Molecular Identification of <i>Spirometra erinaceieuropaei</i> Tapeworm in Cases of Human Sparganosis, Hong Kong

Tang, Tommy H.C.; Wong, Samson S. Y.; Lai, Christopher K C; Poon, Rosana W.S.; Chan, Helen S.Y.; Wu, Tak Chiu; Cheung, Yuk-fai; Poon, Tak‐Lap; Tsang, Yi‐Po; Tang, Wai-Lun; Wu, Alan K.L.

doi:10.3201/eid2304.160791

Cited by 22 publications

(18 citation statements)

References 14 publications

(11 reference statements)

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“…The major aetiological agent of human sparganosis is Spirometra erinaceieuropaei which is mainly acquired by the consumption of undercooked or uncooked meat of frogs and snakes (Anantaphruti et al ., 2011). Although S. erinaceieuropaei was distributed globally, most sparganosis cases occur in eastern and southeastern Asian countries (Almeida et al ., 2016; Tang et al ., 2017). China has the largest number of sparganosis cases in the world with more than 1000 cases have been reported, scattered over almost all administrative areas, so sparganosis still poses a serious threat to human health in China (Liu et al ., 2015).…”

Section: Introductionmentioning

confidence: 99%

Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis

Hong

Duan

et al. 2019

Parasitology

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The plerocercoid (sparganum) of Spirometra erinaceieuropaei is the main aetiological agent of human sparganosis. To improve the current knowledge on S. erinaceieuropaei evolution, we performed multi-locus microsatellite typing of sparganum isolates from China for the first time. All available expressed sequence tag (EST) sequences for the Spirometra were downloaded from the GenBank. The identification and localization of microsatellites in ESTs was accomplished by MISA. Based on the selected microsatellites, the genetic structure of 64 sparganum isolates collected from 11 geographical locations in southwest China were investigated through principal component analysis, STRUCTURE analysis and neighbour-joining clustering. A total of 522 non-redundant ESTs containing 915 simple sequence repeats were identified from 12 481 ESTs screened. Five primer pairs were finally selected. Using these loci, a total of 12 alleles were detected in 64 sparganum isolates. Little variability was observed within each of geographical population, especially among isolates derived from Kunming of Yunnan (YN-KM) province. Both STRUCTURE analysis and the clustering analysis supported that two genotypes existed among the sparganum isolates from southwest China. In conclusion, five microsatellite markers were successfully developed, and sparganum population was observed to harbour low genetic variation, further investigation with deeper sampling was needed to elucidate the population structure.

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Section: Introductionmentioning

confidence: 99%

Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis

Hong

Duan

et al. 2019

Parasitology

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“…A domestic retrospective genetic analysis and sequencing analysis of 9 cases of human sparganosis in Hong Kong. The results showed that the 9 samples could be confirmed as S. erinaceieuropaei by the cox 1 and 28S rRNA genes identification [17].…”

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confidence: 99%

Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China

Fang

Xue

et al. 2019

Korean J Parasitol

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Spargana were collected from human and frogs in Liaoning and Hubei Provinces, China. PCR amplification and direct sequencing of A cox1 fragment was PCR-amplified from genomic DNA extracted from 7 specimens (5 from humans and 2 from frogs). The cox1 fragment (390 bp) showed 97-100% similarity to the reference sequence of S. erinaceieuropaei and 88-89% to the reference sequence of S. decipiens. There were 1-12 bases different between these worms, but no obvious genetic variation (0-3.3%) to the references. There was little difference of cox1 gene between sparganum samples of humans and frogs (1-3%). This study is the first report on S. erinaceieuropaei spargana from humans in Liaoning and Hubei Provinces.

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“…Spirometra erinaceieuropaei is a zoonotic parasite causing sparganosis in humans. It has been identified in humans in Vietnam and other countries in Asia, such as Korea, China and Thailand (Anantaphruti et al, 2011;Le et al, 2017;Tang et al, 2017;Kim et al, 2018;Muigg et al, 2019). This larva found in the pig was very small and, thus, could easily have been ignored during meat inspection.…”

Section: Discussionmentioning

confidence: 99%

Occurrence ofTaeniaspecies in pigs in slaughterhouses in Phu Tho province, northern Vietnam

Nguyen¹,

Dermauw²,

Noh

et al. 2020

J. Helminthol.

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Pigs act as the intermediate hosts of the zoonotic tapeworms Taenia solium and Taenia asiatica, as well as of the non-zoonotic Taenia hydatigena. In Vietnam, human taeniasis and cysticercosis have been reported throughout the country; however, data on porcine cysticercosis are scarce. Our study aimed to estimate the prevalence of Taenia spp. in slaughtered pigs in two districts in Phu Tho, a mountainous province in northern Vietnam from where neurocysticercosis patients commonly originate. The carcasses of 399 pigs from 51 small-scale abattoirs were checked for cysticerci, while tongue, liver, masseter muscles, diaphragm and heart were sliced and examined. Retrieved cysticerci underwent polymerase chain reaction–restriction fragment length polymorphism and sequencing for species confirmation. Blood was also collected to detect antibodies by lentil lectin-purified glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP-EITB) and recombinant T24H antigen (rT24H)-EITB and circulating antigens by B158/B60 Ag-ELISA. In two pigs, T. asiatica cysticerci were found, confirming the presence of the parasite in pigs in Vietnam at a low prevalence (0.5%; 95% exact confidence interval (CI): 0–1.19%). Cysticerci of T. solium were found in none of the pigs, although one serum sample was positive for antibodies in both LLGP-EITB and rT24H-EITB. Furthermore, a high prevalence of T. hydatigena cysticercosis was observed (18.0%; 95% Wilson score CI: 14.6–22.1%). In more than half of the T. hydatigena-positive pigs, circulating antigens were detected by Ag-ELISA, confirming that this test cannot be used to diagnose T. solium cysticercosis in this region. Finally, Spirometra erinaceieuropaei was found in one pig liver. It is the first record of this zoonotic cestode species in pigs in Vietnam. Overall, the findings confirmed the complex epidemiology of Taenia spp. in pigs in Vietnam.

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Molecular Identification of Spirometra erinaceieuropaei Tapeworm in Cases of Human Sparganosis, Hong Kong

Cited by 22 publications

References 14 publications

Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis

Development of EST-derived microsatellite markers to investigate the population structure of sparganum — the causative agent of zoonotic sparganosis

Genetic Identification of Spirometra erinaceieuropaei Spargana in Liaoning and Hubei Provinces, PR China

Occurrence ofTaeniaspecies in pigs in slaughterhouses in Phu Tho province, northern Vietnam

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