Molecular Identification of Lipase Producing Bacteria based on 16S rDNA Sequencing

Pallavi, P.; Bhavani, P.; Komali, J.; Manjusha, T.

doi:10.20546/ijcmas.2017.605.230

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F Determination Of Protein Content and Lipase Activity1

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2018

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“…Bradford"s assay was used to determine the total protein concentrations [9]. Enzyme activity was determined using the lipase assay methods previously used by [10], [11], [12], [13].…”

Section: F Determination Of Protein Content and Lipase Activitymentioning

confidence: 99%

Microbial Screening for Bio-delipidation System of Pre-treated Poultry Slaughterhouse Wastewater: Impact of Solvents, Metal Ions and Detergents on Lipase Activity

Mbulawa¹,

Ntwampe²,

Basitere³

et al. 2018

ASETH-18,ACABES-18 &Amp; EBHSSS-18 Nov. 19-20 2018 Cape Town (South Africa)

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This study reports on the microbial screening for a bio-delipidation system of lipid-rich slaughterhouse wastewater, and on the optimal conditions for lipase production and activity. In this study, swaps were collected from the poultry slaughterhouse discharge point for screening, isolation and characterisation of lipolytic microorganisms using molecular techniques. Bacillus cereus strains AB1 (BF3) and CC-1 (B3O) were identified using 16S rRNA techniques. Maximal lipase production for both strains was observed between pH 6-8 and 45-60 °C. Optimal lipase activity for BF3 and B30 was achieved at pH 8 and 60 °C, and at pH 8.83 and 45°C, respectively. After partial purification, increased activity was observed for BF3 and B30 strains. Solvents, metal ions and detergents (triclosan and trichlorocarbonilide) affected lipase activity. It was concluded that BF3 and B30 strains were suitable candidates for bio-delipidation systems.

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“…Bradford"s assay was used to determine the total protein concentrations [9]. Enzyme activity was determined using the lipase assay methods previously used by [10], [11], [12], [13].…”

Section: F Determination Of Protein Content and Lipase Activitymentioning

confidence: 99%