Molecular identification of Leishmania RNA virus in cutaneous leishmaniasis patients and rodent reservoirs in Isfahan province, Iran

Farrokhi-Karibozorg, Mojgan; Ghayour-Najafabadi, Zahra; Hejazi‬, ‪Seyed Hossein; Ataei‐Pirkooh, Angila; Mohebali, Mehdi; Teimouri, Parvin; Hajjaran, Homa

doi:10.1016/j.meegid.2022.105222

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…Interestingly, their elimination from L. major and L. guyanensis in vitro prompts different cellular responses (Saura et al ., 2022 ). Our previous analyses of the available central Asian strains of L. gerbilli ( n = 2), L. major ( n = 14) and L. turanica ( n = 12) revealed the presence of LRV-2 only in L. major with the prevalence of about 65% (Kleschenko et al ., 2019 ; Kostygov et al ., 2021 a ) corroborating findings of LRV-2 in the same parasite species in Iran and Turkey (Hajjaran et al ., 2016 ; Kurt et al ., 2019 ; Nalçacı et al ., 2019 ; Saberi et al ., 2020 , 2022 ; Farrokhi-Karibozorg et al ., 2022 ; Moin-Vaziri et al ., 2022 ). The prevalence of L. major infection by LRV-2 in these studies varied between 2 and 68% and the presence of LRV-2 was confirmed in 87 out of 221 isolates of L. major analysed (only 1 isolate of L. turanica was included and reported as negative and no isolates of L. gerbilli were analysed).…”

Section: Discussionmentioning

confidence: 99%

Revisiting epidemiology of leishmaniasis in central Asia: lessons learnt

et al. 2022

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In this work we reviewed historical and recent data on Leishmania spp. infection combining data collected in Turkmenistan, Uzbekistan, Kazakhstan, Kyrgyzstan, Iran, China and Mongolia. We specifically focused on a complex of co-existing species (Leishmania major, Leishmania turanica and Leishmania gerbilli) sharing the same animal reservoirs and vectors. In addition, we analysed the presence of dsRNA viruses in these species and discussed future research directions to identify species-specific traits, which may determine susceptibility of different Leishmania spp. to viral infection.

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Section: Discussionmentioning

confidence: 99%

Revisiting epidemiology of leishmaniasis in central Asia: lessons learnt

et al. 2022

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“…We surveyed 59 samples of L. major and 1 L. tropica isolated from infected patients. But, LRV2 detection was done on 13 samples due to the cultivation failure; just 1 isolate of L. major was LRV2 positive, which is the first report of LRV2 detection in Aran o Bidgol and 5th in Iran [21,22,35,36].…”

Section: Discussionmentioning

confidence: 98%

Successful Isolation of Leishmania RNA Virus (LRV) from Leishmania major in a Cutaneous Leishmaniasis Focus in Central Iran: An Update on Cases

et al. 2022

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Purpose Cutaneous leishmaniasis (CL) is a major vector-borne disease that affects people globally, including Iran. Different factors are associated with leishmaniasis pathogenicity; recently, a link of the possible relationship between Leishmania RNA Virus (LRV) and disease severity was proposed, especially in the New World leishmaniasis (NWL). This study was aimed to investigate the presence of LRV2 in Leishmania isolates in Aran o Bidgol, Isfahan province. Methods Samples were collected from 110 CL-suspected patients referred to the health center. In this study, we aimed to investigate CL cases (parasitologically and clinically), identify Leishmania species (by ITS1-PCR-RFLP), and finally detection of LRV2 (by RdRp-semi-nested PCR). Results Parasitological methods showed 60 positive cases, based on the HaeIII enzyme restriction profile, 59 cases were caused by L. major and 1 case by L. tropica. Our project is the first study on LRV2 isolation in Aran o Bidgol city and the LRV was successfully detected from a single L. major isolated in a women's hand lesion. Using BLAST, 94.8-100% similarity was observed in the RdRp sequence of current LRV isolate with those available in GenBank from Iran or overseas. Conclusion L. major was the main cause of CL in Aran o Bidgol, although L. tropica is also present in a much lower proportion in the area. This is the first report on the presence of LRV2 in Aran o Bidgol and the fifth in Iran.

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“…The final extension of the strands consisted of 72 °C for 4 min. The PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with SYBR safe gel stain (Thermo Fisher Scientific, USA) next to the 100 bp DNA marker (Fermentas, Life Sciences) [ 17 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

The effects of Leishmania RNA virus 2 (LRV2) on the virulence factors of L. major and pro-inflammatory biomarkers: an in vitro study on human monocyte cell line (THP-1)

Mirabedini,

Mirjalali,

Kazemirad

et al. 2023

BMC Microbiol

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Background Cutaneous Leishmaniasis (CL) is a parasitic disease with diverse outcomes. Clinical diversity is influenced by various factors such as Leishmania species and host genetic background. The role of Leishmania RNA virus (LRV), as an endosymbiont, is suggested to not only affect the pathogenesis of Leishmania, but also impact host immune responses. This study aimed to investigate the influence of LRV2 on the expression of a number of virulence factors (VFs) of Leishmania and pro-inflammatory biomarkers. Materials and methods Sample were obtained from CL patients from Golestan province. Leishmania species were identified by PCR (LIN 4, 17), and the presence of LRV2 was checked using the semi-nested PCR (RdRp gene). Human monocyte cell line (THP-1) was treated with three isolates of L. major with LRV2 and one isolate of L. major without LRV2. The treatments with four isolates were administered for the time points: zero, 12, 24, 36, and 48 h after co-infection. The expression levels of Leishmania VFs genes including GP63, HSP83, and MPI, as well as pro-inflammatory biomarkers genes including NLRP3, IL18, and IL1β, were measured using quantitative real-time PCR. Results The expression of GP63, HSP83, and MPI revealed up-regulation in LRV2 + isolates compared to LRV2- isolates. The expression of the pro-inflammatory biomarkers including NLRP3, IL1β, and IL18 genes in LRV2- were higher than LRV2 + isolates. Conclusion This finding suggests that LRV2 + may have a probable effect on the Leishmania VFs and pro-inflammatory biomarkers in the human macrophage model.

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Molecular identification of Leishmania RNA virus in cutaneous leishmaniasis patients and rodent reservoirs in Isfahan province, Iran

Cited by 4 publications

References 31 publications

Revisiting epidemiology of leishmaniasis in central Asia: lessons learnt

Revisiting epidemiology of leishmaniasis in central Asia: lessons learnt

Successful Isolation of Leishmania RNA Virus (LRV) from Leishmania major in a Cutaneous Leishmaniasis Focus in Central Iran: An Update on Cases

The effects of Leishmania RNA virus 2 (LRV2) on the virulence factors of L. major and pro-inflammatory biomarkers: an in vitro study on human monocyte cell line (THP-1)

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