Molecular identification and gene expression profiles of the T cell receptors and co-receptors in developing red-tailed phascogale (Phascogale calura) pouch young

Borthwick, Casey R.; Young, Lauren J.; Old, Julie M.

doi:10.1016/j.molimm.2018.07.005

Cited by 1 publication

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hassall's corpuscles can also be observed at this stage (Borthwick & Old, 2016). Expression of CD3ε, CD4, TCRδ, and TCRμ were detected on Day 1 postpartum in the thymus, whilst the remaining genes isolated by Borthwick et al (2018) were expressed from Day 3 postpartum.…”

Section: Red‐tailed Phascogalesmentioning

confidence: 90%

“…The thymus is responsible for the development and maturation of T‐cells in vertebrates, and thus required for specific immune defense (Miller, 2002). Borthwick et al (2018) identified and characterized phascogale T‐cell specific genes including CD3ε, CD4, CD8α, TCRα, TCRβ, TCRγ, TCRδ, and TCRμ. These genes in phascogales are likely to perform similar functions as those in other marsupials and eutherian mammals, thus enabling the use of phascogales in developmental studies of the immune system and particularly T‐cell function.…”

Section: Red‐tailed Phascogalesmentioning

confidence: 99%

“…Splenic rudiment amongst the mesentery were first observed at Day 5 in the phascogale pouch young (Borthwick & Old, 2016). Peak expression of mature T‐cell markers was observed around the time of initial red and white pulp differentiation (Borthwick et al, 2018). The phascogale gut resembled mainly small intestine on Day 1 after birth, however, by Day 2 postpartum the stomach was observed, and by Day 5 postpartum the small and large intestine was present (Borthwick & Old, 2016).…”

Section: Red‐tailed Phascogalesmentioning

confidence: 99%

“…As we now have an excellent description of the development of the immune tissues (Borthwick & Old, 2016; Old et al, 2006) and the appearance of some key immunological markers in the phascogale (Borthwick et al, 2018, 2019), we can now utilize the phascogale to investigate immunological questions. Given that male phascogales are semelparous (Bradley, 1987), they constitute an ideal model to use for the study of the impacts of physiological stress response and stress hormones on the neuroendocrine system.…”

Section: Red‐tailed Phascogalesmentioning

confidence: 99%

See 3 more Smart Citations

Red‐tailed phascogales: A review of their biology and importance as model marsupial species

2020

Self Cite

View full text Add to dashboard Cite

There are many limitations when using traditional laboratory species. Limits on variation, may result in limited outcomes, at both the species and individual level, due to different individuals/species having diverse physiological processes, or differing molecular and genetic mechanisms. By using a variety of model species, we will be able to develop creative solutions to biological problems and identify differences of which we were not previously aware. The laboratory mouse has been a suitable model species for various mammalian studies, however most are bred specifically for laboratory research with limited variability due to selective breeding. Marsupial models offer unique research opportunities compared to eutherian models. We believe that there should be an expansion in marsupial model species, and the introduction of the red‐tailed phascogale (Phascogale calura), a dasyurid marsupial, should be one of them. Phascogales are easily managed in captivity, and there are now multiple studies involving their development, reproduction, nutrition, behavior and immune system, which can serve as a baseline for future studies. The addition of the phascogale as a model species will improve future mammalian studies by introducing variability and offer alternate solutions to biological problems, particularly in the areas of genetics, nutrition, immunology, the neuro‐endocrine system, and ageing, due to their semelparous reproductive strategy and hence, subsequent predictive physiology. In this review, we provide information based on existing research on red‐tailed phascogales to support their inclusion as a model species.

show abstract

Section: Red‐tailed Phascogalesmentioning

confidence: 90%

Section: Red‐tailed Phascogalesmentioning

confidence: 99%