Molecular identification of decay fungi in the wood of urban trees

Abstract

Trees are valuable for urban areas, however, are also susceptible to wood rot fungi. For accurate and fast assessment of the severity and evolution of decay in standing trees, a molecular technique was used to identify the causal agents of wood rot. Fruit bodies of wood decay fungi were collected from infected trees in various stands in Germany. Thirty-six species were identified by traditional methods. The DNA of fruit bodies was extracted, ITS-rDNA amplified by PCR, and ITS regions sequenced. Wood samples from infected urban trees were collected, the entire DNA extracted from affected wood parts, and fungal ITS amplified and sequenced. Fungal species were identified by comparing sequence data with the fruit body data. The technique enables an accurate and rapid identification of causal rot fungi in urban trees.

Influence of xylem ray integrity and degree of polymerization on bending strength of beech wood decayed by Pleurotus ostreatus and Trametes versicolor

“…The sources of fungi are described in previous publications (Bari et al, 2015c). Fungi had been verified in accordance with Schmidt et al (2012).…”

In vivo investigation of chemical alteration in oak wood decayed by Pleurotus ostreatus

“…from the Guilan forest (Iran). Morphological identification was performed according to Ryvarden andGilbertson (1993, 1994) and molecular identification was performed in according to Schmidt et al (2012) and Bari (2014). In brief, DNA of the infected wood and the fungal fruiting body was extracted, PCR was performed via ITS1 and ITS4 primers, electrophoresed, purified, and sequenced.…”

Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood

“…at Alamdardeh forest (located in Sari, Iran). For molecular verification of the fungal species, pure cultures obtained from the fruiting bodies of the fungi were identified by rDNA-ITS analysis according to Schmidt et al (2012). Fungal DNA was extracted, amplified by polymerase chain reaction (PCR) of the ITS region and sequenced.…”