Molecular identification and genotyping of Enterocytozoon bieneusi in experimental rats in China

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

“…To the best of our knowledge, this is the rst report of E. bieneusi infections in coypus in China. In the present study, the infection rate of E. bieneusi was 41.2% in coypus, which is higher than the infection rate of E. bieneusi reported in brown rats (7.9%) [8], bamboo rats (5.1%) [15], experimental brown rats (4.8%) [16], commensal rodents (mouse and brown rat) (4.0%) [14], pet chinchillas (3.6%) [17] and red squirrels (19.4%) [18] in China. In addition, lower infection rates were also reported in wild house mice (10.7%) from a hybrid zone across the Czech Republic-Germany border [19], and beavers (15.3%) and muskrats (8.4%) from USA [20].…”

Host-Adaptation of Rare Enterocytozoon bieneusi Genotype CHN4 in Coypu (Myocastor Coypus) in China

“…Similar infection rates of E. bieneusi have been reported in small rodents (38.9%) from southwestern Poland [19], and a laboratory prairie dog colony (37.9%) from USA [20]. The infection rates of E. bieneusi in rodents could be in uenced by many factors, such as animal immune status, age distribution, sample size, detection method, feeding environment, management system and population density [15]. Although the high infection rate was detected in coypus in our study, we cannot come to an inference that coypus are more susceptible to E. bieneusi than many other rodent species due to the lack of more investigations.…”

“…To the best of our knowledge, this is the rst report of E. bieneusi infections in coypus in China. In the present study, the infection rate of E. bieneusi was 41.2% in coypu, which is higher than the infection rate of E. bieneusi reported in brown rats (7.9%) [10], bamboo rats (5.1%) [14], experimental rats (4.8%) [15], commensal rodents (4.0%) [13] and pet chinchillas (3.6%) in China [16]. In addition, lower infection rates were also reported in wild house mice (10.7%) from a hybrid zone across the Czech Republic-Germany border [17], and beavers (15.3%) and muskrats (8.4%) from USA [18].…”

“…Genotype D was identi ed in squirrels from China [23] and USA [12], chipmunks [24], bamboo rats [14] and brown rats [10,25] from China, house mice from Czech Republic-Germany border [17] and striped eld mice from Poland [19], and genotype WL4 was observed in squirrels, chipmunks and muskrats from USA [12,18] (Table 3). EbpA, EbpC, PigEBITS7, S7, Peru16 and CHG4 have also been reported as the most common genotypes [9,13,15,18,25,26]. The rare genotype CHN4 was the dominant genotype, indicating that the transmission dynamic of E. bieneusi in coypus is different from other rodents.…”

“…Genotypes EbpA and EbpC have been detected in several rodent species (squirrel, house mouse, experimental rat, muskrat, bamboo rat and beaver) worldwide [14,15,17,18,23] (Table 3). EbpA and EbpC are two of the most common genotypes detected in both immunocompetent and immunocompromised people worldwide [1].…”