Molecular identification of Eimeria hestermani and Eimeria prionotemni from a red-necked wallaby (Macropodidae; Macropus rufogriseus) in Japan

Ekawasti, Fitrine; Kitagawa, Kazuya; Domae, Hiroshi; Wardhana, April Hari; Shibahara, Tomoyuki; Uni, Shigehiko; Tokoro, Masaharu; Sasai, Kazumi; Matsubayashi, Makoto

doi:10.1007/s00436-020-06618-2

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…Eimeria oocysts have been found to be more chemical and enzymatically resistant. Several techniques based on chemical breakdown of the oocyst shell, as well as long-term incubations and freeze-thawing the oocyst, have been created to overcome this limitation [5,18].…”

Section: Nested Duplex Pcr Assaymentioning

confidence: 99%

Development of nested duplex PCR assays for detection of pathogen Eimeria species in cattle in Papua, Indonesia

Ekawasti

Nashrulloh

Nurcahyo

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Eimeria bovis and Eimeria zuernii are highly pathogenic Eimeria species in cattle that are the most prevalent causes of a severe clinical illness characterized by hemorrhagic diarrhea in calves and young cattle with potentially fatal effects over the world. The oocysts of a handful of the known bovine eimeriosis species are difficult to distinguish morphologically. For the specific differentiating evidence of Eimeria species, symptomatic research institutions are increasingly relying on DNA-based technologies. This research offers a duplex polymerase chain reaction (PCR) test based on the internal transcribed spacer-1 (ITS-1) gene that may be used to diagnose E. bovis and E. zuernii in cattle from various locations at the same time. The oocysts were concentrated and purified using a fecal harvesting method. The genomic DNA is extracted according to the instructions included with the kit. Primer pairs specific to each species, as well as a standard optimum annealing temperature of 55°C for these species, were discovered. The samples were amplified in a homogenous way, resulting in a homogeneous band ladder, revealing that the test could distinguish between two highly pathogenic Eimeria species in one tube reaction. This duplex PCR assay can detect a high pathogenic bovine Eimeria simultaneously in a rapid and low cost.

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Section: Nested Duplex Pcr Assaymentioning

confidence: 99%

Development of nested duplex PCR assays for detection of pathogen Eimeria species in cattle in Papua, Indonesia

Ekawasti

Nashrulloh

Nurcahyo

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…(Barker et al, 1989), rock wallaby (Petrogale spp. Gray, 1827) (Barker et al, 1988b;O'Callaghan et al, 1998), red-necked wallaby (Macropus rufogriseus Desmarest, 1817) (Ekawasti et al, 2020) and in the quokka (Setonix brachyurus Quoy and Gaimard, 1830) (Austen et al, 2014;Barker et al, 1988a).…”

Section: Introductionmentioning

confidence: 99%

“…in the western grey kangaroo ( Macropus fuliginosus Desmarest, 1817), eastern grey kangaroo ( Macropus giganteus Shaw, 1790), black‐striped wallaby ( Macropus dorsalis Gray, 1837), tammar wallaby ( Macropus eugenii Desmarest, 1817), euro ( Macropus robustus Gould, 1841), western brush wallaby ( Macropus irma Jourdan, 1837), whip‐tailed wallaby ( Macropus parryi Bennet, 1835), parma wallaby ( Macropus parma Waterhouse, 1845), the agile wallaby ( Macropus agilis Gould, 1842) (Barker et al., 1989 ), rock wallaby ( Petrogale spp. Gray, 1827) (Barker et al., 1988b ; O'Callaghan et al., 1998 ), red‐necked wallaby ( Macropus rufogriseus Desmarest, 1817) (Ekawasti et al., 2020 ) and in the quokka ( Setonix brachyurus Quoy and Gaimard, 1830) (Austen et al., 2014 ; Barker et al., 1988a ).…”

Section: Introductionmentioning

confidence: 99%

Identification and genetic characterization of a novel species of Choleoeimeria Schneider, 1875 from a captive‐bred bilby (Thylacomyidae; Macrotis lagotis) (Reid, 1837) in Western Australia

Brice,

Gao,

Berto

et al. 2024

Ecology and Evolution

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A novel Eimeria sp. from a captive‐bred bilby (Macrotis lagotis Reid, 1837) has been identified in Western Australia. The bilby was bred at the Kanyana Wildlife Rehabilitation Centre, Perth, as part of the National Bilby Recovery Plan. Oocysts (n = 31) irregular blunt ellipsoidal, 17–18 × 11–12 (17.2 × 11.3); length/width (L/W) ratio 1.4–1.5 (1.5). Wall bi‐layered, 0.8–1.0 (0.9) thick, outer layer smooth, c.2/3 of total thickness. Micropyle barely discernible. Oocyst residuum is absent, but 2–3 small polar granules are present. Sporocysts (n = 31) ovoidal, 7–8 × 5–6 (7.8 × 5.7); L/W ratio 1.3–1.4 (1.4). Stieda, sub‐Stieda and para‐Stieda bodies absent or indiscernible; sporocyst residuum present, usually as an irregular body consisting of numerous granules that appear to be membrane‐bound or sometimes diffuse among sporozoites. Sporozoites vermiform with a robust refractile body. Further molecular characterization was conducted on the sporulated oocysts. At the 18S locus, it sat in a large clade of the phylogenetic tree with two isolates of Eimeria angustus from quendas (Isoodon obesulus Shaw, 1797) and the Choleoeimeria spp. It shared the highest identity with E. angustus (KU248093) at 98.84%; at the COI gene locus, it was unique and most closely related to Choleoeimeria taggarti, which is hosted by another species of marsupial, the yellow‐footed antechinus (Antechinus flavipes flavipes), with 90.58% genetic similarity. Based on morphological and molecular data, this isolate is a new species and named as Choleoeimeria yangi n. sp.

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“…Twenty-eight single oocysts of the purified Isospora sp. isolate were obtained using disposable glass capillary micropipettes and processed as templates for polymerase chain reaction (PCR) based on previously reported methods [4]. Molecular identification of Isospora sp.…”

mentioning

confidence: 99%

“…was performed by PCR and sequencing using previously reported primer pairs targeting the 18S ribosomal RNA (rRNA) gene [6]. Phylogenetic trees were constructed as described previously [4]. Briefly, sequences were aligned using Clustal X (Version 2.0) [5], and all gaps were deleted.…”

mentioning

confidence: 99%

Phylogenetic characterization of <i>Isospora jaracimrmani</i> oocysts from a veiled chameleon (family Chamaeleonidae; <i>Chamaeleo calyptratus</i>) reared at a zoo in Ishikawa, Japan

Ekawasti

Kitagawa

Domae

et al. 2021

The Journal of Veterinary Medical Science

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Oocysts of Isospora sp. were detected in the feces of a veiled chameleon (family Chamaeleonidae; Chamaeleo calyptratus) kept at a zoo in Ishikawa, Japan. Phylogenetic analysis placed the sequence in the cluster of Isospora spp. isolated from reptiles. Based on a comparison of morphological data of ten previously reported Isospora species from the Chamaeleonidae family, this isolate was morphologically similar to I. jaracimrmani, which has been considered to be a virulent species. This case study suggests the possibility that species of Isospora might not always cause disease because the animal that shed these oocysts showed no symptoms for more than two months.

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Molecular identification of Eimeria hestermani and Eimeria prionotemni from a red-necked wallaby (Macropodidae; Macropus rufogriseus) in Japan

Cited by 7 publications

References 37 publications

Development of nested duplex PCR assays for detection of pathogen Eimeria species in cattle in Papua, Indonesia

Development of nested duplex PCR assays for detection of pathogen Eimeria species in cattle in Papua, Indonesia

Identification and genetic characterization of a novel species of Choleoeimeria Schneider, 1875 from a captive‐bred bilby (Thylacomyidae; Macrotis lagotis) (Reid, 1837) in Western Australia

Phylogenetic characterization of <i>Isospora jaracimrmani</i> oocysts from a veiled chameleon (family Chamaeleonidae; <i>Chamaeleo calyptratus</i>) reared at a zoo in Ishikawa, Japan

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