Molecular Identification of Sarcocystis spp. in Sheep and Cattle by PCR-RFLP from Southwest of Iran

Rahdar, Mahmoud; Kardooni, Tahereh

doi:10.5812/jjm.12798

Cited by 13 publications

(13 citation statements)

References 23 publications

(22 reference statements)

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“…Based on the data reported by Nourani et al [34], the thin-walled sarcocysts in cattle were consistent with S. cruzi with higher prevalence, and the thickwalled sarcocysts could represent S. hominis with lower prevalence. Both S. cruzi and S. tenella were found at higher prevalence rates of 100% and 80% by Daryani et al [20] and Rahdar and Kardooni [42], respectively.…”

Section: Discussionmentioning

confidence: 94%

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Abdullah

2021

Vet World

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Background and Aim: Sarcocystosis is a zoonotic infection caused by various species of Sarcocystis organisms with a worldwide geographic distribution. This study investigated the presence of Sarcocystis organisms in cattle and sheep slaughtered at an abattoir in Sulaimani Province in North Iraq. Materials and Methods: A total of 130 muscle samples were collected during May, June, and July of 2020, including 80 samples from sheep and 50 samples from cattle. Samples were examined visually for macrosarcocysts. The peptic digestion method was used to analyze fresh muscle tissue samples for detecting microsarcocysts followed by microscopic examination. Furthermore, muscle samples were fixed and stained with hematoxylin and eosin for histopathological examination. Results: In the gross examination, macroscopic cysts were not detected in both cattle and sheep; hence, all the prevalence data were obtained through microscopic observation of muscle samples. The peptic digestion method revealed the presence of banana-shaped bradyzoites in 90% and 92.5% of slaughtered cattle and sheep muscle samples, respectively. Organ-wise prevalence revealed that 95% and 92% of esophageal samples of sheep and cattle contained Sarcocystis spp., respectively. Moreover, 90% and 88% of sheep and cattle diaphragms were respectively infected. Histopathological examination of tissue sections revealed two morphologically distinct types of microsarcocysts, including thin-walled and thick-walled, in both sheep and cattle. Conclusion: The suspected Sarcocystis spp. were Sarcocystis tenella and Sarcocystis arieticanis in sheep and Sarcocystis cruzi and Sarcocystis bovifelis or Sarcocystis hominis in cattle. Infective stages of different Sarcocystis spp. are widespread in the study area environment.

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Section: Discussionmentioning

confidence: 94%

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Abdullah

2021

Vet World

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“…Although, molecular methods are insufficiently convenient for species identifications because of high genetic similarity; the ultrastructure observation of primary cyst wall technique is considered to be one possible reliable method for identifying Sarcocystis spp (6,7). Based on the reports from different provinces of Iran, infection rate of sarcocystosis in adult bovine muscles is absolutely high (8)(9)(10)(11)(12)(13)(14)(15), but there is no information about intensity of this parasitic disease in Sistan region. So, the present research aims to determine the intensity of infection and histopathological investigation of Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

Histopathological Survey on <i>Sarcocystis</i> Species Infection in Slaughtered Cattle of Zabol-Iran

Faghiri¹,

Davari²,

Nabavi³

2019

Turkiye Parazitol Derg

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Amaç: Sarkosistozis, dünya çapında yaygın bir dağılıma sahip ve çok çeşitli konakları olan önemli bir zoonotik protozoal hastalıktır. Bu çalışmanın amacı; Zabol-İran'da kesilen sığırlarda Sarkosistis spp. enfeksiyonunun yoğunluğunu belirlemek ve sarkosistis kistik lezyonlarının histopatolojik özellikleri göstermektir. Yöntemler: Nisan-Eylül 2018 tarihleri arasında kesilen 100 sığırın özofagus, kalp, diyafram, dil ve çiğneme kaslarından 500 doku örneği hazırlanmıştır. Tüm numuneler %10 nötr tamponlu formalin ile sabitlenmiştir ve tüm numunelere rutin doku işleme protokolü uygulanmıştır. Bulgular: Mikroskobik sonuçlar, örneklerin %92,2'sinde S. cruzi'nin ince duvarlı kistleri, %14'ünde kalın duvarlı Sarkosistis (S. hirsuta ve S. hominis) olduğunu, ancak makrokistin sadece bir sığırda görüldüğünü göstermiştir. İnce duvarlı kistlerin pozitiflik oranı kalp için %58,8, çiğneme kasları için %13,9, dil için %10,2, özofagus için %9,3 ve diyafram için %7,8 idi. S. hominis ve S. hirsuta'yı temsil edecek şekilde kalın duvarlı kistlerin pozitiflik oranı özofagus için %32,8, dil için %28,6, kalp için %22,9, çiğneme kasları için %15,7 ve diyafram için %0 idi. En çok enfekte olan doku kalp ve en az enfekte olan doku diyaframdı. İnce duvarlı kistler (S. cruzi) çoğunlukla kalpte bulundu ve diyaframda en az bulundu. Ancak, özofagusta kalın duvarlı kistler (S. hirsuta ve S. hominis) tespit edildi. Diyafram kasında kalın duvarlı kist bulunmadı. Sonuç: Zabol mezbahasında kesilen sığırlarda sarkosistosisin yüksek pozitiflik oranı, Sistan bölgesinin bu önemli paraziter hastalıkla ağır çevresel kontaminasyonunu ortaya çıkarmaktadır.

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“…In the present study, 97% of the heart samples were found to be positive for Sarcocystis and were identified as S. cruzi (100%). These findings should be considered by the veterinary organization, because S. cruzi, despite having no pathologic effects on humans, has severe effects on the livestock and can lead to severe clinical sign, abortion, weight loss, neurologic sign, fever, loss of animal products and death ( 23 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle

Ghaffari¹,

Dalimi²,

Pirestani³

2022

IJPA

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Background: Sarcocystis is a genus of coccidian protozoa that at least seven species of it can parasitize cattle. The global prevalence of Sarcocystis is close to 100% in adult cattle. The main aim of this study was to identify the infection rate of Sarcocystis spp. in heart of cattle in Tehran, Iran by microscopy and PCR-RFLP methods. Methods: Totally, 100 bovine heart samples were collected from the main slaughterhouse of Shahriar, Meysam slaughterhouse, west of Tehran in 2016. At first, heart samples were completely examined for the presence of sarcocystic macrocysts. Then, for microscopic examination, 50 g of each heart was digested in sterile condition using pepsin acid digestion method. Then, the species of the parasite were detected by PCR-RFLP technique and sequencing. Results: Overall, 97 of 100 of the heart muscle samples were infected with Sarcocystis. All the samples were detected as S. cruzi through similar patterns in PCR-RFLP. Conclusion: S. cruzi is the most common species in the heart of cattle slaughtered in Shahriar.

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Molecular Identification of Sarcocystis spp. in Sheep and Cattle by PCR-RFLP from Southwest of Iran

Cited by 13 publications

References 23 publications

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Investigation of Sarcocystis spp. in slaughtered cattle and sheep by peptic digestion and histological examination in Sulaimani Province, Iraq

Histopathological Survey on <i>Sarcocystis</i> Species Infection in Slaughtered Cattle of Zabol-Iran

Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle

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