Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China

Xiao, Xiao; Qi, Rui; Han, Hui-Ju; Liu, Jianwei; Qin, Xiang-Rong; Fang, Li-Zhu; Zhou, Chunkui; Gong, Xiaoqing; Lei, Si-Cong; Yu, Xue‐Jie

doi:10.1016/j.ijppaw.2019.10.001

Cited by 13 publications

(7 citation statements)

References 42 publications

(58 reference statements)

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“…Previous studies have shown that carnivorous snakes and lizards infected with Cryptosporidium oocysts, possibly from the ingestion of infected rodents, may be asymptomatic [ 70 ]. Furthermore, predator–prey transmission of Cryptosporidium may occur, with Cryptosporidium -infected food maintaining a source of oocysts that are passively transferred through snakes [ 71 ]. As such, Cryptosporidium oocysts can be detected in lizards and snakes even when they are not infected.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing

Zeng

Xiong

Yang

et al. 2022

Animals

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The highly endangered crocodile lizard (Shinisaurus crocodilurus) continues to be impacted by disease, especially in captive breeding populations. In this paper, based on high-throughput sequencing, we investigated parasitic infections in captive and wild crocodile lizard populations in the Daguishan National Nature Reserve and Guangdong Luokeng Shinisaurus crocodilurus National Nature Reserve. The results show that the overall parasitic infection rate in crocodile lizards was 33.33% (23/69). Four parasite genera were detected, including Eimeria, Cryptosporidium, Nematopsis, and Acanthamoeba, with infection rates of 15.94% (11/69), 17.39% (12/69), 7.25% (5/69), and 4.35% (3/69), respectively. Significant differences in the infection rate were found between the different parasite species (χ2 = 8.54, p < 0.05, chi-squared test). The parasitic infection rates in the captive and wild populations were 39.29% (22/56) and 7.69% (1/13), respectively, which were significantly different (p < 0.05, Fisher’s exact test). However, no significant differences in the infection rates of the four parasite genera were found between the captive and wild populations (p > 0.05, Fisher’s exact test). The parasitic infection rates in Daguishan and Luokeng were 34.09% (15/44) and 32.00% (8/25), respectively, which were not significantly different (p > 0.05, Fisher’s exact test). However, significant differences in terms of species were found in the two reserves (p < 0.01, Fisher’s exact test). Only Cryptosporidium infection showed a significant difference between the two regions (p < 0.01, Fisher’s exact test). Our results suggest that captive crocodile lizards are more susceptible to parasitic diseases than wild crocodile lizards and that Cryptosporidium infection varies by geographical region. This study provides basic information about the parasites of endangered crocodile lizards, as well as a reference for disease control and conservation.

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Section: Discussionmentioning

confidence: 99%

Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing

Zeng

Xiong

Yang

et al. 2022

Animals

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“…Species identification was performed by nested PCR targeting the SSU rRNA gene with outer primers SSU‐F2 and SSU‐R2 and the inner primers SSU‐F3 and SSU‐R3 after Xiao et al. ( 2019 ). Genotyping relied on the protocol originally described by 2003 targeting the gp60 gene.…”

Section: Methodsmentioning

confidence: 99%

“…DNA samples identified as positive for Cryptosporidium by real-time PCR were subjected to species identification and genotyping. Species identification was performed by nested PCR targeting the SSU rRNA gene with outer primers SSU-F2 and SSU-R2 and the inner primers SSU-F3 and SSU-R3 after Xiao et al (2019). Genotyping relied on the protocol originally described by 2003 targeting the gp60 gene.…”

Section: Subtyping Of Giardia and Cryptosporidiummentioning

confidence: 99%

Zoonotic pathogens in wild muskoxen (Ovibos moschatus) and domestic sheep (Ovis aries) from Greenland

Berg

Stensvold

Jokelainen

et al. 2021

Veterinary Medicine & Sci

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The present study aimed to estimate the prevalence of zoonotic pathogens Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Erysipelothrix in muskoxen (Ovibos moschatus) and sheep (Ovis aries) from Greenland. In 2017 and 2018, faecal samples were collected from wild muskoxen from three distinct populations (Zackenberg, Kangerlussuaq, and Ivittuut) and from domestic sheep from southwest Greenland.Blood samples were collected from muskoxen from Kangerlussuaq and Ivittuut and from sheep. Faecal samples were tested for specific DNA of G. duodenalis and Cryptosporidium spp., and blood samples were tested for antibodies against T. gondii and Erysipelothrix. The estimated prevalence of G. duodenalis was 0% (0/58), 17% (7/41) and 0% (0/55) in muskoxen from Zackenberg, Kangerlussuaq and Ivittuut, respectively, and 37% (16/43) in sheep. The estimated prevalence of Cryptosporidium was 0% (0/58), 2%(1/41), 7% (4/55) in muskoxen from Zackenberg, Kangerlussuaq, Ivittuut, respectively, and 2% (1/43) in sheep. Neither Giardia nor Cryptosporidium were detected in winter samples (0/78). Of the positive samples, Giardia from one muskox sample only was successfully typed as G. duodenalis assemblage A, and Cryptosporidium from two muskoxen was successfully typed as C. parvum, subtype IIdA20G1e. The estimated T. gondii seroprevalence was 2% (1/44) and 0% (0/8) in muskoxen from Kangerlussuaq and Ivittuut, respectively, and 1% (1/155) in sheep. The estimated Erysipelothrix seroprevalence was 2% (1/45) and 13% (1/8) in muskoxen from Kangerlussuaq and Ivittuut, respectively, and 7% (10/150) in sheep. The results of this study add to the scarce knowledge on zoonotic pathogens in the Arctic.

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“…Currently, Cryptosporidium has been identified from domestic mammals ( 7 ), birds ( 8 ), reptiles ( 9 ), amphibians ( 10 ), and fishes ( 11 ). More than 40 species of Cryptosporidium have been identified ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Cryptosporidium spp. in Brandt's Vole in China

et al. 2020

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Cryptosporidium spp. are important intestinal parasites that infect humans and various animals, including wildlife. Currently, few epidemiological data in wild rodents, especially in voles, are available. In the present study, a total of 678 Brandt's vole feces samples were collected from Maodeng Livestock Farm and East Ujimqin, Inner Mongolia. The overall prevalence of Cryptosporidium spp. was 18.7%. Significant differences were not found between genders but between locations and weight groups. Moreover, three known species/genotypes, C. suis, Cryptosporidium environmental sequence and muskrat genotype II, and a novel Cryptosporidium species/genotypes of Brandt's vole was identified. To the best of our knowledge, this is the first report of Cryptosporidium spp. infection in Brandt's vole worldwide. These findings imply Brandt's voles might be a potential source of human cryptosporidiosis.

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Molecular identification and phylogenetic analysis of Cryptosporidium, Hepatozoon and Spirometra in snakes from central China

Cited by 13 publications

References 42 publications

Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing

Investigation of Parasitic Infection in Crocodile Lizards (Shinisaurus crocodilurus) Using High-Throughput Sequencing

Zoonotic pathogens in wild muskoxen (Ovibos moschatus) and domestic sheep (Ovis aries) from Greenland

Molecular Characterization of Cryptosporidium spp. in Brandt's Vole in China

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