Molecular identification of enteroviruses associated with aseptic meningitis in children from India

Kumar, Arvind; Shukla, Deepti; Kumar, Rashmi; Idris, M Z; Jauhari, Prashant; Srivastava, Shalini; Dhole, Tapan N.

doi:10.1007/s00705-012-1476-7

Cited by 31 publications

(24 citation statements)

References 26 publications

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“…E13, the predominant serotype, has been isolated rarely ( 3 , 10 ); however, nonpolio HEVs have been reported from India in connection with sporadic and epidemic meningitis/encephalitis cases ( 11 , 12 ). A wide spectrum of illnesses have been reported with E13 ( 13 ), so finding E13-associated AFP cases mimicking poliomyelitis ( 14 ) is not surprising.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variants of Echovirus 13, Northern India, 2010

Maan¹,

Chowdhary²,

Shakya³

et al. 2013

Emerg. Infect. Dis.

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Section: Discussionmentioning

confidence: 99%

Genetic Variants of Echovirus 13, Northern India, 2010

Maan¹,

Chowdhary²,

Shakya³

et al. 2013

Emerg. Infect. Dis.

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“…The majority of previous studies have shown that HEVs are responsible for high percentages of all aseptic meningitis cases, ranging from 43 to 83% (Gharbi et al, 2006;Kumar et al, 2013;Papadakis et al, 2013 This copy is for personal use only -distribution prohibited.…”

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confidence: 99%

Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014

Wieczorek

Figas

Krzysztoszek

2016

Polish Journal of Microbiology

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A b s t r a c tA 4-year study (2011)(2012)(2013)(2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were positive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively). The highest frequency of neurological infections (32.7%) occurred in children aged 5-9 years, mostly males (63.9%).K e y w o r d s: aseptic meningitis, cerebrospinal fluid, diagnostic PCR, enteroviruses

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“…Globally, a genotype could be assigned to 58.0% of the specimens, with a clear relationship between the ability to type and the viral load. The overall sensitivity of the methods targeting the four A to D species of human EV on clinical specimens without cell culture amplification is not very high (approximately 50%), even when CODEHOP degenerated primers are used ( (this study). Several studies have reported higher typing performance either with a panel of specimens containing exclusively EV‐B strains or with species B‐specific primers .…”

Section: Discussionmentioning

confidence: 81%

Partial sequencing of the VP2 capsid gene for direct enterovirus genotyping in clinical specimens

Ibrahim

Boukhadra

Nasri-Zoghlami

et al. 2014

Clinical Microbiology and Infection

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Typing of human enterovirus (EV) remains a major goal for diagnostic and epidemiological purposes. Whereas sequencing of the VP1 coding region is the reference standard for EV typing, a method relying on sequencing of the VP2 coding region has been proposed as an alternative; however, this has been validated only on cell culture supernatants. To avoid the selection of cultivable strains and to quicken the identification step, a new semi-nested PCR method targeting the VP2 region was developed by use of the CODEHOP strategy. After validation of the method on reference and clinical strains, a total of 352 clinical specimens found to be positive for EV RNA (138 with the GeneXpert EV kit and 214 with the Enterovirus R-gene kit) during a 3-year period (2010-2012) were analysed prospectively for VP2 genotyping. Overall, 204 (58%) specimens were typeable. A higher proportion of throat swab/stool specimens than of cerebrospinal fluid (CSF) specimens was found to be typeable (94 of 142 (66.2%) vs. 83 of 169 (49.1%), respectively, p <0.01 by the chi-square test). Moreover, the median Ct value obtained was lower for typeable specimens than for untypeable specimens (32.20 vs. 33.01, p <0.05, and 25.96 vs. 31.74, p <0.001, for the GeneXpert and R-gene tests, respectively, by the Mann-Whitney-Wilcoxon test). These results suggest that, in cases of EV meningitis, a peripheral specimen (i.e. throat swab or stool) that is susceptible to exhibiting a higher viral load should be used in preference to CSF for identifying the causative EV genotype by use of the VP2 typing method without cell culture isolation.

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Molecular identification of enteroviruses associated with aseptic meningitis in children from India

Cited by 31 publications

References 26 publications

Genetic Variants of Echovirus 13, Northern India, 2010

Genetic Variants of Echovirus 13, Northern India, 2010

Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014

Partial sequencing of the VP2 capsid gene for direct enterovirus genotyping in clinical specimens

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