Molecular identification of Mycobacterium avium subspecies paratuberculosis in oral biopsies of Crohn’s disease patients

Molicotti, Paola; Scanu, Antonio Mario; Lumbau, A; Cannas, Sara; Bua, Alessandra; Lugliè, Pietrina Francesca; Zanetti, Stefania Anna Lucia

doi:10.1186/1757-4749-5-18

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…It is found with similar histopathological findings in humans to those observed commonly in JD of ruminants (Uzoigwe et al ., ). A number of scientific evidences in support of causal association between Crohn's disease and MAP have been reported in the literature (Molicotti et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Proteome-wide B and T cell epitope repertoires in outer membrane proteins ofMycobacterium aviumsubsp.paratuberculosishave vaccine and diagnostic relevance: a holistic approach

2015

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Mycobacterium avium subsp. paratuberculosis (MAP) is an etiological agent of chronic inflammation of the intestine among ruminants and humans. Currently, there are no effective vaccines and sensitive diagnostic tests available for its control and detection. For this, it is of paramount importance to identify the MAP antigens, which may be immunologically recognized by the host immune system. To address this challenge, we performed identification of the immunogenic epitopes in the MAP outer membrane proteins (OMPs). We have previously identified 57 MAP proteins as OMPs [Rana A, Rub A, Akhter Y. 2014. Molecular BioSystems, 10:2329-2337] and have evaluated them for the epitope selection and analysis employing a computational approach. Thirty-five MAP OMPs are reported with nine-mer peptides showing high binding affinity to major histocompatibility complex (MHC) class I molecules and 28 MAP OMPs with 15-mer peptides of high binding affinity for MHC class II molecules. The presence of MHC binding epitopes indicates the potential cell-mediated immune response inducing capacity of these MAP OMPs in infected host. To further investigate the humoral response inducing properties of OMPs of MAP, we report potential B cell epitopes based on the sequences of peptide antigens and their molecular structures. We also report 10 proteins having epitopes for both B and T cells representing potential candidates which may invoke both humoral and cellular immune responses in the host. These findings will greatly accelerate and expedite the formulation of effective and cost-efficient vaccines and diagnostic tests against MAP infection.

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Section: Discussionmentioning

confidence: 97%

Proteome-wide B and T cell epitope repertoires in outer membrane proteins ofMycobacterium aviumsubsp.paratuberculosishave vaccine and diagnostic relevance: a holistic approach

2015

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“…Important members of this group are M. avium subsp. paratuberculosis (Map), the pathogenic agent of Johne's disease in ruminants that has also been isolated from human Crohn's disease patients [ 4 ], and M. avium subsp. avium (Maa).…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium aviumSubsp.aviumInfection in Four Veal Calves: Differentiation from Intestinal Tuberculosis

Goepfert

Stokar-Regenscheit

Schumacher

et al. 2014

BioMed Research International

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Mycobacterium avium subsp. avium (Maa) is an intracellular pathogen belonging to the Mycobacterium avium-intracellulare complex (MAC). Reservoirs of MAC are the natural environment, wildlife and domestic animals. In adult bovine, MAC infections are typically caused by Mycobacterium avium subsp. paratuberculosis (Map). Maa infections in bovine are rarely reported but may cause clinical disease and pathological lesions similar to those observed in paratuberculosis or those induced by members of the Mycobacterium tuberculosis complex (MTBC). Therefore, differentiation of MAC from MTBC infection should be attempted, especially if unusual mycobacterial lesions are encountered. Four veal calves from a fattening farm dying with clinical signs of otitis media, fever, and weight loss were submitted for necropsy. Samples from affected organs were taken for histologic investigation, bacteriologic culture, and bacterial specification using PCR. Macroscopic thickening of the intestinal mucosa was induced by granulomatous enteritis and colitis. Intracytoplasmic acid-fast bacteria were detected by Ziehl-Neelsen stains and PCR revealed positive results for Mycobacterium avium subsp. avium. Clinical and pathological changes of Maa infection in veal calves had features of Mycobacterium avium subsp. paratuberculosis and the MTBC. Therefore, Mycobacterium tuberculosis complex infection should be considered in cases of granulomatous enteritis in calves.

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Evaluation of the humoral response against mycobacterial peptides, homologous to MOG35–55, in multiple sclerosis patients

Cossu

Mameli

Masala

et al. 2014

Journal of the Neurological Sciences

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Molecular identification of Mycobacterium avium subspecies paratuberculosis in oral biopsies of Crohn’s disease patients

Cited by 3 publications

References 12 publications

Proteome-wide B and T cell epitope repertoires in outer membrane proteins ofMycobacterium aviumsubsp.paratuberculosishave vaccine and diagnostic relevance: a holistic approach

Proteome-wide B and T cell epitope repertoires in outer membrane proteins ofMycobacterium aviumsubsp.paratuberculosishave vaccine and diagnostic relevance: a holistic approach

Mycobacterium aviumSubsp.aviumInfection in Four Veal Calves: Differentiation from Intestinal Tuberculosis

Evaluation of the humoral response against mycobacterial peptides, homologous to MOG35–55, in multiple sclerosis patients

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