Molecular identification of a new androgenic gland-specific insulin-like gene from the mud crab, Scylla paramamosain

Zhang, Yaqun; Qiao, Kun; Wang, Shuping; Peng, Hui; Shan, Zhongguo; Wang, Kejian

doi:10.1016/j.aquaculture.2014.06.033

Cited by 30 publications

(21 citation statements)

References 38 publications

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“…The effects of eyestalk ablation are well characterized in male Decapoda causing the hypertrophy and hyperplasia of AG cells [Khalaila et al, 2002] and leading to an increase in IAG expression [Rosen et al, 2010], which ultimately results in accelerated testicular development [Nagaraju, 2011;Zhang et al, 2014]. This effect was indeed noted in S. verreauxi , seen through the comparison of AG1 and AG2, although this difference must be considered in the context of individual variation.…”

Section: Functional Characterization Of the Mature Androgenic Gland Dmentioning

confidence: 91%

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster, Sagmariasus verreauxi

Chandler

Aizen²,

Elizur³

et al. 2015

Sex Dev

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The Eastern spiny lobster, Sagmariasus verreauxi, is commercially important in fisheries, with growing aquaculture potential, driving an interest to better understand male sexual differentiation. Amongst the Decapoda, the androgenic gland (AG) and the insulin-like androgenic gland hormone (IAG) have a well-defined function in male sexual differentiation. However, IAG is not a sex determinant and therefore must be considered as part of a broader, integrated pathway. This work uses a transcriptomic, multi-tissue approach to provide an integrated description of male-biased expression as mediated through the AG. Transcriptomic analyses demonstrate that IAG expression is stage- and eyestalk-regulated (low in immature, high in mature and 6-times higher in hypertrophied glands), with IAG being the predominant AG-specific factor. The low expression of this key factor in immature males suggests the involvement of other tissues in male sexual differentiation. Across tissues, the gonad (87.8%) and antennal gland (73.5%) show the highest male-biased differential expression of transcripts and also express 4 sex-determination regulators, known as Dmrts, with broader expression of Sv-Sxl and Sv-TRA-2. In order to better understand male sexual differentiation, tissues other than the AG must also be considered. This research highlights the gonad and antennal gland as showing significant male-biased expression patterns, including the Sv-Dmrts.

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Section: Functional Characterization Of the Mature Androgenic Gland Dmentioning

confidence: 91%

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster, Sagmariasus verreauxi

Chandler

Aizen²,

Elizur³

et al. 2015

Sex Dev

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“…For example, in the spot shrimp Pandalus platyceros and the swimming crab Portunus pelagicus , the hypertrophic AG and the increased numbers of IAG-producing cells were observed in males that had bilateral eyestalks removed ( Hoffman, 1968 ; Sroyraya et al, 2010 ). Likewise, in the mud crab Scylla paramamosain and the blue crab C. sapidus , eyestalk ablation resulted in the up-regulation of IAG expression in AG ( Chung et al, 2011 ; Zhang et al, 2014 ). These previous studies all demonstrated that the activity of AG was inhibited by the specific substance(s) derived from the eyestalk; however, the exact mechanism(s) responsible for this inhibition is still unknown ( Khalaila et al, 2002 ).…”

Section: Introductionmentioning

confidence: 99%

Crustacean Female Sex Hormone From the Mud Crab Scylla paramamosain Is Highly Expressed in Prepubertal Males and Inhibits the Development of Androgenic Gland

Liu

et al. 2018

Front. Physiol.

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Recently, the crustacean female sex hormone (CFSH), which is considered a female-specific hormone, has been shown to play a crucial role in female phenotypes in crustaceans. In this study, two transcripts (Sp-CFSH1 and Sp-CFSH2) encoding the same CFSH precursor were cloned from the mud crab Scylla paramamosain. Homology and phylogenetic analysis showed that CFSHs were homologous to interleukin-17 and highly conserved among brachyuran crabs. PCR analysis revealed that Sp-CFSH was expressed exclusively in the eyestalk ganglion of both prepubertal males and females, and surprisingly, the abundance of Sp-CFSH transcripts detected in the males were not significantly different from that of the females (P > 0.05). In addition, mRNA in situ hybridization showed that Sp-CFSH was localized in the X-organ of the male eyestalk ganglion. During the development of the androgenic gland (AG), the level of Sp-IAG mRNA in AG remained at low levels from stages I to II (early stage) but had a significant increase at stage III (mature stage). In contrast, the level of Sp-CFSH transcripts in the eyestalk ganglion was high in the early stage but extremely low in the mature stage. To investigate the potential function of CFSH in male S. paramamosain, the recombinant protein (∼20 kDa) was expressed in Escherichia coli and was subsequently added to AG explants in vitro. It was demonstrated that recombinant Sp-CFSH protein significantly reduced the expression of Sp-IAG in the AG explants at a concentration of 10−6 M (P < 0.05). In conclusion, our study provides the first piece of evidence that shows CFSH from the eyestalk ganglion acts as a negative regulator inhibiting the development of AG in crustaceans.

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“…This pattern is not reported in other taxa of decapods characterized by heterochely (Wilson, 1902(Wilson, -1903Cheung, 1976;Hamilton et al, 1976;Simonson, 1985;Govind et al, 1988;Haefner, 1990;Yamaguchi, 1995). Based in Yamaguchi (1977), the specimen collected in the present study has probably not gone through this stage and both chelipeds have undergone hypertrophy, or changes possibly occurred in the expression of genes such as the Hox genes, responsible for the expression of body architecture, including the development of thoracic appendices (Averof & Patel, 1997;Kaufman & Abzhanov, 2000) or Sp-IAG genes, responsible for the androgenic gland hormone expression that operates in the development of sexual characteristics (Zhang et al, 2014).…”

Section: R E S U L T Smentioning

confidence: 85%

First record of bilateral hypertrophy in chelas of Uca rapax male specimen (Crustacea, Decapoda, Ocypodidae) on the Brazilian coastline

Vale

Alencar

Moraes

et al. 2015

Mar. Biodivers. Rec.

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An adult male specimen ofUca rapax(Crustacea, Decapoda, Ocypodidae), containing bilaterally hypertrophied chelipeds, was found in the mangrove area of the Rio Grande do Norte state, north-eastern Brazil. The chelipeds are subequal in size and similar to the major cheliped of normal male specimens ofUca rapax. This paper is the first record of the aforementioned anomaly for the species from the Brazilian coastline.

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Molecular identification of a new androgenic gland-specific insulin-like gene from the mud crab, Scylla paramamosain

Cited by 30 publications

References 38 publications

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b>

Crustacean Female Sex Hormone From the Mud Crab Scylla paramamosain Is Highly Expressed in Prepubertal Males and Inhibits the Development of Androgenic Gland

First record of bilateral hypertrophy in chelas of Uca rapax male specimen (Crustacea, Decapoda, Ocypodidae) on the Brazilian coastline

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