Abstract:This work was involved DNA extraction from cultured Brachionus calyciflorus subjected to PCR .The PCR reaction was performed with Brachionus calyciflorus which having identical sequences of 18S rDNA and recorded as a global isolates of Brachionus calyciflorus in the (NCBI of USA, ENA of Europe and DDBJ of Japan) with Genbank ID: KM191796.1.
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