Molecular Identification of Sarcoptes Scabiei Var. Cuniculi From Surabaya and Malang Regions of East Java

Abstract

Scabies is a zoonotic skin disease caused by Sarcoptes scabiei mites. As an emerging/re-emerging parasitic disease, scabies represents a significant global threat to both human and animal health. Numerous cases of scabies in

Clinical and molecular detection of Sarcoptes scabiei in the Iraqi camels

“…Interestingly, it is mentioned that the mange of camels can predominately be identified based on clinical signs (21). Therefore, these observations are consistent with the clinical signs of mange caused by S. scabies, where they agreed with other previous studies (21)(22)(23)(24). The molecular diagnosis (cPCR) based on COX1 shows that 29.41% of the examined camels were infested with S. scabiei.…”

“…The molecular diagnosis (cPCR) based on COX1 shows that 29.41% of the examined camels were infested with S. scabiei. This gene is previously used in some studies to identify the S. scabiei parasite in different animals (23)(24)(25). The current finding agrees with a study done in Najaf abattoir and nomadic areas for detecting sarcoptic mange in camels, where the percentage was 25.9% (25).…”

Sequence analysis of the cytochrome c oxidase subunit 1 gene of Sarcoptes scabiei isolated from goats and rabbits in East Java, Indonesia

“…The tube was removed and changed; then 50 µl of elution buffer (AE buffer) was added to the final DNA pellet. After incubation at 15-25°C for 1 min, the contents were centrifuged at 8,000 G for 1 min to collect the DNA [15,17].…”

“…The amplified PCR product was electrophoresed on a 2% agarose gel in 1× Tris-borate-EDTA buffer with pH 8.3 and 0.03 μl ethidium bromide. The gel was visualized under ultraviolet light [15,19].…”

“…The COX-1 fragment is useful for reconstructing phylogenetic diversity in evolutionary branches below the species level. Mitochondria undergo rapid evolution, but there are parts of it, such as the COX-1 fragment that experience low evolution, rates and can, thus, be used as genetic characters [9,15,16].…”