Molecular identification and distribution profile ofCandidaspecies isolated from Iranian patients

Abstract

A total of 855 yeast strains isolated from different clinical specimens, mainly nail (42%) and vulva-vagina (25%) were identified by a set of polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP). Genomic DNA was extracted from fresh colonies using Whatman FTA Card technology. PCR assays were performed on the complete ribosomal DNA internal transcribed spacer (rDNA-ITS) region for all isolates and species identification was carried out through their specific electrophoretic profiles after digestion with the enzyme MspI. Those isolates suspected as Candida parapsilosis group were then subjected to amplification of the secondary alcohol dehydrogenase (SADH) gene and restriction digestion with NlaIII enzyme. In total, 71.1% of the strains were obtained from females and 28.9% from males. The age group of 31-40 years consisted of the highest frequency of patients with candidiasis. Candida albicans was the predominant species (58.6%) followed by C. parapsilosis (11.0%), C. glabrata (8.3%), C. tropicalis (7.0%), C. kefyr (5.8%), C. krusei (4.4%), C. orthopsilosis (2.1%), and C. guilliermondii (0.6%). A few strains of C. lusitaniae, C. rugosa, C. intermedia, C. inconspicua, C. neoformans and S. cerevisiae were isolated. We could not identify 8 (0.9%) isolates. Candida albicans remains the most frequently species isolated from Iranian patients; however, the number of non-C. albicans Candida species looks to be increasing. The simple and reliable PCR-RFLP system used in the study has the potential to identify most clinically isolated yeasts.

Study of Skin and Nail Candida Species as a Normal Flora Based on Age Groups in Healthy Persons in Tehran-Iran (2016)

“…Isolation of C. parapsilosis as the commonest species (35%) in all sites of the skin in our study was noteworthy which was consist with other studys about candida colonization [13,14]. As other surveys that determined C. albicans seldom lives saprophitically on the skin [7] we found that C. albicans is one of the least inhabitants of the skin, whereas new studied showed among Candida species, C. albicans is still the most common infectious agent isolated from clinical samples in Iran and the word [13,15].…”

Molecular identification and antifungal susceptibility profiles of Candida parapsilosis complex species isolated from culture collection of clinical samples

“…However, C. orthopsilosis was not found. Mohammadi et al (35) have shown that C. parapsilosis sensu stricto is the dominant species, followed by C. orthopsilosis. In contrast, similar to our results, Ge et al (19) have found that C. parapsilosis sensu stricto and C. metapsilosis are the agents responsible for onychomycosis.…”

Microevolution of <i>Candida albicans</i> Isolate from a Patient with Mucocutaneous Candidiasis and HIV Infection

“…Mucocutaneous candidiasis, including oropharyngeal, oesophageal, and vulvovaginal forms, produced by C. albicans is the most common fungal infection in individuals with HIV [5]; however, the involvement of other Candida species has also been reported [3]. In this study, PCR-RFLP analysis of the 5.8S-ITS region clearly demonstrated C. albicans infection in the three analysed clinical samples.…”

“…has been standardised using different kits; however, these approaches are often limited by variations in the phenotypic expression of different Candida species. Alternative molecular methods have been developed, which are mostly based on the amplification of specific regions, such as the 5.8S-ITS region, followed by restriction analysis [5].…”