Molecular identification of protozoal and bacterial organisms in domestic animals and their infesting ticks from north-eastern Algeria

Sadeddine, Rima; Diarra, Adama Zan; Laroche, Maureen; Mediannikov, Oleg; Righi, Souad; Benakhla, Ahmed; Dahmana, Handi; Raoult, Didier; Parola, Philippe

doi:10.1016/j.ttbdis.2019.101330

Cited by 31 publications

(27 citation statements)

References 48 publications

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“…Among these, the thermophilic species R. bursa, H. detritum, and H. marginatum predominated, and the mesophilic species I. ricinus was less abundant (Figure 1). These results corroborate the previous reports from northern Algeria [7,11,16]. In future studies, a combination of morphological and molecular identification of ticks should be performed to identify ticks at the genus level.…”

Section: Discussionsupporting

confidence: 91%

“…ticks [33], in our study, R. bursa, H. detritum, H. marginatum, and I. ricinus ticks were found to be positive for this pathogen. The association of this pathogen with R. bursa and R. annulatus ticks have also been confirmed in Romania and Algeria, respectively [11,34]. Moreover, a strain closely related to T. buffeli has been detected in R. sanguineus, R. bursa, R. annulatus, H. marginatum, Dermacentor marginatum, and Haemaphysalis punctata ticks in Sardinia, Italy [35].…”

Section: Discussionmentioning

confidence: 78%

“…In future studies, a combination of morphological and molecular identification of ticks should be performed to identify ticks at the genus level. In North Africa, TBP detection is usually carried out using classic methods, such as PCR or real-time PCRs, which are based on the use of specific primers and/or probes [11,17,18]. This approach is limited by the characterization of a single pathogen.…”

Section: Discussionmentioning

confidence: 99%

“…Hard ticks belonging to the genera Hyalomma, Rhipicephalus, Ixodes, and Haemaphysalis have been identified feeding on grazing cattle in Algeria [7]. In addition, various studies using classical molecular techniques have detected multiple pathogens in these ticks, e.g., the genera Rickettsia, Anaplasma, Coxiella, and Theileria, in cattle ticks from Algeria [8][9][10][11]. All of these studies used classical molecular methods that can only detect a few pathogens simultaneously, are time-consuming, and require large volumes of DNA for the detection of multiple pathogens.…”

Section: Introductionmentioning

confidence: 99%

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High-Throughput Microfluidic Real-Time PCR for the Detection of Multiple Microorganisms in Ixodid Cattle Ticks in Northeast Algeria

et al. 2021

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Ixodid ticks are hematophagous arthropods considered to be prominent ectoparasite vectors that have a negative impact on cattle, either through direct injury or via the transmission of several pathogens. In this study, we investigated the molecular infection rates of numerous tick-borne pathogens in ticks sampled on cattle from the Kabylia region, northeastern Algeria, using a high-throughput microfluidic real-time PCR system. A total of 235 ticks belonging to seven species of the genera Rhipicephalus, Hyalomma, and Ixodes were sampled on cattle and then screened for the presence of 36 different species of bacteria and protozoans. The most prevalent tick-borne microorganisms were Rickettsia spp. at 79.1%, followed by Francisella-like endosymbionts (62.9%), Theileria spp. (17.8%), Anaplasma spp. (14.4%), Bartonella spp. (6.8%), Borrelia spp. (6.8%), and Babesia spp. (2.5%). Among the 80.4% of ticks bearing microorganisms, 20%, 36.6%, 21.7%, and 2.1% were positive for one, two, three, and four different microorganisms, respectively. Rickettsia aeschlimannii was detected in Hyalomma marginatum, Hyalomma detritum, and Rhipicephalus bursa ticks. Rickettsia massiliae was found in Rhipicephalus sanguineus, and Rickettsiamonacensis and Rickettsia helvetica were detected in Ixodesricinus. Anaplasma marginale was found in all identified tick genera, but Anaplasma centrale was detected exclusively in Rhipicephalus spp. ticks. The DNA of Borrelia spp. and Bartonella spp. was identified in several tick species. Theileria orientalis was found in R. bursa, R. sanguineus, H. detritum, H. marginatum, and I. ricinus and Babesia bigemina was found in Rhipicephalus annulatus and R. sanguineus. Our study highlights the importance of tick-borne pathogens in cattle in Algeria.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High-Throughput Microfluidic Real-Time PCR for the Detection of Multiple Microorganisms in Ixodid Cattle Ticks in Northeast Algeria

et al. 2021

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“…The complete list of GenBank accession numbers of sequences from this study is shown in Table S3. Tick-borne pathogens (TBPs) of ovines represent a serious threat to veterinary and public health worldwide (16)(17)(18). In this study, three species of Anaplasma and two species of Babesia and Theileria were molecularly detected from sheep.…”

Section: Resultsmentioning

confidence: 93%

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Galon

Guo

et al. 2020

Front. Vet. Sci.

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Babesia, Theileria, and Anaplasma are important causative agents of tick-borne diseases that severely affect sheep. However, there is paucity in the occurrence genetic diversity of the infections of tick-borne diseases in sheep in border regions, northwestern China. In this study, nested polymerase chain reaction (nPCR) assays and gene sequencing were used to identify tick-borne Babesia spp., Theileria spp., and Anaplasma spp. infections in border regions, northwestern China. Out of 323 samples tested in this study, 225 (69.7%) sheep were infected with Babesia spp., Theileria spp., and Anaplasma spp. Two hundred six (63.8%), 60 (18.6%), 54 (16.7%), 51 (15.8%), 32 (9.9%), 19 (5.9%), and 16 (5.0%) were positive for A. ovis, B. motasi-like, A. bovis, T. uilenbergi, A. phagocytophilum, T. luwenshuni, and B. motasi-like Xinjiang, respectively. The most common dual infection was with A. ovis and B. motasi-like while the most frequent triple coinfection was A. ovis, B. motasi-like, and T. uilenbergi with coinfection rates of 17.0% (55/323) and 5.0% (16/323), respectively. Sequencing analysis indicated that A. ovis MSP4, A. phagocytophilum epank1, A. bovis 16S rRNA, B. motasi-like rap1-b, B. motasi-like Xinjiang rap1-a, T. luwenshuni 18S rRNA, and T. uilenbergi 18S rRNA from border regions, northwestern China, showed 99-100% identity with documented isolates from other countries. To the best of the authors' knowledge, this is the first report of T. uilenbergi and T. luwenshuni infections of sheep in border regions, northwestern China. Furthermore, these findings provide important data for understanding the distribution of Babesia, Theileria, and Anaplasma in sheep between border countries and China.

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Infection by haemopathogens and tick infestation of sheep during summer season in Constantine region, Northeast Algeria

Foughali

Jedidi

Dhibi

et al. 2021

Veterinary Medicine & Sci

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A study on tick infestation of 43 sheep with clinical symptoms of piroplasmosis and anaplasmosis was carried out during summer seasons of 2016 and 2017 in 34 sheep flocks from Beni Hamidene locality, district of Constantine, Northeast Algeria. Only animals with clinical symptoms of piroplasmosis and/or anaplasmosis were checked for tick infestation. Among the 43 examined sheep, 58 ± 15% were infested by ticks. A total of 185 adult ticks (100 males and 85 females) were collected from the 25 sheep. Two tick genera, Rhipicephalus and Hyalomma consisting of four species were collected, Rhipicephalus bursa was the most frequent tick (164/185; 88.6 ± 4.6%), followed by Rhipicephalus sanguineus sensu lato (16/185; 8.6 ± 4%), Rhipicephalus (Boophilus) annulatus (4/185; 2.2 ± 2.1%) and Hyalomma scupense (1/185; 0.5 ± 1%). Mean overall tick infestation intensity was 7.4. Mixed infestations with two tick species were found in 10 sheep (23.3 ± 12.6%). All farmers (34/34) used ivermectin, and only 11% of them used acaricide to control ticks. The majority of R. bursa ticks (87/185; 45 ± 7%) were located on the ears 37/185; 20 ± 6%) and the testicles (50/185; 27 ± 6%). Giemsa‐stained blood smears examination showed the presence of three haemopathogens: Anaplasma spp. (19/43; 44 ± 15%); Babesia spp. (6/43; 14 ± 10%) and Theileria spp. (1/43; 2 ± 4%). These results showed the presence of several low infestation burdens by ticks transmitting three haemopathogens.

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Molecular identification of protozoal and bacterial organisms in domestic animals and their infesting ticks from north-eastern Algeria

Cited by 31 publications

References 48 publications

High-Throughput Microfluidic Real-Time PCR for the Detection of Multiple Microorganisms in Ixodid Cattle Ticks in Northeast Algeria

High-Throughput Microfluidic Real-Time PCR for the Detection of Multiple Microorganisms in Ixodid Cattle Ticks in Northeast Algeria

Molecular Detection and Identification of Babesia spp., Theileria spp., and Anaplasma spp. in Sheep From Border Regions, Northwestern China

Infection by haemopathogens and tick infestation of sheep during summer season in Constantine region, Northeast Algeria

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