Molecular identification and phylogenetic analysis of a viral RNA associated with the Chinese tobacco bushy top disease complex

Mo, Xiaohan; Chen, Z.-B.; Chen, J.-P.

doi:10.1111/j.1744-7348.2010.00452.x

Cited by 19 publications

(22 citation statements)

References 20 publications

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“…4 b). Virions were purified from C. frutescens leaves using the method described previously [ 17 ]. Isometric particles about 25 nm in diameter were observed in the purified preparation from the inoculated plants (Fig.…”

Section: Main Textmentioning

confidence: 99%

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Zhao

Yin

Hua

et al. 2021

Virol J

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Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan province, China. The complete genome sequence of a virus from a sample of these plants was determined by next-generation sequencing and RT-PCR. Pod pepper vein yellows virus (PoPeVYV) (MT188667) has a genome of 6015 nucleotides, and the characteristic genome organization of a member of the genus Polerovirus. In the 5′ half of its genome (encoding P0 to P4), PoPeVYV is most similar (93.1% nt identity) to PeVYV-3 (Pepper vein yellows virus 3) (KP326573) but diverges greatly in the 3′-part encoding P5, where it is most similar (91.7% nt identity) to tobacco vein distorting virus (TVDV, EF529624) suggesting a recombinant origin. Recombination analysis predicted a single recombination event affecting nucleotide positions 4126 to 5192 nt, with PeVYV-3 as the major parent but with the region 4126–5192 nt derived from TVDV as the minor parent. A full-length clone of PoPeVYV was constructed and shown to be infectious in C. frutescens by RT-PCR and the presence of icosahedral viral particles.

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Section: Main Textmentioning

confidence: 99%

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Zhao

Yin

Hua

et al. 2021

Virol J

View full text Add to dashboard Cite

show abstract

“…Virions were puri ed from C. frutescens leaves using the method described previously [17]. Isometric particles about 25nm in diameter were observed in the puri ed preparation from the inoculated plants (Fig.…”

Section: Main Textmentioning

confidence: 99%

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Zhao

Yin

Hua

et al. 2021

Preprint

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Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan province, China. The complete genome sequence of a virus from a sample of these plants was determined by next-generation sequencing and RT-PCR. Pod pepper vein yellows virus (PoPeVYV) (MT188667) has a genome of 6015 nucleotides, and the characteristic genome organization of a member of the genus Polerovirus. In the 5’ half of its genome (encoding P0 to P4), PoPeVYV is most similar (93.1% nt identity) to PeVYV-3 (Pepper vein yellows virus 3) (KP326573) but diverges greatly in the 3’-part encoding P5, where it is most similar (91.7% nt identity) to tobacco vein distorting virus (TVDV, EF529624) suggesting a recombinant origin. Recombination analysis predicted a single recombination event affecting nucleotide positions 4126 to 5192 nt, with PeVYV-3 as the major parent but with the region 4126-5192 nt derived from TVDV as the minor parent. A full-length clone of PoPeVYV was constructed and shown to be infectious in C. frutescens by RT-PCR and the presence of icosahedral viral particles.

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“…3B). Virions were puri ed from C. frutescens leaves using the method described previously [11]. Isometric particles about 25nm in diameter were observed in the puri ed preparation from the inoculated plants ( Fig.…”

Section: Main Textmentioning

confidence: 99%

Pod Pepper Vein Yellows Virus, a New Recombinant Polerovirus Infecting Pod Pepper in Yunnan Province, China

Zhao¹,

Yin²,

Hua³

et al. 2020

Preprint

View full text Add to dashboard Cite

Pepper vein yellows viruses (PeVYV) are phloem-restricted viruses in the genus Polerovirus, family Luteoviridae. Typical viral symptoms of PeVYV including interveinal yellowing of leaves and upward leaf curling were observed in pod pepper plants (Capsicum frutescens) growing in Wenshan city, Yunnan province, China. The complete genome sequence of a virus from a sample of these plants was determined by next-generation sequencing and RT-PCR. Pod pepper vein yellows virus (PoPeVYV) (MT188667) has a genome of 6015 nucleotides, and the characteristic genome organization of a member of the genus Polerovirus. In the 5’ half of its genome (encoding P0 to P4), PoPeVYV is most similar (94% nt identity) to PeVYV-3 (Pepper vein yellows virus 3) (KP326573) but diverges greatly in the 3’-part encoding P5, where it is most similar (88.8% nt identity) to tobacco vein distorting virus (TVDV, EF529624) suggesting a recombinant origin. Recombination analysis predicted a single recombination event at nucleotide positions 4126 to 5192 nt, with TVDV and PeVYV-3 as the minor and major parents, respectively. A full-length clone of PoPeVYV was constructed and shown to be infectious in C. frutescens by RT-PCR and the presence of icosahedral viral particles.

show abstract

Molecular identification and phylogenetic analysis of a viral RNA associated with the Chinese tobacco bushy top disease complex

Cited by 19 publications

References 20 publications

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Pod pepper vein yellows virus, a new recombinant polerovirus infecting Capsicum frutescens in Yunnan province, China

Pod Pepper Vein Yellows Virus, a New Recombinant Polerovirus Infecting Pod Pepper in Yunnan Province, China

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