Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

Zhang, Yanan; Ma, Ji-Fang; Sun, Liang; Dong, Zimei; Li, Zhaoqun; Zhu, Xiu-Yun; Wang, Yi; Wang, Lu; Deng, Daogui; Li, Jinbu

doi:10.1016/j.aspen.2016.05.009

“…Based on our previous antennal transcriptomic data (NCBI-SRX number: 2543665) for A. lepigone (Zhang et al, 2016b), we first identified 28 genes encoding putative OBPs including three PBP s and two GOBP s (Table 1). Among the 28 AlOBP s, 24 sequences were predicted to be full-length genes that encoded 133 to 246 amino acids; all 24 genes had a predicted signal peptide at the N-terminus.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we first identified 28 and 20 genes encoding putative AlOBPs and AlCSPs, respectively, based on our previous A. lepigone transcriptomic data (Zhang et al, 2016b). The number of AlOBP and AlCSP genes identified for this species are similar to some reported moths, such as C. suppressalis (Cao et al, 2014), H. armigera (Liu et al, 2012), and B. mori (Gong et al, 2007), but there are certain different from S. litura (Gu et al, 2015), S. inferens (Zhang et al, 2013), H. armigera (Liu et al, 2012) and B. mori (Gong et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…However, there are no reports on the chemosensory mechanism mediated by OBPs/CSPs between the pests and host plants. In this study, we identified 28 and 20 genes encoding putative AlOBPs ( A. lepigone OBPs) and AlCSPs ( A. lepigone CSPs), respectively, based on our previous transcriptomic data of A. lepigone (Zhang et al, 2016b). Tissue expression and phylogenetic analyses were conducted in an effort to postulate the function of these genes.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

Zhang

¹

,

Zhu

²

,

Ma

³

et al. 2017

PeerJ

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The olfaction system of insects plays an important role in mediating various physiological behaviors, including locating hosts, avoiding predators, and recognizing mates and oviposition sites. Therefore, some key genes in the system present valuable opportunities as targets for developing novel green pesticides. Athetis lepigone, a noctuid moth can feed on more than 30 different host plants making it a serious polyphagous pest worldwide, and it has become one of the major maize pests in northern China since 2011. However, there are no reports on effective and environmentally friendly pesticides for the control of this pest. In this study, we identified 28 genes encoding putative odorant binding proteins (OBPs) and 20 chemosensory protein (CSPs) genes based on our previous A. lepigone transcriptomic data. A tissue expression investigation and phylogenetic analysis were conducted in an effort to postulate the functions of these genes. Our results show that nearly half (46.4%) of the AlOBPs exhibited antennae-biased expression while many of the AlCSPs were highly abundant in non-antennal tissues. These results will aid in exploring the chemosensory mechanisms of A. lepigone and developing environmentally friendly pesticides against this pest in the future.

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“…Our previous studies have identified 66 ORs, 21 IRs, 33 OBPs, 24 CSPs, and 2 SNMPs mainly in antenna through transcriptome sequencing, and Xu et al (2016) reported 197 GRs based on the genome and transcriptome sequencing ( Liu et al, 2012 ; Liu N.Y. et al, 2014 ; Li et al, 2015 ; Zhang et al, 2015 ; Xu et al, 2016 ; Chang et al, 2017 ). Abundant chemosensory genes have been identified in the antennal transcriptome of numerous insects ( Gong et al, 2007 ; Grosse-Wilde et al, 2011 ; Bengtsson et al, 2012 ; Zhang et al, 2015 , 2016 ; Wang et al, 2017 ), but systematic gene excavation in mouthpart organs has not been done. Therefore, we were interested in determining how many types of chemosensory sensilla are in mouthpart organs and whether the mouthpart organs express abundant chemosensory proteins as in the antennae.…”

Section: Introductionmentioning

confidence: 99%

Chemoreception of Mouthparts: Sensilla Morphology and Discovery of Chemosensory Genes in Proboscis and Labial Palps of Adult Helicoverpa armigera (Lepidoptera: Noctuidae)

Guo

¹

,

Chen

²

,

Liu

³

et al. 2018

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Siphoning mouthparts, consisting of proboscis and labial palps, are the exclusive feeding organs and important chemosensory organs in most adult Lepidoptera. In this study, the general morphology of the mouthpart organs and precision architecture of the proboscis was described in adult Helicoverpa armigera. Three major sensilla types with nine subtypes including three novel subtypes were identified. The novel sensilla styloconica subtype 2 was the only one having a multiporous structure, which may play olfactory roles. For further understanding of the chemosensory functions of mouthpart organs, we conducted transcriptome analysis on labial palps and proboscises. A total of 84 chemosensory genes belonging to six different families including 4 odorant receptors (ORs), 6 ionotropic receptors (IRs), 7 gustatory receptors (GRs), 39 odorant binding proteins (OBPs), 26 chemosensory proteins (CSPs), and 2 sensory neuron membrane proteins (SNMPs) were identified. Furthermore, eight OBPs and six CSPs were identified as the novel genes. The expression level of candidate chemosensory genes in the proboscis and labial palps was evaluated by the differentially expressed gene (DEG) analysis, and the expression of candidate chemosensory receptor genes in different tissues was further investigated by quantitative real-time PCR (qRT-PCR). All the candidate receptors were detected by DEG analysis and qRT-PCR, but only a small part of the OR or IR genes was specifically or partially expressed in proboscis or labial palps, such as HarmOR58 and HarmIR75p.1, however, most of the GRs were abundantly expressed in proboscis or labial palps. The reported CO2 receptors such as HarmGR1, GR2, and GR3 were mainly expressed in labial palps. HarmGR5, GR6, and GR8, belonging to the “sugar receptor” clade, were mainly expressed in proboscis or antenna and were therefore suggested to perceive saccharide. The results suggest that the mouthparts are mutually cooperative but functionally concentrated system. These works contribute to the understanding of chemical signal recognition in mouthpart organs and provide the foundation for further functional studies.

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“…lepigone (Zhang et al 2016b), we first identified 28 genes encoding putative OBPs including three PBPs and two GOBPs (Table 1). Among the 28 AlOBPs, 24 sequences were predicted to be full-length genes that encoded 133 to 246 amino acids; all 24 genes had a predicted signal peptide at the N-terminus.…”

Section: Identification Of Putative Obp Genes In a Lepigonementioning

confidence: 99%

Peer Review #1 of "Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes in Athetis lepigone (Lepidoptera: Noctuidae) (v0.1)"

2017

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View full text Add to dashboard Cite

The olfaction system of insects plays an important role in mediating various physiological behaviors, including locating hosts, avoiding predators, and recognizing mates and oviposition sites. Therefore, some key genes in the system present valuable opportunities as targets for developing novel green pesticides. Athetis lepigone, a noctuid moth can feed on more than 30 different host plants making it a serious polyphagous pest worldwide, and it has become one of the major maize pests in northern China since 2011. However, there are no reports on effective and environmentally friendly pesticides for the control of this pest. In this study, we identified 28 genes encoding putative odorant binding proteins (OBPs) and 20 chemosensory protein (CSPs) genes based on our previous A. lepigone transcriptomic data. A tissue expression investigation and phylogenetic analysis were conducted in an effort to postulate the functions of these genes. Our results show that nearly half (46.4%) of the AlOBPs exhibited antennae-biased expression while many of the AlCSPs were highly abundant in non-antennal tissues. These results will aid in exploring the chemosensory mechanisms of A. lepigone and developing environmentally friendly pesticides against this pest in the future.

show abstract

Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

Cited by 15 publications

References 65 publications

Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

Chemoreception of Mouthparts: Sensilla Morphology and Discovery of Chemosensory Genes in Proboscis and Labial Palps of Adult Helicoverpa armigera (Lepidoptera: Noctuidae)

Peer Review #1 of "Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes in Athetis lepigone (Lepidoptera: Noctuidae) (v0.1)"

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