Molecular identification of Corynebacterium pseudotuberculosis in sheep

Abstract

Caseous lymphadenitis is still a serious zoonotic problem in Turkey. Sheep suffer from the disease with yield loss in wool and meat production. Moreover, with inexperienced laboratory staff, biochemical identification may go unrevealed. The scope of this study was to demonstrate the presence of Corynebacterium pseudotuberculosis in sheep by PCR. The sampling was conducted via collecting lymph fluids from the lymph node internal pouch wall of 100 sheep that were examined for the presence of Corynebacterium pseudotuberculosis. Molecular identification of the Corynebacterium pseudotuberculosis isolates was carried out by establishing the presence of the proline iminopeptidase gene. All isolates were confirmed to be Corynebacterium pseudotuberculosis by polymerase chain reaction. The polymerase chain reaction procedure conducted in this research was observed to be reliable and fast, and could be utilized for confirmation of caseous lymphadenitis in sheep as an optional technique to timeconsuming biochemical identification methods. Pleomorphic bacteria, caseous lymphadenitis, proline iminopeptidase, PCR

Comparative Diagnosis of Caseous Lymphadenitis in Sheep by Molecular and ELISA Method

“…(%16) ve Pasteurella spp. (%13) izole edildiği bildirilmiştir (33). Bu çalışma sonucunda bakteriyolojik kültürde C. pseudotuberculosis (%13.3) izolasyonuna ek olarak S. aureus, (%17.7), Streptococcus spp.…”

Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors

“…Caseous lymphadenitis causes thick encapsulated abscesses in the lymph nodes and internal organs (especially in the lungs and mediastinal lymph nodes), containing creamy, yellow-green colored pus (Tolu and Savas, 2014;Akgül et al, 2018). The form of the disease that causes abscesses in the superficial lymph nodes and subcutaneous tissues is called external or superficial caseous lymphadenitis, and the form that causes abscesses in the internal organs is called internal or visceral caseous lymphadenitis (Ilhan, 2008;Ipek et al, 2012;Kumar et al, 2012;Basbug et al, 2014;Tolu and Savas, 2014;Oreiby, 2015;Akgül et al, 2018;Parin et al, 2018;Ilhan, 2020). Visceral caseous lymphadenitis is more likely to be seen in sheep than in goats.…”

“…Caseous lymphadenitis cases may cause abortions in pregnant sheep. It rarely causes death, especially in young animals (Oreiby, 2015;Parin et al, 2018;Ilhan, 2020). Superficial caseous lymphadenitis is transmitted to healthy animals as a result of physical contact with agents scattered around as a result of rupture of abscess foci (Fontaine and Baird, 2008).…”