Molecular Identification and Expression Analysis of Carbonic Anhydrase VII in Pufferfish (Takifugu rubripes)

Sumi, Kanij Rukshana; Kim, Soo Cheol; Howlader, Jewel; Sharker, Rajib; Choi, Kap Seong; Choi, Soo Hyuk; Park, Jong-In; Nou, Ill–Sup; Kho, Kang Hee

doi:10.1007/s12601-019-0020-z

Cited by 2 publications

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References 33 publications

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“…H. discus hannai is considered a top-priced seafood item because it contains health beneficial bioactive molecules besides its basic nutritional value ( Suleria et al, 2017 ). Many studies regarding cytosolic CA genes have been performed in different teleosts, non-teleosts, cyclostomes, and mammals ( Murakami et al, 1987 ; Lund et al, 2002 ; Esbaugh et al, 2005 , 2009 ; Gilmour et al, 2007 ; Sumi et al, 2019 ). Two CAs have been cloned in the mantle tissue of European abalone, Haliotis tuberculata ( Le Roy et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization

Sharker

Kim

Hossen

et al. 2021

Front. Mol. Biosci.

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Carbonic anhydrases (CAs) are universal zinc ion containing metalloenzymes that play a pivotal role in various physiological processes. In this study, a CA I (designated as Hdh CA I) was isolated and characterized from the mantle tissue of Pacific abalone, Haliotis discus hannai. The full-length cDNA sequence of Hdh CA I was 1,417-bp in length, encoding a protein of 337 amino acids with molecular weight of 37.58 kDa. Hdh CA I sequence possessed a putative signal peptide of 22 amino acids and a CA catalytic function domain. The predicted protein shared 94 and 78% sequence identities with Haliotis gigantea and Haliotis tuberculata CA I, respectively. Results of phylogenetic analysis indicated that Hdh CA I was evolutionarily close to CA I of H. gigantea and H. tuberculata with high bootstrap values. Significantly higher levels of Hdh CA I mRNA transcript were found in mantle than other examined tissues. In situ hybridization results showed strong hybridization signals in epithelial cells of the dorsal mantle pallial, an area known to synthesize and secrete proteins responsible for the nacreous layer formation of shell. This is the first study on Hdh CA I in H. discus hannai and the results may contribute to further study its physiological functions in shell biomineralization of abalone.

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Section: Introductionmentioning

confidence: 99%

Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization

Sharker

Kim

Hossen

et al. 2021

Front. Mol. Biosci.

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“…Haliotis discus hannai is considered as a popular seafood item worldwide due to its contents of health beneficial bioactive molecules (Suleria et al, 2017). Previous studies have characterized cytosolic CA isozymes in vertebrates and invertebrates (Pongsomboon et al, 2009;Le Roy et al, 2012;Ali et al, 2015;Pan et al, 2016;Sumi et al, 2019). However, the characterization and expression analysis of CA isozymes in Pacific abalone have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Carbonic Anhydrase II (CA II) and Its Potential Involvement in Regulating Shell Formation in the Pacific Abalone, Haliotis discus hannai

Sharker

Sukhan

Sumi

et al. 2021

Front. Mol. Biosci.

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Carbonic anhydrases (CAs) are a family of metalloenzymes that can catalyze the reversible interconversion of CO2/HCO3–, ubiquitously present in both prokaryotes and eukaryotes. In the present study, a CA II (designated as HdhCA II) was sequenced and characterized from the mantle tissue of the Pacific abalone. The complete sequence of HdhCA II was 1,169 bp, encoding a polypeptide of 349 amino acids with a NH2-terminal signal peptide and a CA architectural domain. The predicted protein shared 98.57% and 68.59% sequence identities with CA II of Haliotis gigantea and Haliotis tuberculata, respectively. Two putative N-linked glycosylation motifs and two cysteine residues could potentially form intramolecular disulfide bond present in HdhCA II. The phylogenetic analysis indicated that HdhCA II was placed in a gastropod clade and robustly clustered with CA II of H. gigantea and H. tuberculata. The highest level of HdhCA II mRNA expression was detected in the shell forming mantle tissue. During ontogenesis, the mRNA of HdhCA II was detected in all stages, with larval shell formation stage showing the highest expression level. The in situ hybridization results detected the HdhCA II mRNA expression in the epithelial cells of the dorsal mantle pallial, an area known to express genes involved in the formation of a nacreous layer in the shell. This is the first report of HdhCA II in the Pacific abalone, and the results of this study indicate that this gene might play a role in the shell formation of abalone.

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Molecular Identification and Expression Analysis of Carbonic Anhydrase VII in Pufferfish (Takifugu rubripes)

Cited by 2 publications

References 33 publications

Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization

Carbonic Anhydrase in Pacific Abalone Haliotis discus hannai: Characterization, Expression, and Role in Biomineralization

Molecular Characterization of Carbonic Anhydrase II (CA II) and Its Potential Involvement in Regulating Shell Formation in the Pacific Abalone, Haliotis discus hannai

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