Molecular Identification and Susceptibility Pattern of Clinical <i>Nocardia</i> Species: Emergence of <i>Nocardia crassostreae</i> as an Agent of Invasive Nocardiosis

Taj-Aldeen, Saad J.; Deshmukh, Anand; Doiphode, Sanjay; Wahab, Atqah Abdul; Allangawi, Mona; AlMuzrkchi, A.; Klaassen, Corné H. W.; Meis, Jacques F.

doi:10.1155/2013/256025

Cited by 13 publications

(10 citation statements)

References 49 publications

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“…However, other environmental factors such as reduced water circulation of superficial embayments, warmest temperatures and great nutrient levels can also increase the prevalence of infection of nocardiosis ( Bower et al, 2005 ) as also reported in the PN station as showed by Chl-a values. Interestingly, recent reports assessed for the first time infection of N. crassostreae as emerging human pathogen causing invasive pulmonary nocardiosis in immunosuppressed and immunocompetent patients ( Taj-Aldeen et al, 2013 ; Igbaseimokumo et al, 2016 ). This data suggest N. crassostreae to be a possible emerging disease with consequent possible risk of zoonotic infections.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

et al. 2018

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The bivalve Mytilus galloprovincialis has a broad geographic distribution, represent an important species for the ecology of coastal waters, also constituting a major aquaculture species. In the present work, molecular and tissue biomarkers were examined in mussel populations (M. galloprovincialis) located in four different areas of the coastal water of the Campania Region. During an annual life cycle, we analyzed the expression patterns of several genes commonly used to estimate cellular stress response and damage, namely p53, p63, HSP70, MT-10, and MT-20, related tissue lesions (pathogens, inflammations, digestive tubules damage), oxidative stress indicators (H2O2, SOD specific activity) and associated environmental data. The computed Principal Component Analysis showed that the areas were discernible based on the environmental data and biomarker results. About animal health status, mussels from Gulf of Pozzuoli and Naples’s harbor did show a thinnest epithelial cell of digestive tubules compared to mussels sampled from other sampling sites; moreover, high prevalence of cases of intersex in three of the examinated areas were observed. The presence of a potential zoonotic pathogen (Nocardia crassostreae) was identified, appearing as an important possible emerging disease. We also reported the OIE notifiable protozoa Marteilia refringens in three areas out of four. The likely impact of both observed pathogens on the mussel health and shellfish aquaculture needs to be urgently addressed. Results are discussed considering animal histopathological health parameters and biological effects.

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Section: Discussionmentioning

confidence: 99%

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

et al. 2018

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“…Therefore, molecular methods with sufficient specificity and sensitivity should be employed. Morphological, physiological, and molecular characteristics can play a crucial role to identify Nocardia species (20). While no valuable standard therapy is available for nocardiosis, surgical excision is successfully applied in a number of cases.…”

Section: Discussionmentioning

confidence: 99%

Disseminated Nocardiosis in an Immunocompetent Individual Infected with Nocardia brasiliensis, an Opportunistic Agent

Mardani

Aslani

Geramishoar

et al. 2016

Arch Clin Infect Dis

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Introduction: Nocardia species are described as opportunistic pathogens that mainly cause pulmonary nocardiosis in immunocompromised individuals, particularly in patients with acquired immune deficiency syndrome (AIDS), intravenous drug abusers, strongly burned victims, the Cushing syndrome, the ones underlying transplantation, and prolonged use of corticosteroids. Case Presentation: The current paper reported a case of disseminated nocardiosis in a 28-year-old Iranian male who had type 2 diabetes mellitus with prolonged use of corticosteroid for the last 5 years. He was admitted to the department of infectious diseases at Shahid Beheshti hospital, Tehran, Iran, following a complaint of pneumonia (chest pain) and presented a subcutaneous purple nodule on the left thigh without pain. Direct microscopic examination revealed numerous filamentous branching and rod-shaped bacilli. The tentative diagnosis of chronic inflammation with nocardiosis was made. In addition, tiny, chalky white and irregular colonies emitting an earthy odor appeared on blood and chocolate agar and were identified as Nocardia brasiliensis by sequencing of 16S rRNA gene as a valuable method to identify clinical isolates. Combination therapy was performed by entire surgical excisions and intravenous TMP-SMX (160/800 mg/bid; IV) and meropenem 1g/tid. The patient's condition improved after 8 days and he was discharged. Conclusions: Combination therapy with surgical excision has a synergistic effect and appears to be the best treatment for extended lesions. However, new potent antibacterial drugs may help to improve the management of such infections when there are sufficient data on their in vitro activity.

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“…According to the difference in the susceptibility pattern of each member of the Nocardia asteroides complex, it can be concluded that prescription of effective antibiotics should be after the accurate identification of the causative Nocardia species. Also, clinical studies showed that some Nocardia species such as Nocardia otitidiscaviarum and Nocardia beijingensis could cause systematic infections, while other species like Nocardia brasiliensis cause limited local infections [1,[6][7]. Trimethoprim-sulfamethoxazole, amikacin, and imipenem are usually the first drug choices for nocardiosis [7].…”

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confidence: 99%

“…Also, clinical studies showed that some Nocardia species such as Nocardia otitidiscaviarum and Nocardia beijingensis could cause systematic infections, while other species like Nocardia brasiliensis cause limited local infections [1,[6][7]. Trimethoprim-sulfamethoxazole, amikacin, and imipenem are usually the first drug choices for nocardiosis [7]. However, the rate of fatality among patients with pulmonary and brain abscess who consumed trimethoprim-sulfamethoxazole ranged from 20 to 50% [8].…”

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confidence: 99%

Why Is The Identification of Causative Agent of Nocardiosis Essential at The Species Level?

Keikha

2017

JoMMID

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Molecular Identification and Susceptibility Pattern of Clinical Nocardia Species: Emergence of Nocardia crassostreae as an Agent of Invasive Nocardiosis

Cited by 13 publications

References 49 publications

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

Assessment of the Health Status of Mussels Mytilus galloprovincialis Along the Campania Coastal Areas: A Multidisciplinary Approach

Disseminated Nocardiosis in an Immunocompetent Individual Infected with Nocardia brasiliensis, an Opportunistic Agent

Why Is The Identification of Causative Agent of Nocardiosis Essential at The Species Level?

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