Molecular identification and immunohistochemical localization of atrial natriuretic peptide in the heart of the dromedary camel (Camelus dromedarius)

Osman, A. H. K.; Yuge, Shinya; Hyodo, Susumu; Sato, Shigeaki; Maeda, Sakan; Marie, Hannon Anne; Caceci, Thomas; Birukawa, Naoko; Urano, Akihisa; Naruse, Kiyoko; Naruse, Mitsuhide; Takei, Yoshiyuki

doi:10.1016/j.cbpb.2004.05.009

Cited by 11 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the event, we found that both plasma and extracts of camel heart diluted in parallel with porcine BNP when using an antibody produced against porcine BNP. Our observations in this regard are congruent with an earlier study of camel natriuretic peptide cDNA which concluded that camel BNP has 94% identity with porcine BNP [8]. Our additional observation, using size-exclusion HPLC is that, as in many species including sheep [11], the dominant form of immunoreactive BNP in camel heart is a large molecular weight form consistent with the proBNP hormone - as we had hypothesized.…”

Section: Discussionsupporting

confidence: 91%

“…This indicates that immunoreactive BNP in the camel heart is in the form of proBNP with an approximate molecular weight of 11,600 Da, and close to the theoretical molecular weight of camel proBNP (11,734 Da) calculated from the dromedary sequence provided by Osman et al and in Genebank:BAD21300.1 [8]. Further, it is evident that little or no immunoreactive BNP is stored as smaller molecular weight BNP or NT-proBNP.…”

Section: Resultssupporting

confidence: 78%

See 1 more Smart Citation

ANP and BNP Responses to Dehydration in the One-Humped Camel and Effects of Blocking the Renin-Angiotensin System

et al. 2013

View full text Add to dashboard Cite

The objectives of this study were to investigate and compare the responses of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) in the circulation of hydrated, dehydrated, and dehydrated losartan - treated camels; and to document the cardiac storage form of B-type natriuretic peptide in the camel heart. Eighteen male camels were used in the study: control or hydrated camels (n = 6), dehydrated camels (n = 6) and dehydrated losartan-treated camels (n = 6) which were dehydrated and received the angiotensin II (Ang II) AT-1 receptor blocker, losartan, at a dose of 5 mg/kg body weight intravenously for 20 days. Control animals were supplied with feed and water ad-libitum while both dehydrated and dehydrated-losartan treated groups were supplied with feed ad-libitum but no water for 20 days. Compared with time-matched controls, dehydrated camels exhibited a significant decrease in plasma levels of both ANP and BNP. Losartan-treated camels also exhibited a significant decline in ANP and BNP levels across 20 days of dehydration but the changes were not different from those seen with dehydration alone. Size exclusion high performance liquid chromatography of extracts of camel heart indicated that proB-type natriuretic peptide is the storage form of the peptide.We conclude first, that dehydration in the camel induces vigorous decrements in circulating levels of ANP and BNP; second, blockade of the renin-angiotensin system has little or no modulatory effect on the ANP and BNP responses to dehydration; third, proB-type natriuretic peptide is the storage form of this hormone in the heart of the one-humped camel.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Resultssupporting

confidence: 78%

ANP and BNP Responses to Dehydration in the One-Humped Camel and Effects of Blocking the Renin-Angiotensin System

et al. 2013

View full text Add to dashboard Cite

show abstract

“…However, hydration/dehydrationdependent processes may also play a role, as suggested by the fluctuations in cardiac ANP content and plasma levels experienced by water-deficiency-tolerant mammals. In the desert rat (Lacas et al, 1998;Lacas et al, 2000) and the dromedary camel (Osman et al, 2004), NP-dependent mechanisms significantly contribute to coping with these environmental challenges. Notably, in the camel, intensive rehydration after a long period of water deprivation does not increase blood volume, the water being first stored in the rumen-like forestomach and then directed to the interstitium.…”

Section: Np From Ontogeny To Physio-pathology: the Mammalian Lessonmentioning

confidence: 99%

Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a ‘whip-brake’ system of the endocrine heart

Tota

Cerra

Gattuso

2010

Journal of Experimental Biology

View full text Add to dashboard Cite

Summary In the past 50 years, extensive evidence has shown the ability of vertebrate cardiac non-neuronal cells to synthesize and release catecholamines (CA). This formed the mindset behind the search for the intrinsic endocrine heart properties, culminating in 1981 with the discovery of the natriuretic peptides (NP). CA and NP, co-existing in the endocrine secretion granules and acting as major cardiovascular regulators in health and disease, have become of great biomedical relevance for their potent diagnostic and therapeutic use. The concept of the endocrine heart was later enriched by the identification of a growing number of cardiac hormonal substances involved in organ modulation under normal and stress-induced conditions. Recently, chromogranin A (CgA), a major constituent of the secretory granules, and its derived cardio-suppressive and antiadrenergic peptides, vasostatin-1 and catestatin, were shown as new players in this framework, functioning as cardiac counter-regulators in ‘zero steady-state error’ homeostasis, particularly under intense excitatory stimuli, e.g. CA-induced myocardial stress. Here, we present evidence for the hypothesis that is gaining support, particularly among human cardiologists. The actions of CA, NP and CgA, we argue, may be viewed as a hallmark of the cardiac capacity to organize ‘whip-brake’ connection-integration processes in spatio-temporal networks. The involvement of the nitric oxide synthase (NOS)/nitric oxide (NO) system in this configuration is discussed. The use of fish and amphibian paradigms will illustrate the ways that incipient endocrine-humoral agents have evolved as components of cardiac molecular loops and important intermediates during evolutionary transitions, or in a distinct phylogenetic lineage, or under stress challenges. This may help to grasp the old evolutionary roots of these intracardiac endocrine/paracrine networks and how they have evolved from relatively less complicated designs. The latter can also be used as an intellectual tool to disentangle the experimental complexity of the mammalian and human endocrine hearts, suggesting future investigational avenues.

show abstract

“…Gower et al [15] and Vuolteenaho et al [16] demonstrated that the gene for ANP was expressed in specific regions of the rat gastrointestinal tract. It has been found immunohisto-chemically that EC cells in the antral and small and large intestinal mucosa, besides being serotonin positive, are also ANP positive [7,8] .…”

Section: Discussionmentioning

confidence: 99%

Relationship between atrial natriuretic peptide-immunoreactive cells and microvessels in rat gastric mucosa1

Yang

Yin

et al. 2006

Acta Pharmacologica Sinica

View full text Add to dashboard Cite

Aim: To investigate the ultrastructural localization of atrial natriuretic peptide (ANP)-synthesizing cells and the relationship between ANP-synthesizing cells and microvessels in rat gastric mucosa. Methods: Immunohistochemistry techniques and postembedding immunoelectron microscopy techniques were used to validate the findings regarding the expression of ANP-synthesizing cells and the ultrastructural localization of ANP-synthesizing cells in the gastric mucosa. Histochemistry techniques and the tannic acid-ferric chloride method (TA-Fe staining method) were used to reveal microvessel density and the distribution of ANPsynthesizing cells in different regions of the stomach. Results: Cells expressing ANP were localized and ANP-synthesizing cells were identified as enterochromaffin (EC) cells in the gastric mucosa. ANP-synthesizing cells existed in different regions of the stomach. The percentage ANP-synthesizing cells in the mucosa was greatest in the fundus (46.7%±5.3%), intermediate in the antrum (40.1%± 4.5%), and least in the body (21.6%±3.6%). There was a positive relationship between the percentage of ANP-synthesizing cells and the density of microvessels in the antral mucosa, but not in the fundus or body mucosa. Conclusion: ANP is synthesized by EC cells in rat gastric mucosa, and ANP-synthesizing cells are most dense in the gastric fundus. ANP may act not only as a regional autocrine and/or paracrine regulator, but also as an endocrine regulatory peptide in the gastrointestinal tract.

show abstract

Molecular identification and immunohistochemical localization of atrial natriuretic peptide in the heart of the dromedary camel (Camelus dromedarius)

Cited by 11 publications

References 33 publications

ANP and BNP Responses to Dehydration in the One-Humped Camel and Effects of Blocking the Renin-Angiotensin System

ANP and BNP Responses to Dehydration in the One-Humped Camel and Effects of Blocking the Renin-Angiotensin System

Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a ‘whip-brake’ system of the endocrine heart

Relationship between atrial natriuretic peptide-immunoreactive cells and microvessels in rat gastric mucosa1

Contact Info

Product

Resources

About