Molecular identification, morphological characterization and new insights into the ecology of larval Pseudoterranova cattani in fishes from the Argentine coast with its differentiation from the Antarctic species, P. decipiens sp. E (Nematoda: Anisakidae)

Timi, Juan T.; Paoletti, Michela; Cimmaruta, Roberta; Lanfranchi, Ana L.; Alarcos, Ana Julia; Garbin, Lucas; George-Nascimento, Mario; Rodríguez, Diego H.; Giardino, Gisela V.; Mattiucci, Simonetta

doi:10.1016/j.vetpar.2013.09.033

Cited by 48 publications

(56 citation statements)

References 77 publications

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“…In the IB tree (Figure 4), the same group of sequences exhibited a reasonable posterior probability (58%) and a higher bootstrap value (89%). In accordance with Timi et al (2014), our phylogenetic trees strongly support P. decipiens sp. E (accession number HM 147282-1) as a sister taxon to the remaining species of the P. decipiens complex.…”

Section: Resultssupporting

confidence: 86%

“…Members of the Anisakidae Skrjabin & Karokhin, 1945 in cod include species of the genus Pseudoterranova Mozgovoi, 1950, whose larvae reportedly cause human anisakiasis and pseudoterranoviasis through the ingestion of inadequately cooked fish (MATTIUCCI & NASCETTI, 2008;PERDIGUERO-ALONSO et al, 2008;TIMI et al, 2014). Although this genus has been known mainly in terms of the species complex Pseudoterranova decipiens (sensu lato), it actually includes the following species: Pseudoterranova decipiens Krabbe, 1878 (sensu stricto); P. krabbei Paggi, Mattiucci, Gibson, Berland, Nascetti, Cianchi & Bullini, 2000;P.…”

Section: Introductionmentioning

confidence: 99%

“…cattani George-Nascimento & Urrutia, 2000;P. kogiae (Johnston & Mawson, 1939); and P. ceticola (Deardorff & Overstreet, 1981) (see MCCLELLAND, 2002;MATTIUCCI & NASCETTI, 2008;ARIZONO et al, 2011;TIMI et al, 2014). These larvae are difficult to differentiate and identify to the level of species based solely on morphology because there are few or no diagnostic characters at this level; hence, the need for species assignation based on genetic analyses.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil

Mafra

Mantovani

Borges³

et al. 2015

Rev. Bras. Parasitol. Vet.

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An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed.Keywords: Anisakiasis, codworm, food safety, public health. ResumoUma larva de Nematoda anisakídeo encontrada em bacalhau comercializado no Estado de Minas Gerais, Brasil foi estudada por microscopias de luz e eletrônica de varredura e por uma abordagem molecular. As regiões da subunidade 2 da citocromo c-oxidase mitocondrial (mtDNA cox-2), 28S rRNA e ITS1, 5.8S e ITS2 foram amplificadas usando a reação em cadeia da polimerase e sequenciadas para avaliar as relações filogenéticas da larva. O perfil genético confirmou que esta larva pertence à espécie Pseudoterranova decipiens (sensu stricto). Esse é o primeiro estudo molecular e ultraestrutural de Pseudoterranova decipiens (sensu stricto) de bacalhau importado vendido no Brasil. As implicações destes resultados para a saúde são discutidas.Palavras-chave: Anisakíase, bacalhau, segurança alimentar, saúde pública.

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil

Mafra

Mantovani

Borges³

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…P. decipiens s. l., as reported by Szostakowska et al, [35], occurs sporadically and in our work only in 2 fish species were found (C. conger and Z. faber from FAO area 27) confirming that only parasites belonging to the P. decipiens complex are present in the NE Atlantic Ocean. P. cattani was found in G. blacoides from FAO area 41, in agreement with Timi et al [36]. H. aduncum and C. osculatum s.l.…”

Section: Parasites Distributionsupporting

confidence: 89%

Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas

et al. 2020

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Background: The presence of Anisakis larvae in fish represents a major public health concern. Effective risk management procedures should be applied to prevent heavily infected products from reaching the market. The aim of the study is to provide preliminary data on parasite exposure and risk classification in frozen fish products by applying a risk categorization scheme (site, abundance, density and epidemiology -SADE) and Fish Parasite Rating (FPR) method. Fish and cephalopods samples (N = 771) from 5 different FAO Atlantic areas were examined and categorized after an accurate visual inspection and a chloro-peptic digestion.Results: In 25 out of 33 fish species parasite larvae were found. 10897 anisakids larvae were collected and identified to genus level. Molva dypterygia, Conger conger, Zeus faber and Aphanopus carbo were shown to be the most highly infected species. SADE and FPR scores were 1 and poor, respectively, for the referred species, because of the disseminated Anisakis infection and commercial rejection. Conclusion: SADE/FPR method showed high specificity and accuracy. The information provided in this work could be used in early warning systems for the detection of parasites in fishery products and might help fishing industries in establishing management strategies for infected stocks in terms of cost saving decisions.

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“…Some of these cryptic species show considerable differences in host and geographical ranges789. The biodiversity of ascaridoids is substantial, consistent with the broad range of hosts infected by these nematodes, including birds, mammals, reptiles, fish and amphibians10; there are also differences in ecology of particular host-parasite affiliations, and considerable genetic variation within species11.…”

mentioning

confidence: 90%

Mitochondrial Phylogenomics yields Strongly Supported Hypotheses for Ascaridomorph Nematodes

Nadler

Liu

et al. 2016

Sci Rep

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Ascaridomorph nematodes threaten the health of humans and other animals worldwide. Despite their medical, veterinary and economic importance, the identification of species lineages and establishing their phylogenetic relationships have proved difficult in some cases. Many working hypotheses regarding the phylogeny of ascaridomorphs have been based on single-locus data, most typically nuclear ribosomal RNA. Such single-locus hypotheses lack independent corroboration, and for nuclear rRNA typically lack resolution for deep relationships. As an alternative approach, we analyzed the mitochondrial (mt) genomes of anisakids (~14 kb) from different fish hosts in multiple countries, in combination with those of other ascaridomorphs available in the GenBank database. The circular mt genomes range from 13,948-14,019 bp in size and encode 12 protein-coding genes, 2 ribosomal RNAs and 22 transfer RNA genes. Our analysis showed that the Pseudoterranova decipiens complex consists of at least six cryptic species. In contrast, the hypothesis that Contracaecum ogmorhini represents a complex of cryptic species is not supported by mt genome data. Our analysis recovered several fundamental and uncontroversial ascaridomorph clades, including the monophyly of superfamilies and families, except for Ascaridiidae, which was consistent with the results based on nuclear rRNA analysis. In conclusion, mt genome analysis provided new insights into the phylogeny and taxonomy of ascaridomorph nematodes.

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Molecular identification, morphological characterization and new insights into the ecology of larval Pseudoterranova cattani in fishes from the Argentine coast with its differentiation from the Antarctic species, P. decipiens sp. E (Nematoda: Anisakidae)

Cited by 48 publications

References 77 publications

Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil

Morphological and molecular diagnosis of Pseudoterranova decipiens (sensu stricto) (Anisakidae) in imported cod sold in Brazil

Risk-based scoring and genetic identification for anisakids in frozen fish products from Atlantic FAO areas

Mitochondrial Phylogenomics yields Strongly Supported Hypotheses for Ascaridomorph Nematodes

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