MOLECULAR IDENTIFICATION OF Pepper yellow leaf curl Indonesia virus ON CHILI PEPPER IN NUSA PENIDA ISLAND

Selangga, ‪Dewa Gede Wiryangga; Listihani, Listihani

doi:10.23960/jhptt.22197-102

Cited by 6 publications

(6 citation statements)

References 13 publications

(15 reference statements)

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“…The farmers in Sekaan Village, Bangli, Bali, Indonesia, implemented monoculture and intercropping planting patterns. The incidence and severity of the disease are found to be more severe in plantations with monoculture planting patterns compared to the intercropping pattern (Selangga et al 2021). According to Selangga and Listihani (2021), intercropping patterns with chili pepper among other crops from different families within a plantation and the use of resistant plant cultivars can lower disease infection levels and show high production yield in the field.…”

Section: Resultsmentioning

confidence: 99%

“…Begomovirus has been reported in Indonesia in tomato, eggplant, cucumber, pumpkin and sweet potato . In Indonesia, Begomovirus infection in chili pepper was first reported in Java and several years later Selangga et al (2019), Selangga and Listihani (2021), reported that chili pepper crops in several regions in (Bali Province) had been infected by Begomovirus. The main type of Begomovirus in chili pepper is Pepper yellow leaf curl virus (PYLCV).…”

Section: Introductionmentioning

confidence: 99%

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Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper

Temaja

SELANGGA²,

PHABIOLA³

et al. 2022

Biodiversitas

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Abstract. Temaja IGRM, Selangga DGW, Phabiola TA, Khalimi K, Listihani L. 2022. Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper. Biodiversitas 23: 5360-5366. Bemisia tabaci Genn is an important pest in horticulture crops. Until today, B. tabaci has always been found in chili pepper and causes damage. In addition to a direct injury to the chili pepper, it is also the vector of Begomovirus. There is no research till date regarding the relationship between B. tabaci with Begomovirus which caused damage to chili pepper crops. Thus, the aim of this research was to analyze the interaction between B. tabaci population with the disease incidence of Begomovirus on chili peppers crops, as well as the percentage of viruliferous B. tabaci. This research noted B. tabaci population dynamics, disease incidence of pepper yellow leaf curl disease, and the percentage of viruliferous B. tabaci via PCR by using Begomovirus universal primer and DNA analysis confirmation. The highest imago population occurred at 6 WAP with the average density 27.21 imago/yellow sticky card, while the highest nymph population occurred at 8 WAP with the average density of 4.26 nymph/leaf. The high population of B. tabaci nymph and imago caused the incidence of Begomovirus disease to reach 83.07% at 12 WAP. Result showed that high population of B. tabaci accelerated the speed of virus in the field. The confirmation result of Begomovirus in B. tabaci body showed that the virus was Pepper yellow leaf curl Indonesia virus (PYLCIV). The highest homology obtained was between PYLCIV isolates from Sekaan, Bangli with isolates from Bayung Gede, Bangli which ranged from 99.6 to 99.8%. Further phylogenetic analysis showed that PYLCIV isolates from Sekaan, Bali formed a group with the isolate from Bayung Gede, Bangli, which was from a different group with other isolates. This proved that B. tabaci carries and transmits PYLCIV to chili peppers in Bali.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper

Temaja

SELANGGA²,

PHABIOLA³

et al. 2022

Biodiversitas

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“…This showed that the whitefly vector was the main cause of begomovirus transmission in the field [ 38 ]. The incidence and severity of the disease were found to be more severe in plantations with monoculture planting patterns compared to those with the intercropping pattern [ 15 ]. The interaction synergy among species of begomovirus that infects pepper has been reported to be the cause of the breakdown of the natural defense system of pepper plants [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…In the field, symptoms of begomovirus infection on pepper are very typical such as chlorosis, mosaic, vein clearing, and bleaching [ 13 ]. Viral infections such as yellow curly leaves on pepper plants have spread rapidly into central pepper plants in Yogyakarta, Bali, and Nusa Penida [ 14 , 15 , 16 ]. The emergence of the first infection of Begomovirus disease in Indonesia is supported by evidence of B. tabaci mainly to two species of the complex, MEAM1 (Middle East–Asia Minor I, formerly referred to as biotype B) and Med (Mediterranean, formerly referred to as biotype Q), species that are highly invasive, highly polyphagous, and that transmit viruses on various crop [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Current Status and Complexity of Three Begomovirus Species in Pepper Plants in Lowlands and Highlands in Java Island, Indonesia

Wahyono

Murti

Hartono

et al. 2023

Viruses

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Three primary species from the Begomovirus genus, Pepper yellow leaf curl Indonesia virus (PepYLCIV), Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), and Tomato leaf curl New Delhi virus (ToLCNDV), are suspected of spreading throughout pepper production centers, and plants are infected by a single species or a combination of two or three species. This study was conducted to provide complete information about the symptoms, incidence and severity, whitefly biotypes, as well as the dominance status of the three Begomovirus species in pepper-producing areas in Java. A DNA analysis was carried out on leaf samples to identify Begomovirus species and biotypes of B. tabaci collected from 18 areas (16 districts) in lowlands (<400 m asl) and highlands (>700 m asl). The DNA analysis showed that B. tabaci biotype B was the most commonly detected in all locations compared to the A, AN, and Q biotypes. The incidence of begomovirus infection was at a high level, 93% and 88.78% in the lowlands and highlands, respectively. However, the severity of begomovirus infection was significantly higher in the lowlands (54.50%) than in the highlands (38.11%). A single infection of PepYLCIV was most dominant in all locations sampled and caused severe infection, followed by a mixed infection with TYLCKaV. Therefore, the current status of begomovirus infection, especially PepYLCIV, can provide advice to farmers using more tolerant and resistant varieties as well as a breeding strategy for resistant pepper varieties.

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“…Begomovirus is transmitted by whitefly, Bemisia tabaci Genn., a sapsucking insect belonging to family Aleyrodidae. The disease can cause loss up to 90% on legumes, cucurbits, the Solanaceae, cassava, papaya and cottons [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of gamma ray induced mutations in PepYLCV-infecting local pepper in Karo Regency, Sumatra Utara, Indonesia

Tarigan

Hanafiah²,

Sinuraya³

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Plant mutation breeding plays a pivotal role in increasing plant genetic variability, pest and disease resistance and yield production. Gamma ray irradiation induced to infected plants to obtain resistant mutant plants. This research aims to examine the morphological characterization of pepper plants and the mutant plants produced from irradiated PepYLCV-infecting local pepper from Karo Regency, Sumatra Utara, Indonesia. The research was conducted in January to October 2021 in farming land in Tongkoh Village, Dolat Rayat District, Karo Regency, Sumatra Utara, using non-Factorial Randomized Block Design. The mutation was induced by exposing the seeds of local chili to varied doses (D): 0 (uninfected seeds and unirradiated infected seeds), 150, 200 and 250 Gy and was performed using T-Test, replicated 3 times. The results of research showed that different doses of gamma rays significantly affected the mutant plants from the observation on its plant height, stem diameter, canopy and fruit lengths, fruit weight, number of branches, leaves and fruits. Plant induced with gamma ray 150 Gy found to have the longest canopy and fruit and also demonstrated the highest number of branches, flowers, leaves and the highest fruit weight compared to other higher doses. The exposure of gamma irradiation gave positive effect to infected chili to enhance their resistance in order to produce higher yield.

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MOLECULAR IDENTIFICATION OF Pepper yellow leaf curl Indonesia virus ON CHILI PEPPER IN NUSA PENIDA ISLAND

Cited by 6 publications

References 13 publications

Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper

Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper

Current Status and Complexity of Three Begomovirus Species in Pepper Plants in Lowlands and Highlands in Java Island, Indonesia

Characterization of gamma ray induced mutations in PepYLCV-infecting local pepper in Karo Regency, Sumatra Utara, Indonesia

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