection detection in commercial poultry farms were evaluated. Here, this study focused on bird flocks, especially broiler chickens, which demonstrated varying degrees of respiratory distress, nervous signs, greenish diarrhea, whitish diarrhea, and variable mortalities, as recorded by Megahed et al [ 29 ]. Accordingly, the postmortem examination of the investigated flocks revealed various extents of airsacculitis, pericarditis, and perihepatitis, as mentioned by Bharathi et al and Emam et al [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thirty-five PCR products representing eleven infected poultry flocks of different species targeting six gene loci ( mgc2 , ugpA , mraW , lgT , DUF31196, and atpG ) were partially sequenced. In addition to molecular identification, the gene target sequence analysis of the mgc2 was also widely used [ 12 , 29 , 46 ]. The tree comparison revealed the limitation of the phylogeny of the mraW , IgT, and DUF31196 in the classification of the MG-studied isolates into different clusters, probably due to their conservative character [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

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Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains

Al-baqir,

Hassanin,

Al-Rasheed

et al. 2023

Pathogens

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Infections with Mycoplasma gallisepticum (MG) in poultry are associated with a wide range of disease conditions, including those affecting the respiratory and reproductive systems. The purpose of this study was to endorse the more sensitive diagnostic scheme for MG infection and identify the best molecular marker for MG phylogenetic analysis using six housekeeping genes: mgc2, mraW, atpG, ugpA, DUF31196, and lgT. For these purposes, 55 poultry flocks of different species were screened using either qRT-PCR or PCR techniques analogous to conventional culturing from non-cultured and cultured swabs on PPLO broth. The rate of MG positivity was the highest when using qRT-PCR from cultured broth (89.0%) and the lowest when using conventional culturing (34.5%). Compared to qRT-PCR from broth, statistical analysis using the Roc curve in MedCalc statistical software showed that the PCR schemes (qRT-PCR from swabs and PCR from swabs and broth) performed better than conventional culturing in terms of sensitivity, accuracy, and area under the curve (AUC), suggesting that they may be more reliable schemes. Further support was added by Cohen’s kappa test, showing moderate agreement between the molecular approaches. Among the six screened genes, mgc2 and mraW had the highest detection rates (69% and 65.4%, respectively). The comparative phylogenetic analysis revealed that mgc2 or atpG gene sequences distinguished MG isolates into different clades with high discriminatory power.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains

Al-baqir,

Hassanin,

Al-Rasheed

et al. 2023

Pathogens

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“…So, circulated M.gallisepticum strain vertically transmitted from breeder the main reservoir to day old chicks and still silent not make any disease in chickens until immuno suppression occurs due to viral vaccination, bacterial, viral infection or environmental stressors as bad hygiene or defect in biosafety measures .Twelve MG isolates and four MS isolates were characterized by culture, biochemical tests and PCR. MG isolates were50% by PCR where MS isolates were16.7% in Egypt 2020 [25]. For the purpose of this investigation, three identified M. gallisepticum strains, Zagazig/2014/1 (Mk310102), Zagazig/2014/2 (MK 310103), and Zagazig/2017 (MK310101), were sequenced and phylogenetically analysed.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Avian Mycoplasma with Special Reference to Antibiotic Sensitivity in Egypt

Hider,

E. Abdelaziz,

M. Helmy

et al. 2025

Egyptian Journal of Veterinary Sciences

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VIAN MYCOPLASMOSIS is one of the most significant financial threats affecting the global poultry business so, convenient approach for detecting etiological agents; Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS)to characterize the circulating field strains and determine their antimicrobial susceptibility profiles. A study conducted between 2017 to 2022 in Kafrelshekh, Sharkia and Dakahlia, governorates in Egypt on different avian sectors, Twelve MG and four MS isolates were identified by culture and confirmed by PCR. Partial sequence of mgc2 gene of two MG isolates from turkey (MGB.T.) and (MGW.T.) was submitted on gene bank under accession no.OM632677.and OM632678 respectively and revealed that there are two different MG circulating strains in turkey, for determining the antimicrobial susceptibility profiles , ten MG, one MS isolates from different avian sector were tested against eight antimicrobials for determination of lowest MIC, three antibacterials, tiamulin (0.009μg/ml), tilmicosin (0.039μg/ml) and tylosin (0.019μg/ml), offered the lowest MICs of all efficient medications .MS isolate was sensitive for the eight antimicrobials, most MG isolates were sensitive to ciprofloxacin and streptomycin while Lincospectin has intermediate effect. Isolate MG11from baladi chicks was completely resistant to all antimicrobial. The two MG isolates from turkey were resistant to (erythromycin and tylosin), so, further investigation for macrolides resistant genes detection is required. The two MG isolates from turkey were resistant to (erythromycin and tylosin), so, further investigation for macrolides resistant genes detection is required. In conclusion, the present study confirms the presence of MG infection in turkey and chicken flocks of Sharkia and Dakahlia governorates.

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Molecular Identification of Some Respiratory Pathogens from Broiler Chickens in Sharkia Governorate, Egypt

Cited by 2 publications

References 21 publications

Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains

Mycoplasmosis in Poultry: An Evaluation of Diagnostic Schemes and Molecular Analysis of Egyptian Mycoplasma gallisepticum Strains

Molecular Characterization of Avian Mycoplasma with Special Reference to Antibiotic Sensitivity in Egypt

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