Molecular Identification ofCeratitis capitata(Diptera: Tephritidae) using DNA Sequences of the COI Barcode Region

Barr, Norman B.; Islam, Md‐Sajedul; Meyer, M. De; McPheron, Bruce A.

doi:10.1603/an11100

Cited by 49 publications

(70 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Barr et al (2012) investigated the utility of DNA barcoding for molecular identification of several tephritid pests, including Ceratitis cosyra . They suggested that the mango fruit fly might include cryptic species.…”

Section: Introductionmentioning

confidence: 99%

Population structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae)

Virgilio¹,

Delatte²,

Nzogela³

et al. 2015

View full text Add to dashboard Cite

The fruit fly Ceratitis cosyra is an important agricultural pest negatively affecting the mango crop production throughout Africa and also feeding on a variety of other wild and cultivated hosts. The occurrence of deeply divergent haplotypes, as well as extensive morphological variability, previously suggested possible cryptic speciation within Ceratitis cosyra. Here we provide the first large-scale characterisation of the population structure of Ceratitis cosyra with the main objective of verifying cryptic genetic variation. A total of 348 specimens from 13 populations were genotyped at 16 polymorphic microsatellite loci. Hardy-Weinberg equilibrium (HWE) deviations were observed in 40.4% of locus-population combinations and suggested the occurrence of genetic substructuring within populations. Discriminant Analysis of Principal Components (DAPC) showed genetic divergence between the vast majority of vouchers from Burundi and Tanzania (plus a few outliers from other African countries) and all other specimens sampled. Individual Bayesian assignments confirmed the existence of two main genotypic groups also occurring in sympatry. These data provided further support to the hypothesis that Ceratitis cosyra might include cryptic species. However, additional integrative taxonomy, possibly combining morphological, ecological and physiological approaches, is required to provide the necessary experimental support to this model.

show abstract

Section: Introductionmentioning

confidence: 99%

Population structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae)

Virgilio¹,

Delatte²,

Nzogela³

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Detection can be accomplished through monitoring of commercial Þelds and surrounding areas for an insect, especially when it can be visually identiÞed (Beers et al 1993). However, when visual identiÞcation is not possible, then genetic methods can be useful for distinguishing benign from harmful taxa (Rugman-Jones et al 2009, Virgilio et al 2009, Barr et al 2012, Liu et al 2011.…”

Section: Discussionmentioning

confidence: 99%

“…three county area, saving the WSDA, USDA, and the local county pest control board approximately US$0.5 million in monitoring, inspection, and control costs. Using genetic methods to detect harmful pest species is not a new approach (Rugman-Jones et al 2009, Virgilio et al 2009, Liu et al 2011, Barr et al 2012). However, our study underscores how integrating genetic methods into a formal Þeld survey can be extremely useful, if not critical, for effective insect management, because rapid identiÞcation can avert costly quarantine.…”

Section: Discussionmentioning

confidence: 99%

Genetic Identification of an Unknown Rhagoletis Fruit Fly (Diptera: Tephritidae) Infesting Chinese Crabapple: Implications for Apple Pest Management

Jean

Egan

Yee³

et al. 2013

jnl. econ. entom.

View full text Add to dashboard Cite

The apple maggot, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), is a serious introduced quarantine pest in the apple (Malus spp.)-growing regions of central Washington and Oregon. In August 2011, seven fly larvae of unknown origin were discovered infesting fruit of an exotic Chinese crabapple, Malus spectabilis (Aiton) Borkhausen, in Kennewick, Benton County, WA. If confirmed, Chinese crabapple would have represented a new host for R. pomonella in Washington and triggered quarantine measures in a surrounding three-county region of the state. Here, we establish, based on five microsatellite loci, the identity of the crabapple-infesting larvae as the western cherry fruit fly, Rhagoletis indifferens Curran, representing a new host record for the fly. Morphological analysis of six flies reared to adulthood confirmed the genetic identification. The results demonstrate the utility of integrating rapid genetic identification methods with field surveys of economic pests, which decreased detection times by months, and avoided enacting costly quarantine measures that saved local and federal bodies > US$0.5 million in monitoring, inspection, and control costs. We discuss current ongoing efforts to develop rapid, accurate, and inexpensive on site DNA-based detection tools for R. pomonella that would have general applicability for the control of pest insects.

show abstract

“…The majority (149 out of 165) of ßies collected in California had DNA extracted using the KingFisher method. An additional 16 samples had DNA extracted from a leg using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA) following the description of Barr et al (2012).…”

Section: Materials Andmentioning

confidence: 99%

“…Sequencing was performed using three BigDye-labeled dideoxynucleotide triphosphates (v3.1 dye terminators, Applied Biosystems, Foster City, CA) and run on an ABI 3730XL DNA analyzer at the Huck InstituteÕs Nucleic Acid Facility (Pennsylvania State University). All sequences were edited and assembled into contigs using the program Sequencher v5 (Genecodes, Ann Arbor, MI), aligned using MEGA5.1 (Tamura et al 2011), and evaluated for pseudogenes as described by Barr et al (2012).…”

Section: Materials Andmentioning

confidence: 99%

Genetic Diversity of Bactrocera dorsalis (Diptera: Tephritidae) on the Hawaiian Islands: Implications for an Introduction Pathway Into California

et al. 2014

View full text Add to dashboard Cite

Population genetic diversity of the oriental fruit fly, Bactrocera dorsalis (Hendel), on the Hawaiian islands of Oahu, Maui, Kauai, and Hawaii (the Big Island) was estimated using DNA sequences of the mitochondrial cytochrome c oxidase subunit I gene. In total, 932 flies representing 36 sampled sites across the four islands were sequenced for a 1,500-bp fragment of the gene named the C1500 marker. Genetic variation was low on the Hawaiian Islands with >96% of flies having just two haplotypes: C1500-Haplotype 1 (63.2%) or C1500-Haplotype 2 (33.3%). The other 33 flies (3.5%) had haplotypes similar to the two dominant haplotypes. No population structure was detected among the islands or within islands. The two haplotypes were present at similar frequencies at each sample site, suggesting that flies on the various islands can be considered one population. Comparison of the Hawaiian data set to DNA sequences of 165 flies from outbreaks in California between 2006 and 2012 indicates that a single-source introduction pathway of Hawaiian origin cannot explain many of the flies in California. Hawaii, however, could not be excluded as a maternal source for 69 flies. There was no clear geographic association for Hawaiian or non-Hawaiian haplotypes in the Bay Area or Los Angeles Basin over time. This suggests that California experienced multiple, independent introductions from different sources.

show abstract

Molecular Identification ofCeratitis capitata(Diptera: Tephritidae) using DNA Sequences of the COI Barcode Region

Cited by 49 publications

References 59 publications

Population structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae)

Population structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae)

Genetic Identification of an Unknown <I>Rhagoletis</I> Fruit Fly (Diptera: Tephritidae) Infesting Chinese Crabapple: Implications for Apple Pest Management

Genetic Diversity of <I>Bactrocera dorsalis</I> (Diptera: Tephritidae) on the Hawaiian Islands: Implications for an Introduction Pathway Into California

Contact Info

Product

Resources

About