Molecular Identification of Shiga-Toxin Producing and Enteropathogenic &amp;lt;i&amp;gt;Escherichia coli&amp;lt;/i&amp;gt; (STEC and EPEC) in Diarrheic and Healthy Young Alpacas

Mori, Lorena; Perales, C Rosa; Rodríguez, Jorge; Shiva, Carlos; Koga, Ysabel; Choquehuanca, Geraldine; Palacios, Cesar

doi:10.4236/aim.2014.47043

Cited by 6 publications

(2 citation statements)

References 17 publications

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“…In Peru, alpacas are the principal economic resource of the Andean communities and they suffer from diarrhea associated with pathogenic Escherichia coli strains, especially enterohemorrhagic E. coli (EHEC) and enteropathogenic E. coli (EPEC) strains ( 1 , 2 ). The gastrointestinal tract of animals, mainly cattle, is considered the most important reservoir of EHEC ( 3 ); this pathogen is responsible for serious human diseases, such as hemolytic-uremic syndrome (HUS) ( 3 ), which expresses Shiga toxins.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains Isolated from Alpacas in Peru

et al. 2018

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Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains Isolated from Alpacas in Peru

et al. 2018

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“…En alpacas y llamas se han desarrollado diversos estudios para la identificación de factores de virulencia asociados a los patotipos de E. coli presentes. Así, se han descrito los patotipos EPEC, ETEC y EHEC como los más frecuentes a partir de aislados de crías y adultos, tanto en casos de animales con diarreas (enfermos) y sin diarrea (Cid et al, 2012;Luna et al, 2012;Mori et al, 2014;Rosadio et al, 2012). A la vez, se han descrito algunos serotipos fimbriales y su asociación con rotavirus en crías de alpacas con diarreas y en alpacas clínicamente sanas (Morales y Paredes, 2007).…”

Section: Introductionunclassified

Desarrollo de una proteína recombinante fimbrial F17 de Escherichia coli y respuesta inmune frente a células mononucleares periféricas sanguíneas (PBMC) de alpaca

Siuce

Maximiliano

Hurtado

et al. 2021

Rev. investig. vet. Perú

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Escherichia coli está asociada a diarreas que pueden producir la muerte en crías de alpacas de temprana edad. Patotipos de E. coli utilizan las fimbrias para adherirse al hospedero en la primera etapa de la patogénesis. Por ello, el presente estudio tuvo como objetivo desarrollar y producir una proteína recombinante de la adhesina fimbrial F17 de E. coli y evaluar su inmunogenicidad en células mononucleares periféricas de sangre (PBMC) de alpaca. Se utilizó a BL21 como vector de expresión, se purificó la proteína recombinante mediante cromatografía de afinidad inmovilizada y se evaluó la producción de citoquinas de PBMC a las 48 y 72 horas posterior al desafío con F17. Los resultados indican un aumento significativo de la producción de citoquinas de Th1/Th2, predominantemente IFN-γ e IL-4. Por lo tanto, la mezcla BL21-F17 podría ser considerada como un potencial candidato vacunal para la prevención de las diarreas en alpacas producidas por E. coli.

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Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing

Mostafavi

Peerayeh

Mobarez

et al. 2019

Journal Cellular Physiology

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Pathogenic and drug‐resistant strains of Escherichia coli (E. coli) O25b‐B2‐ST131, O15:H1‐D‐ST393, and CGA (clonal group A) clonal groups have spread worldwide. This study aimed at determining E. coli epidemic clonal groups, their virulence factors, biofilm formation, neutrophils apoptosis, and antimicrobial resistance pattern of uropathogenic E. coli. A total of 95 CTX‐M‐1‐producing E. coli clinical isolates were enrolled. E. coli O25b‐B2‐ST131, CGA, and O15:K52:H1 were identified by serotyping and phylogrouping and allele‐specific polymerase chain reaction‐based assay. Antibiotic susceptibility, biofilm formation, hemolysis, and human serum bactericidal assay were performed. Neutrophil apoptosis was assayed by flow cytometry. Nine E. coli clonal groups including six O25b‐B2‐ST131 strains, two CGA, and one O15:K52:H1‐D‐ST393 strains were detected. One O25b‐B2‐ST131 isolate was a strong biofilm‐producer. Three ST131 isolates had type I fimbriae. Furthermore, all the CGA and O15:K52:H1 and three of ST131 isolates harbored the P fimbriae. The virulence genes ompT, fimH, and traT were detected among all the clonal groups. The apoptosis was induced by O25b‐B2‐ST131, CGA, and O15:K52:H1 E. coli. There was no significant difference regarding apoptosis induction among clonal groups. Furthermore, the presence of the cdt, usp, and vat genes was significantly associated with the apoptosis of neutrophils by O25b‐B2‐ST131, CGA, and O15:K52:H1‐D‐ST393 clonal groups.

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Molecular Identification of Shiga-Toxin Producing and Enteropathogenic <i>Escherichia coli</i> (STEC and EPEC) in Diarrheic and Healthy Young Alpacas

Cited by 6 publications

References 17 publications

Draft Genome Sequences of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains Isolated from Alpacas in Peru

Draft Genome Sequences of Enterohemorrhagic and Enteropathogenic Escherichia coli Strains Isolated from Alpacas in Peru

Desarrollo de una proteína recombinante fimbrial F17 de Escherichia coli y respuesta inmune frente a células mononucleares periféricas sanguíneas (PBMC) de alpaca

Characterization of uropathogenic E. coli O25b‐B2‐ST131, O15:K52:H1, and CGA: Neutrophils apoptosis, serum bactericidal assay, biofilm formation, and virulence typing

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