Molecular identification of oomycete species affecting aquaculture in Bangladesh

Sarowar, Mohammad Nasif; Hossain, Jakir; Nasrin, Tahmina; Naznin, Tania; Hossain, Zakir; Rahman, Mohammad Matiur

doi:10.1016/j.aaf.2018.12.003

Cited by 10 publications

(8 citation statements)

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“…were reported from several teleosts in Poland but the isolates were not pathogenic (Czeczuga, Kiziewicz, & Danilkiewicz, ). In addition, more than one species can cohabit in a culture plate which can result in difficulty in identification through DNA sequencing (Sarowar et al, ). Developing a single‐spore culture (SSC) from the collected samples can be very useful to avoid aforementioned problems of plate culture.…”

Section: Discussionmentioning

confidence: 99%

“…This may explain the few cases of saprolegniosis in rainbow trout (Fadaeifard, Raissy, Bahrami, Rahimi, & Najafipoor, 2011;Fregeneda Grandes, Fernández Díez, & Aller Gancedo, 2001;Gieseker, Serfling, & Reimschuessel, 2006 (Czeczuga, Kiziewicz, & Danilkiewicz, 2002). In addition, more than one species can cohabit in a culture plate which can result in difficulty in identification through DNA sequencing (Sarowar et al, 2019). Developing a single-spore culture (SSC) from the collected samples can be very useful to avoid aforementioned problems of plate culture.…”

Section: Discussionmentioning

confidence: 99%

“…The Cox1 and Cox2 regions were amplified using primer pairs developed by Hudspeth, Nadler, and Hudspeth (2000) and Ravasi, De Respinis, and Wahli (2018). All PCRs were prepared and run according to the protocol described by Sarowar et al (2019). The amplified PCR products were cleaned up by Wizard ® SV Gel and PCR Clean-Up System (Promega) and then processed by Sanger sequencing methods using capillary-based fluorescent sequencing on an automated unit (ABI 3730XL DNA, USA; The Centre for Applied Genomics, SickKids ® ).…”

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

“…Typical disease signs are visible white and grey cotton-wool like patches of filamentous mycelia growing on the skin and gills (Stueland, Hatai, & Skaar, 2005). Among the several species of Saprolegnia, S. parasitica poses the greatest threat to fish and is now considered a specialized pathogen, and not a saprotroph (Hussein & Hatai, 2002;Sandoval-Sierra, Latif-Eugenin, et al, 2014;Sarowar et al, 2019). It produces special "boat hooks" on its secondary cysts to aid attachment to hosts (Rezinciuc, Sandoval-Sierra, Ruiz-León, van West, & Diéguez-Uribeondo, 2018), and the mycelia form "appressoria" to gain access through teleost skin/eggs to establish infection (Willoughby & Hasenjäger, 1987), and can suppress the host immune response by translocating specific proteins into host cells (Belmonte et al, 2014;Trusch et al, 2018;Wawra et al, 2012;van West et al, 2010).…”

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confidence: 99%

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Saprolegnia molecular phylogeny among farmed teleosts in Nova Scotia, Canada

Sarowar

Cusack

Duston

2019

Journal of Fish Diseases

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To identify the pathogens causing saprolegniosis among farmed fish in Nova Scotia, 172 infected tissues and 23 water samples were collected from six species of teleosts: Atlantic salmon (Salmo salar), brown trout (Salmo trutta), Arctic charr (Salvelinus alpinus), brook trout (Salvelinus fontinalis), striped bass (Morone saxatilis) and rainbow trout (Oncorhynchus mykiss) at nine facilities over a 600 km range. Following laboratory culture, 132 isolates were recovered. Six species of oomycetes were identified from analysis of the internal transcribed spacer (ITS) sequence of the nrDNA: Saprolegnia parasitica, Saprolegnia ferax, Saprolegnia diclina, Saprolegnia aenigmatica, Saprolegnia torulosa, Saprolegnia sp. and Pythiopsis cymosa. Further phylogenetic analyses of the ITS and cytochrome c oxidase subunit 1 (Cox1) regions revealed four strains of Saprolegnia parasitica (named here as S1, S2, S3 and S4), of which S1 and S2 were common (37% and 42% of the isolates), and two strains of S. ferax. Among S. parasitica, S2 and S3 are more closely related to each other than to S1 based on the phylogenetic analyses and predicted RNA secondary structure of the ITS region. Sexual structures with a similar morphology were formed by S1 and S3 in vitro, but were not formed by S2.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

mentioning

confidence: 99%

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Saprolegnia molecular phylogeny among farmed teleosts in Nova Scotia, Canada

Sarowar

Cusack

Duston

2019

Journal of Fish Diseases

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“…Saprolegniosis has significant impact on freshwater ecosystems by infecting different host organisms and their eggs (Kiesecker et al, 2001;Fernández-Benéitez et al 2008;Ruthig 2009;Eissa et al, 2013). Some species of Aphanomyces invadans are regularly in charge of serious disease outbreaks in fish farms and in the common habitat causing enormous economic losses by threatening industry generation targets and long-term viability (Sarowar et al, 2013;Van West and Beakes, 2014;Iberahim et al, 2018;Sarowar et al, 2019). The World Organization for Animal Health (OIE) listed that this disease has been responsible for large-scale mortalities of farmed and wild fish in more than 20 countries across four continents (Pradhan et al, 2008;Iberahim et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Insight into Isolation, Identification and Antimicrobial Sensitivity of some Moulds Isolated from Fresh Water Fishes

Tawab¹,

Hofy²,

Moustafa³

et al. 2020

Adv. Anim. Vet. Sci.

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| The expanding recurrence of invasive fungal infection and the high mortality associated with dispersed fungal disease have highlighted the requirement for fast recognizable proof of infectious molds. The current study was conducted to identify the most prevalent mycotic diseases among cultured freshwater fishes with special focus on isolation, molecular identification and antimicrobial sensitivity testing. A total number of 300 examined cultured freshwater fishes; 200 Oreochromis niloticus (120 apparently healthy and 80 showing clinical signs) and 100 Clarias gariepinus (30 apparently healthy and 70 showing clinical signs), were collected alive from five freshwater fish farms at Kafr El Sheikh Governorate all over the year 2018. Clinically, examined Oreochromis niloticus revealed scale detachment, fins erosion, pale body coloration and eye opacity particularly in fish naturally infected with Aphanomycess, Aspergillus, Pencillium and Fusarium; however, infected Clarias gariepinus showed scattered hemorrhagic patches on the ventral abdomen. Mycological examination revealed the isolation of 900 fungal isolates; 570 fungal isolates from Oreochromis niloticus and 330 isolates from C. gariepinus. Aphanomyces invadans was the predominant fungi among diseased fishes mainly from skin, fins and gills. However, Aspergillus flavus, Aspergillus niger and Alternaria sp. were the most predominant fungi isolated from apparently healthy fishes mainly from internal organs. Molecular identification was performed using inter-transcriped spacer (ITS) gene for the most prevalent isolates among either diseased or apparently healthy fishes. Developed PCR assay specific primer detect not only Aphanomyces invadans showing clear bands at 569 bp molecular weight, but also Asperigillus flavus and Asperigillus niger showing clear bands at 595 and 600 bp molecular weight, respectively. Anti-fungal sensitivity test revealed that all mould isolates are sensitive to Nystatin and the majority was resistant to Voriconazole. Briefly, being normal mycoflora, most fungi still can cause infections to fishes. Consequently, proper health management practices should be adapted while fish rearing.Citation | El-Tawab AAA, El-Hofy FI, Moustafa EM, Halawa MR (2020). Insight into isolation, identification and antimicrobial sensitivity of some moulds isolated from fresh water fishes. Adv. Anim. Vet. Sci. 8(2): 174-182.

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