Molecular identification of disk abalone ( Haliotis discus discus ) tetraspanin 33 and CD63: Insights into potent players in the disk abalone host defense system

Priyathilaka, Thanthrige Thiunuwan; Bathige, S.D.N.K.; Herath, H.M.L.P.B.; Lee, Sukkyoung; Lee, Jehee

doi:10.1016/j.fsi.2017.08.020

Cited by 10 publications

(11 citation statements)

References 61 publications

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“…The predicated amino acid sequence of Cg CD63H consists of four typical TM domains, a short extracellular loop, and a large extracellular loop. Additionally, a CCG motif and several Cys residues were identified in the large extracellular loop of Cg CD63H, which were highly conserved structural features of the previously reported CD63s ( 8 , 22 ). Coincidentally, Cg CD63H was bunched together with CD63 homologs from invertebrates and vertebrates in an unrooted phylogenetic tree.…”

Section: Discussionmentioning

confidence: 60%

“…Several mammalian tetraspanins including CD63 are reported to participate in the recognition of PAMPs through the binding activity with PRRs and tetraspanins traffic at the cell surface ( 11 , 56 ). In invertebrates, CD63 is also suspected to function in recognition process with evidences of the upregulation of mRNA expression after PAMP stimulation ( 22 ). Here, Cg CD63H was found to bind GLU, PGN, and LPS only in the presence of lyophilized hemolymph, indicating that Cg CD63H could combine PAMPs in an indirect manner, possibly with the assistance of other possible PRRs.…”

Section: Discussionmentioning

confidence: 99%

“…The characterization of CD63 has been recently found in invertebrates, for instance, disk abalone Haliotis discus discus ( 22 ) and clam Paphia undulate ( 23 ). Earlier work has observed an interesting phenomenon: under the stimulation with bacteria, virus, and pathogen-associated molecular patterns (PAMPs), the mRNA expressions of these CD63s change significantly.…”

Section: Introductionmentioning

confidence: 99%

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A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas

et al. 2020

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Cluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oyster Crassostrea gigas is, however, yet to be discovered. This work makes contributions by identifying Cg CD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence of Cg CD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly ( p < 0.01) upregulated after the injection of Vibrio splendidus . Cg CD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to the Yarrowia lipolytica -containing phagosomes after the stimulation of Y. lipolytica . The recombinant Cg CD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes toward V. splendidus and Y. lipolytica is significantly inhibited ( p < 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests that Cg CD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing in C. gigas .

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas

et al. 2020

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“…On the other hand, the number of studies assessing the implication of leukocyte-specific tetraspanins in migration and adhesion is limited [57,58]. TSPAN33 is an almost specific leukocyte tetraspanin, as shown by previous studies performed by our and other independent groups [35][36][37]40,59]. It is the last described member in the superfamily and forms part of the evolutionarily conserved TspanC8 subgroup, capable of directly associating with the membrane-anchored metalloprotease ADAM10, regulating its trafficking and function [60,61].…”

Section: Discussionmentioning

confidence: 99%

Tetraspanin 33 (TSPAN33) regulates endocytosis and migration of human B lymphocytes by affecting the tension of the plasma membrane

et al. 2020

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“…Interestingly, the existence of tetraspanins in the innate immune system of invertebrates and non-mammalian vertebrates has also been described. Marine gastropod mollusks show ubiquitous expression of CD63 and Tspn33, which are upregulated upon different immune stimulation challenges, like toll-like receptor (TLR) ligands, bacteria or viral infection ( 23 ). Similarly, CD9 expression is induced in lamprey fish after LPS stimulation ( 24 ), or in turtles after bacterial infection ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Tetraspanins as Organizers of Antigen-Presenting Cell Function

2018

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Professional antigen-presenting cells (APCs) include dendritic cells, monocytes, and B cells. APCs internalize and process antigens, producing immunogenic peptides that enable antigen presentation to T lymphocytes, which provide the signals that trigger T-cell activation, proliferation, and differentiation, and lead to adaptive immune responses. After detection of microbial antigens through pattern recognition receptors (PRRs), APCs migrate to secondary lymphoid organs where antigen presentation to T lymphocytes takes place. Tetraspanins are membrane proteins that organize specialized membrane platforms, called tetraspanin-enriched microdomains, which integrate membrane receptors, like PRR and major histocompatibility complex class II (MHC-II), adhesion proteins, and signaling molecules. Importantly, through the modulation of the function of their associated membrane partners, tetraspanins regulate different steps of the immune response. Several tetraspanins can positively or negatively regulate the activation threshold of immune receptors. They also play a role during migration of APCs by controlling the surface levels and spatial arrangement of adhesion molecules and their subsequent intracellular signaling. Finally, tetraspanins participate in antigen processing and are important for priming of naïve T cells through the control of T-cell co-stimulation and MHC-II-dependent antigen presentation. In this review, we discuss the role of tetraspanins in APC biology and their involvement in effective immune responses.

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Molecular identification of disk abalone ( Haliotis discus discus ) tetraspanin 33 and CD63: Insights into potent players in the disk abalone host defense system

Cited by 10 publications

References 61 publications

A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas

A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of Oyster Crassostrea gigas

Tetraspanin 33 (TSPAN33) regulates endocytosis and migration of human B lymphocytes by affecting the tension of the plasma membrane

Tetraspanins as Organizers of Antigen-Presenting Cell Function

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