Molecular Identification of Invasive Non-typeable Group B Streptococcus Isolates From Denmark (2015 to 2017)

Slotved, Hans Christian; Fuursted, Kurt; Kavalari, Ioanna Drakaki; Hoffmann, Steen

doi:10.3389/fcimb.2021.571901

Cited by 7 publications

(7 citation statements)

References 34 publications

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“… 35 , 36 Recent advances in molecular techniques have enhanced the capabilities of epidemiological analysis of the origin of GBS isolates. 11 , 37 In this case, our results showed that the GBS isolates from the infant bloodstream and maternal breast milk had a high consistency in homology analysis. However, the GBS isolates from the mother’s perinatal cervical secretion exhibited differences in homology analysis.…”

Section: Discussionsupporting

confidence: 51%

Late-Onset Sepsis in a Premature Infant Mediated by Breast Milk: Mother-to-Infant Transmission of Group B Streptococcus Detected by Whole-Genome Sequencing

Li¹,

Fang²,

Hao³

et al. 2022

IDR

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Background Late-onset group B Streptococcus (LOGBS) sepsis is a cause of infection and death in infants. Infected breast milk has been considered a source of neonatal GBS infection and invasive infection. However, mother-to-infant transmission of GBS detected by the high-resolution diagnostic method is rarely reported. Methods This study describes a low-weight premature infant who developed late-onset GBS septicemia 21 days after birth. GBS strains isolated from the mother’s cervical secretion, the mother’s milk, and the baby’s blood were cultured to identify the source of GBS infection. We further confirmed the GBS isolates through matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Finally, we performed whole-genome sequencing (WGS) and phylogenetic analyses on the GBS strains recovered. Results GBS isolates were cultured from the bloodstream of the premature infant and the mother’s milk, respectively. Subsequently, WGS and phylogenetic analyses on three GBS isolates demonstrated that the GBS strain from the infant’s bloodstream was 100% homologous to that from the mother’s breast milk, which had some different gene fragments from the GBS strain from the mother’s cervical secretion. It provided evidence that this infant’s late-onset GBS septicemia originated from his mother’s breast milk instead of the vertical mother-to-infant transmission. Conclusion Through WGS and phylogenetic analysis of the GBS strains, we proved in this study that the late-onset GBS sepsis in a premature infant was derived from his mother’s breast milk. It indicated that WGS diagnosis is an effective tool for infection tracing. Furthermore, this report provides direction for preventing late-onset GBS infection.

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Section: Discussionsupporting

confidence: 51%

Late-Onset Sepsis in a Premature Infant Mediated by Breast Milk: Mother-to-Infant Transmission of Group B Streptococcus Detected by Whole-Genome Sequencing

Li¹,

Fang²,

Hao³

et al. 2022

IDR

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“…Twelve studies were conducted across the WHO European region, mainly in the Netherlands [13, 14], Denmark [15, 16] and Sweden [17, 18]. Likewise, six studies originated from the WHO Western Pacific [19–24] and Americas regions [7–11, 25, 26] ().…”

Section: Resultsmentioning

confidence: 99%

“…No study originated from the African, Eastern Mediterranean or Southeast Asian regions. Twelve studies [7, 14–16, 19, 21, 22, 25, 27–30] only investigated the genomic epidemiology of GBS in diseased adults, whereas the remaining considered GBS concomitantly in non-pregnant populations and neonates and/or pregnant women (8–11, 13, 17, 18, 20, 23, 26, 31–33; ). Overall, a total of 14 208 participants were described across the included studies, with a total of 14 110 GBS isolates being analysed.…”

Section: Resultsmentioning

confidence: 99%

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Molecular epidemiology of Streptococcus agalactiae in non-pregnant populations: a systematic review

Founou,

Khan,

Medugu

et al. 2023

Microbial Genomics

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Streptococcus agalactiae (group B Streptococcus , GBS) has recently emerged as an important pathogen among adults. However, it is overlooked in this population, with all global efforts being directed towards its containment among pregnant women and neonates. This systematic review assessed the molecular epidemiology and compared how the lineages circulating among non-pregnant populations relate to those of pregnant and neonatal populations worldwide. A systematic search was performed across nine databases from 1 January 2000 up to and including 20 September 2021, with no language restrictions. The Joanna Briggs Institute (JBI) Prevalence Critical Appraisal Tool (PCAT) was used to assess the quality of included studies. The global population structure of GBS from the non-pregnant population was analysed using in silico typing and phylogenetic reconstruction tools. Twenty-four articles out of 13 509 retrieved across 9 databases were eligible. Most studies were conducted in the World Health Organization European region (12/24, 50 %), followed by the Western Pacific region (6/24, 25 %) and the Americas region (6/24, 25 %). Serotype V (23%, 2310/10240) and clonal complex (CC) 1 (29 %, 2157/7470) were the most frequent serotype and CC, respectively. The pilus island PI1 : PI2A combination (29 %, 3931/13751) was the most prevalent surface protein gene, while the tetracycline resistance tetM (55 %, 5892/10624) was the leading antibiotic resistance gene. This study highlights that, given the common serotype distribution identified among non-pregnant populations (V, III, Ia, Ib, II and IV), vaccines including these six serotypes will provide broad coverage. The study indicates advanced molecular epidemiology studies, especially in resource-constrained settings for evidence-based decisions. Finally, the study shows that considering all at-risk populations in an inclusive approach is essential to ensure the sustainable containment of GBS.

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“…Whole-genome sequencing was performed as previously described ( Fuursted et al, 2016 ; Slotved et al, 2021 ). Briefly, genomic DNA was extracted using a DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany), and fragment libraries were constructed using a Nextera XT Kit (Illumina, San Diego, CA, USA) followed by either 150 or 250 bp paired-end sequencing on either the MiSeq or NextSeq 550 platform (Illumina, San Diego, CA, USA), respectively, according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of molecular typing for national surveillance of invasive clinical Haemophilus influenzae isolates from Denmark

et al. 2022

Self Cite

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Haemophilus influenzae is a gram-negative coccobacillus known to cause respiratory and invasive infections. It can possess a polysaccharide capsule that can be categorized into six different serotypes (i.e., Hia, Hib, Hic, Hid, Hie, and Hif) and non-encapsulated strains that are defined as non-typeable. Furthermore, H. influenzae can be characterized into eight biotypes (I–VIII). Traditionally, isolates have been serotyped and biotyped using phenotypic methods; however, these methods are not always reliable. In this study, we evaluate the use of whole-genome sequencing (WGS) for national surveillance and characterization of clinical Danish H. influenzae isolates. In Denmark, all clinical invasive isolates between 2014 and 2021 have been serotyped using a traditional phenotypic latex agglutination test as well as in silico serotyped using the in silico programs “hinfluenzae_capsule_characterization” and “hicap” to compare the subsequent serotypes. Moreover, isolates were also biotyped using a phenotypic enzyme test and the genomic data for the detection of the genes encoding ornithine, tryptophan, and urease. The results showed a 99–100% concordance between the two genotypic approaches and the phenotypic serotyping, respectively. The biotyping showed a 95% concordance between genotyping and phenotyping. In conclusion, our results show that in a clinical surveillance setting, in silico serotyping and WGS-based biotyping are a robust and reliable approach for typing clinical H. influenzae isolates.

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Molecular Identification of Invasive Non-typeable Group B Streptococcus Isolates From Denmark (2015 to 2017)

Cited by 7 publications

References 34 publications

Late-Onset Sepsis in a Premature Infant Mediated by Breast Milk: Mother-to-Infant Transmission of Group B Streptococcus Detected by Whole-Genome Sequencing

Late-Onset Sepsis in a Premature Infant Mediated by Breast Milk: Mother-to-Infant Transmission of Group B Streptococcus Detected by Whole-Genome Sequencing

Molecular epidemiology of Streptococcus agalactiae in non-pregnant populations: a systematic review

Evaluation of molecular typing for national surveillance of invasive clinical Haemophilus influenzae isolates from Denmark

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