Molecular Identification and Susceptibility ofTrichosporonSpecies Isolated from Clinical Specimens in Qatar: Isolation ofTrichosporon dohaenseTaj-Aldeen, Meis & Boekhout sp. nov

Abstract

Trichosporon species have been reported as emerging pathogens and usually occur in severely immunocompromised patients. In the present work, 27 clinical isolates of Trichosporon species were recovered from 27 patients. The patients were not immunocompromised, except for one with acute myeloid leukemia. Sequence analysis revealed the isolation of Trichosporon dohaense Taj

Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

“…The automated commercially available Vitek 2 system identified the majority of isolates only to the genus level. Additionally, 27 of 51 isolates of basidiomycetous arthroconidial yeasts were identified previously by molecular methods (15). The remaining isolates were analyzed by ITS and LSU bar-coding during this study.…”

“…Use of the automated Vitek 1 and 2 systems (bioMérieux) has increased lately, but the Vitek 2 database includes only three Trichosporon and three Geotrichum species (17)(18)(19). Consequently, identifications usually are unreliable (15,17,18,20).…”

“…(16). Especially for uncommon species of Trichosporon and Geotrichum, identification at the species level is difficult to accomplish using commercially available assimilation tests and kits, such as the API 20 C AUX (bioMérieux), ID 32C (bioMérieux), and RapID Yeast Plus (Innovative Diagnostic Systems) systems (15,17). Use of the automated Vitek 1 and 2 systems (bioMérieux) has increased lately, but the Vitek 2 database includes only three Trichosporon and three Geotrichum species (17)(18)(19).…”

“…are azole resistant, while Trichosporon spp. are resistant to echinocandins), which affects proper disease treatment and patient care (15). Conclusive identification of pathogens should be fast and reliable to support the appropriate choice and application of antifungal therapy, adjustment of prophylactic treatment, and monitoring of the effects of treatment and the emergence of drug resistance.…”

Genotypic Analysis of a New Fungal Pathogen, Trichosporon faecale, Isolated from Japanese Subjects

“…Trichosporon faecale is an environmental fungus found mainly in soil [4]. Although T. faecale is generally considered to be non-pathogenic, it has been isolated from clinical specimens in Argentina [11], Brazil [12,13], Egypt [14], Qatar [14], Turkey [15], Spain [11], and Taiwan [16]. In the present study, we found that T. faecale was part of the fungal skin microbiome in Japanese subjects and that the genotype of the microorganism was of a single type.…”