Molecular identification and characterization of partial COX1 gene from caecal worm (Aulonocephalus pennula) in Northern bobwhite (Colinus virginianus) from the Rolling Plains Ecoregion of Texas

Kalyanasundaram, Aravindan; Blanchard, Kendall R.; Kendall, Ronald J.

doi:10.1016/j.ijppaw.2017.07.002

Cited by 16 publications

(13 citation statements)

References 37 publications

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“…The mitochondrial COX1 and COX2 genes are widely applied to identify parasite species and to distinguish evolutionary relationships between parasites. This gene has been evaluated as a potential genetic marker in the phylum of parasites such as Aulonocephalus pennula , Contracaecum osculatum , and Cysticercus tenuicollis [ 49 , 50 ]. It has been documented that NO is toxic for T .…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

et al. 2018

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Trichinella spiralis mainly dwells in the muscle tissue of its host and is the main causative agent of trichinellosis in humans. Nitric oxide (NO), an important intracellular signaling molecule that may restrict pathogen growth in infected hosts, has been known for its anti-pathogenic activity, including resistance to T. spiralis. Herein, we applied label-free analysis to investigate the effect of sodium nitroprusside (SNP, a NO donor compound) on the proteome of T. spiralis muscle larvae (ML), followed by Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analyses. Of the 1,476 proteins detected in the ML, 121 proteins showed differential expression, including 50 significantly upregulated and 71 downregulated proteins. The functions of the 108 annotated proteins were primarily related to signal transduction, transcription/translation, material metabolism, protein synthesis/assembly/degradation, and stress/defense/antioxidation. Quantitative real-time polymerase chain reaction (qRT-PCR) assay verified that FRMD5 and CUT-1 gene expression levels were significantly increased, while COX2 gene expression level was significantly decreased. GO annotation and KEGG pathway analyses showed that the majority of differentially expressed proteins were mainly involved in the molecular function of the catalytic activity, biological process of the immune system process, metabolic process, cellular component organization, biological adhesion, and cellular component of the macromolecular complex. Our results demonstrate the first comprehensive protein expression profile of the ML in response to NO stress and provide novel references for understanding the potential mechanism underlying the effects of NO on trichinellosis.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

et al. 2018

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“…The mitochondrial gene cytochrome c oxidase subunit one ( COX1 ) has proved to be a useful molecular marker for the detection and identification of various parasites and pathogens, including, but not limited to, nematodes (Aravindan et al, 2017), trypanosomes (Rodrigues et al, 2017), ticks (Chitimia et al, 2010) and oestrid flies (Samuelsson et al, 2013). Additionally, this mtDNA marker has provided clear resolution on the relationships of the L. bufonivora species group: L. bufonivora , L. elongata and L. silvarum (McDonagh and Stevens, 2011; Arias-Robledo et al, 2019a).…”

Section: Introductionmentioning

confidence: 99%

Ecological and geographical speciation in Lucilia bufonivora: The evolution of amphibian obligate parasitism

Arias-Robledo

Wall

Szpila

et al. 2019

International Journal for Parasitology: Parasites and Wildlife

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Lucilia (Diptera: Calliphoridae) is a genus of blowflies comprised largely of saprophagous and facultative parasites of livestock. Lucilia bufonivora, however, exhibits a unique form of obligate parasitism of amphibians, typically affecting wild hosts. The evolutionary route by which amphibian myiasis arose, however, is not well understood due to the low phylogenetic resolution in existing nuclear DNA phylogenies. Furthermore, the timing of when specificity for amphibian hosts arose in L. bufonivora is also unknown. In addition, this species was recently reported for the first time in North America (Canada) and, to date, no molecular studies have analysed the evolutionary relationships between individuals from Eastern and Western hemispheres. To provide broader insights into the evolution of the amphibian parasitic life history trait and to estimate when the trait first arose, a time-scaled phylogeny was inferred from a concatenated data set comprising mtDNA, nDNA and non-coding rDNA (COX1, per and ITS2 respectively). Specimens from Canada, the UK, Poland, Switzerland, the Netherlands and Germany were analysed, as well as individuals from its sister taxa, the saprophage Lucilia silvarum and a Nearctic species also implicated in amphibian myiasis, Lucilia elongata. Obligate amphibian parasitism appears to have arisen ~4 mya, likely as a result of niche displacement of a saprophagous/facultative parasite ancestor. Consistent paraphyly of L. bufonivora with respect to L. elongata across single-gene phylogenies and high mtDNA genetic distances between Nearctic and Palearctic individuals suggest on-going cryptic speciation facilitated by geographical isolation. These findings suggest that recent reports of L. bufonivora in the Nearctic do not constitute a recent introduction, but instead suggest that it remained unrecorded due to taxonomic confusion and low abundance. This is the first study to confirm the involvement of L. bufonivora in amphibian myiasis in Canada using DNA-based identification methods.

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“…This PCR product was then sequenced to obtain the near complete 18S rDNA sequence. The partial COX1 sequence was confirmed with similar methods as described by Kalyanasundaram et al (2017) using Thelazia callipaeda COX1 sequence (Liu et al 2013) as a comparison. Raw sequences were trimmed using DNA chromatogram explorer (www.dnabaser.com).…”

Section: Sequencingmentioning

confidence: 86%

Phylogenetic analysis of eyeworm (Oxyspirura petrowi) in northern bobwhite (Colinus virginianus) based on the nuclear 18S rDNA and mitochondrial cytochrome oxidase 1 gene (COX1)

et al. 2018

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Oxyspirura petrowi is a heteroxenous nematode found in northern bobwhite (Colinus virginianus) of the Rolling Plains ecoregion of Texas. Despite its impact on this popular gamebird, genetic level studies on O. petrowi remain relatively unexplored. To accomplish this, we chose the previously studied nuclear rDNA 18S region as well as the mitochondrial COX1 gene region of O. petrowi to investigate phylogenetic relations between O. petrowi and other nematode species. In this study, we generate primers using multiple alignment and universal nematode primers to obtain a near-complete 18S and partial COX1 sequence of O. petrowi, respectively. Phylogenetic trees for O. petrowi's 18S and COX1 gene regions were constructed using the Maximum Likelihood and Maximum Parsimony method. A comparative analysis was done based on the nuclear and mitochondrial region similarities between O. petrowi and other nematode species that infect both humans and animals. Results revealed a close relation to the zoonotic eyeworm Thelazia callipaeda as well as a close relation with filarial super family (Filarioidea) such as the human eyeworm Loa loa and Dirofilaria repens eyeworm of dog and carnivores.

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Molecular identification and characterization of partial COX1 gene from caecal worm (Aulonocephalus pennula) in Northern bobwhite (Colinus virginianus) from the Rolling Plains Ecoregion of Texas

Cited by 16 publications

References 37 publications

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

Proteomic analysis of the response of Trichinella spiralis muscle larvae to exogenous nitric oxide

Ecological and geographical speciation in Lucilia bufonivora: The evolution of amphibian obligate parasitism

Phylogenetic analysis of eyeworm (Oxyspirura petrowi) in northern bobwhite (Colinus virginianus) based on the nuclear 18S rDNA and mitochondrial cytochrome oxidase 1 gene (COX1)

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