Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan

Kong, Qingming; Fan, Lanfen; Jun-he, Zhang; Akao, Nobuaki; Dong, Kewei; Lou, Di; Ding, Jianzu; Tong, Qunbo; Zheng, Bin; Chen, Rui; Ohta, Nobuhiro; Lu, Shaohong

doi:10.1016/j.ijfoodmicro.2015.01.007

Cited by 35 publications

(34 citation statements)

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“…The high prevalence of Anisakis species in fish hosts in this study is therefore thought to reflect the richness of micro/macroinvertebrates and possibly subsequently have relations with the biomass of other predators such as pinnipeds and cetaceans in waters investigated. Several papers also reported relatively a high prevalence of anisakid nematodes larvae in the largehead hairtail (Borges et al 2012;Kong et al 2015;Umehara et al 2010), as in this study. The low number of the nematodes larvae in the previous study (Lee et al 2009) may just reflect the low abundance of micro/macroinvertebrates in the areas where the largehead hairtail were caught.…”

Section: Discussionsupporting

confidence: 82%

“…Several papers described anisakid nematodes L3 from the largehead hairtail (Borges et al 2012;Kong et al 2015;Lee et al 2009;Shih 2004;Umehara et al 2010;Zhang et al, 2013), but there was no report in which T. japonicus was mentioned as host species, except for our study. It is not clear if the host species mentioned was in fact T. japonicus or other Trichiurus species in their studies because the identification of host species was not clearly conducted.…”

Section: Discussionmentioning

confidence: 55%

“…There have been many papers regarding anisakid nematodes isolated from Trichiurus species (Borges et al 2012;Kong et al 2015;Lee et al 2009;Shih 2004;Umehara et al 2010;Zhang et al 2013). But, the identification of host species was not clearly conducted, and several scientific names (e.g., Trichiurus lepturus, Trichiurus haumela, Trichiurus sp.)…”

Section: Introductionmentioning

confidence: 99%

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Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

2016

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Background: The nematode species belonging to genus Anisakis occur at their third larval stage in numerous marine teleost fish species worldwide and known to cause accidental human infection through the ingestion of raw or undercooked fish or squids. They may also draw the attention of consumers because of the visual impact of both alive and dead worms. Therefore, the information on their geographical distribution and clear species identification is important for epidemiological survey and further prevention of human infection. Results: For identification of anisakid nematodes species isolated from largehead hairtail (Trichiurus japonicus), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers of ribosomal DNA were conducted. Mitochondrial cytochrome c oxidase subunit 2 gene was also sequenced, and phylogenetic analysis was conducted. From the largehead hairtail (n = 9), 1259 nematodes were isolated in total. Most of the nematodes were found encapsulated throughout the viscera (56.2 %, 708/1259) or moving freely in the body cavity (41.5 %, 523/1259), and only 0.3 % (4/1259) was found in the muscles. By PCR-RFLP, three different nematode species were identified. Anisakis pegreffii was the most dominantly found (98. 7 %, 1243/1259) from the largehead hairtail, occupying 98.7 % (699/708) of the nematodes in the mesenteries and 98.1 % (513/523) in the body cavity. Hybrid genotype (Anisakis simplex × A. pegreffii) occupied 0.5 %, and Hysterothylacium sp. occupied 0.2 % of the nematodes isolated in this study. Conclusions:The largehead hairtail may not significantly contribute accidental human infection of anisakid nematode third stage larvae because most of the nematodes were found from the viscera or body cavity, which are not consumed raw. But, a high prevalence of anisakid nematode larvae in the largehead hairtail is still in concern because they may raise food safety problems to consumers. Immediate evisceration or freezing of fish after catch will be necessary before consumption.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

2016

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“…In Japan, S. japonicus was infected by A. simplex and A. pegreffii larvae, together with a few larvae of other anisakid species (Arizono et al, 2012). Anisakis typica, recombinant genotype of A. simplex s. s. and A. pegreffii, H. amoyense and H. fabri were identified in East China Sea (Kong et al, 2015). The domains SJ1 and SJ2 of the larvae in S. japonicus were 70% homologous to that of A. simplex (Table 3).…”

Section: Its Domain Analysismentioning

confidence: 99%

“…(Quiazon et al, 2009;Sohn et al, 2014). High prevalence of Anisakidae larvae infection was reported in fish from in the East China Sea (Kong et al, 2015). The specific identification of Anisakid larvae L3 was complex, because few morphological characteristics could be used for identification in the larval stage.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Anisakis Larvae in Commercial Fish along the Northern Coast of Taiwan

Yang¹,

Chen²,

Wu³

2015

Research J. of Parasitology

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Anisakis larvae of three commercial fish species, including Trichiurus lepturus, Scomber japonicus and Trachurus japonicus, found in the ocean North of Taiwan, were examined. The morphology of the larvae was analyzed using light and scanning electron microscopes, the population dynamics (including prevalence, mean intensity and abundance) were studied and the molecular characteristics of the Anisakid nematodes were identified. The annual prevalence of the larvae in T. lepturus, S. japonicus and T. japonicus were 91, 39 and 89%, respectively. Using light microscopy and scanning electron microscopy demonstrated that the worms in the three species of fish showed no difference in morphological features. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) indicated that the larvae from the three species of fish belonged to a single species. However, using phylogenetic analysis in the molecular identification of ribosomal DNA (rDNA) domains ITS-1 (internal transcribed spacer 1) and ITS-2, the larvae in the three species of fish were not of a single species of Anisakis.

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Hysterothylacium amoyense in Platycephalous indicus: a Persian Gulf fish and its experimental infection of mouse model

et al. 2016

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Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan

Cited by 35 publications

References 50 publications

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

Occurrence of Anisakis Larvae in Commercial Fish along the Northern Coast of Taiwan

Hysterothylacium amoyense in Platycephalous indicus: a Persian Gulf fish and its experimental infection of mouse model

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