Molecular Identification of Fasciola Isolated from the Liver of Meat Animals in Fars Province, Iran

Abstract

Background. Fasciola hepatica and Fasciola gigantica are flatworms that infect animals and humans. Fasciola is the parasite of the liver or bile ducts and intestines of mammals, where such animals are known as their “definite hosts.” The study aims to detect the genotype of Fasciola spp. from the livers of meat animals by using RFLP-PCR in samples collected from Fars province. Methods. Sixty Fasciola spp. samples were collected from infected slaughtered animals in three counties of Fars province, Iran (Jahrom, Nourabad Mamasani, and Kazeroun).Genomic DNA was extracted by the conventional phenol-chloroform method. For the study, PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS1) region from Fasciola species were used to conduct the study. Results. The fragment of about 700 bp in all the Fasciola samples was amplified. In total, 43 samples of Fasciola gigantica and 17 samples of Fasciola hepatica were identified. Conclusion. The dominant Fasciola species in this region is Fasciola gigantica. Hence, it seems that hygienic policies should be developed to prevent and control fascioliasis because of the dominant species, Fasciola gigantica.

Biochemical Liver Functions and Molecular Identification of <i>Fasciola</i> <i>hepatica</i> from Experimentally Infected Rat Model

“…It is worth noting that these genes have been used extensively for characterisation of Fasciola species especially when combined with the restriction fragment length (RFLP) technique [36]. The phylogenetic analysis of Fasciola species was based on a sequence of internal transcribed spacer (ITS1 and ITS2) genes than NADH dehydrogenase I (NDI) gene attributed to the higher substitution rate of mitochondrial DNA than nuclear ribosomal DNA makes the latter suitable for identification and taxonomy of parasites [13]. Therefore, molecular techniques offer some advantages over morphological based identification in terms of accuracy and applicability.…”

“…However, molecular identi-fication makes it possible to characterize the immature stage which would be difficult to differentiate by the morphological features [10]. The genetic characterization and adoption of genome sequencing techniques were employed using the nuclear ibosomal internal transcribed spacer (ITS1 and ITS2) and mitochondrial DNA markers such as NADH dehydrogenase I (NDI) genes [11] [12] [13]. Therefore, the current study was designed to assess the serum biochemical parameters and protein electrophoresis related to liver functions of rats experimentally infected with Fasciola hepatica during the 14 weeks post-infection (WPI).…”

Molecular characterization of Fasciola spp. from ruminants in Duhok province using the ITS1 ribosomal DNA marker

“…According to the findings of the current study, F. hepatica and F. gigantica are the only two Fasciola species isolated from infected ruminants (sheep, goats, and cattle) in the Duhok province as described by other research in the Erbil, like Mohammed et al (47) in Duhok province based on ITS1 and ITS2 markers, Raoof et al (48) in Sulaymaniyah province based on the mitochondrial 28S rRNA gene, and others from different parts of Iraq. Previous studies in neighboring countries stated that both Fasciola species (F. hepatica and F. gigantica) were present in domestic ruminants from different geographical and climatic areas (49,50).…”

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

“…DNA‐based molecular methods have been used to understand the nature and extent of inter‐ and intraspecific variations in Fasciola . Different tools and genetic markers have been used including polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) on the nuclear internal transcribed spacers (ITS1 and/or ITS2) (Anh et al., 2018 ; Mir et al., 2019 ; Rokni et al., 2010 ; Saadatnia et al., 2022 ; Shafiei et al., 2013 ) and mitochondrial cytochrome c oxidase subunit 1 and/or NADH dehydrogenase 1 (Farjallah et al., 2013 ; Itagaki et al., 2005 ). However, using these molecular techniques, it is difficult to reliably differentiate intermediate or hybrid forms of Fasciola .…”