Molecular identification and characterization of<i>Fusarium</i>spp. associated with sorghum seeds

Divakara, S.; Parthasarathy, S.; Aiyaz, Mohammed; Ramana, M. Venkata; Hariprasad, P.; Nayaka, S. Chandra; Niranjana, S. R.

doi:10.1002/jsfa.6380

Cited by 45 publications

(50 citation statements)

References 40 publications

(31 reference statements)

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“…F. incarnatum was identified as a predominant (37 of the 59 isolates tested) species in Fusarium sorghum mold complex. This species has been associated with sorghum seeds in India (Sharma et al, 2011;Divakara et al, 2013) and Argentina (Gonzalez et al, 1997). The species of the Liseola section (F. verticillioides, F. thapsinum, F. proliferatum and F. pseudonygamai) with 10 isolates are also important.…”

Section: Fungusmentioning

confidence: 97%

“…The species of the Liseola section (F. verticillioides, F. thapsinum, F. proliferatum and F. pseudonygamai) with 10 isolates are also important. Fusarium species included in the section Liseola have been reported to be associated with sorghum grain mold complex in different countries (Divakara et al, 2013). In fact, F. thapsinum is the predominant species in India (Sharma et al, 2011) and Uganda (Prom et al, 2011), and has been reported to be common in sorghum grain (Leslie et al, 2005).…”

Section: Fungusmentioning

confidence: 99%

“…Da Silva et al (2006) studied the genetic variability of 21 Fusarium verticilloides strains isolated from sorghum cultivated in the State of São Paulo, Brazil, using SPAR (Single Primer Amplification Reaction) markers while Divakara et al (2013) identified and characterized 27 Fusarium spp., isolated from 58 sorghum seed samples collected across India, sequencing the transcription elongation factor 1 alpha gene and analyzing the Inter Simple Sequence Repeat (ISSR) polymorphisms. Additionally, Priyanka et al (2014) studied the diverse distribution of 200 Aspergillus strains from a total of 320 samples of maize, paddy, sorghum and groundnut by species-specific PCR assays.…”

Section: Fungusmentioning

confidence: 99%

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Toxigenic molds in Tunisian and Egyptian sorghum for human consumption

Lahouar

Crespo-Sempere

Marı́n

et al. 2015

Journal of Stored Products Research

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a b s t r a c tThe objective of this study was to characterize the mycoflora of sorghum grains commercialized in the Tunisian retail market and to identify aflatoxins (AFs), ochratoxin A (OTA) and zearalenone (ZEA) producing species. Sixty four samples of sorghum (37 samples of Tunisian sorghum and 27 samples of Egyptian sorghum) were analyzed. Dilution plating (CFU, colony forming units) was used for fungal enumeration. The isolation of mycobiota was carried out by plating of grains on PDA and malachite green medium. Aspergillus section Flavi and section Nigri and Fusarium isolates were sub-cultured in CYA to test their ability to produce AFs, OTA and ZEA, respectively. The selected Aspergillus section Flavi and section Nigri, Penicillium and Fusarium isolates were subjected to specific PCR assays using published speciesspecific primers. The results revealed the dominance of Fusarium (95.3%), followed by Aspergillus (87.2%) and Alternaria (81.2%) species. The fungal counts ranged from 100 to 1.3$10 4 CFU/g for Tunisian sorghum and from 100 to 5.7$10 3 CFU/g for Egyptian sorghum. Among Aspergillus section Flavi isolates identified by molecular biology, Aspergillus flavus was the most dominant (90.1%) while Aspergillus parasiticus represent 9.9% only. About Aspergillus section Nigri, results showed the dominance of Aspergillus niger aggregate species, including Aspergillus niger, Aspergillus tubingensis and other species. Among Fusarium species, Fusarium incarnatum was the most dominant in both Tunisian and Egyptian sorghum. Penicillium citrinum was the dominant Penicillium species in the studied samples. More than 890 isolates belonging to the genus Aspergillus and Fusarium were tested in order to test their capacity to produce AFs, OTA and ZEA. The percentage of mycotoxin producing isolates in Aspergillus section Flavi, A. section Nigri, and Fusarium was 30.0%, 4.6% and 11.1%, respectively.

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Section: Fungusmentioning

confidence: 97%

Section: Fungusmentioning

confidence: 99%

Section: Fungusmentioning

confidence: 99%

See 1 more Smart Citation

Toxigenic molds in Tunisian and Egyptian sorghum for human consumption

Lahouar

Crespo-Sempere

Marı́n

et al. 2015

Journal of Stored Products Research

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“…Similar observations have been made by many researchers on Fusarium spp. of other major food crops (Divakara et al, 2014). Four ISSR primers yielding highly polymorphic markers proved to be authentic and reliable for inferring the genetic relationships within and between Fusarium spp (Mishra et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…The sequences most commonly used to distinguish Fusarium spp. are portions of genomic sequences encoding translation elongation factor-1α (Divakara et al, 2014), β-tubulin (O'Donnell et al, 1998), calmodulin (O'Donnell et al, 2000, internal transcribed spacer regions in the ribosomal repeat region (ITS1 and ITS2) Gaikwad et al, 2013) and the intergenic spacer region (IGS) (Yli-Mattila et al, 2010). Eight isolates were capable of producing detectable levels of FB1, NIV and DON, although seventeen isolates failed to produce any detectable amount.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and characterization of Fusarium spp. associated with wheat grains

Mahmoud¹,

Shehata²

2017

Int. J. Adv. Res. Biol. Sci

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Thirty one isolates belonging to six fungal genera were found to be associated with wheat grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (19.52%), Fusarium (32.31%), Penicillium (12.19%), and Alternaria (8.2%). In this study, different isolates of Fusarium spp. were isolated from wheat grains samples and identified at the molecular level by ITS-rDNA regions amplification. Twenty five isolates of Fusarium spp. were screened for their ability to produce Fumonisin B1 (FB1), nivalenol (NIV) and deoxynivalenol (DON) using HPLC. Eight isolates were capable of producing detectable levels of FB1, NIV and DON. Inter-simple sequence repeats (ISSR) molecular markers were used, with the aim of genetically characterizing isolates of Fusarium spp. and discriminate between atoxigenic and toxigenic isolates. ISSR analysis revealed a high level of genetic diversity in the Fusarium spp. population and useful for genetic characterization. ISSR markers were not suitable to discriminate atoxigenic and toxigenic isolates also, Clustering based on ISSR dendrograms was unrelated to geographic origin.

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Analysis of genetic and aflatoxin diversity among Aspergillus flavus isolates collected from sorghum seeds

Divakara

Aiyaz

Moore

et al. 2015

J. Basic Microbiol.

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Thirty-four Aspergillus flavus isolates were recovered from sorghum seeds sampled across five states in India. Our study included (1) species confirmation through PCR assay, (2) quantification of total aflatoxin concentrations by the indirect competitive-ELISA (ic-ELISA) method, and (3) analysis of molecular diversity among the A. flavus isolates using β-tubulin, ITS, and ISSR markers. Among the isolates studied, 28 were found to be positive for the production of aflatoxins. ITS and β-tubulin phylogenetic analysis segregated the A. flavus sample population into two major groups or clades with little to no subdivision based on geography. In contrast, ISSR analysis also separated the A. flavus isolates into two main clusters, showing a distance of 0.0-0.5, with one cluster exhibiting a high level of diversity though no geographic or chemotype subdivision could be observed. The majority of sampled A. flavus isolates were highly toxigenic, and also highly diversified in terms of toxin-producing potential in-vitro. Genetic diversity among the sorghum isolates of A. flavus further warrants the development of appropriate farming management practices as well as improved aflatoxin detection measures in India.

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Molecular identification and characterization ofFusariumspp. associated with sorghum seeds

Cited by 45 publications

References 40 publications

Toxigenic molds in Tunisian and Egyptian sorghum for human consumption

Toxigenic molds in Tunisian and Egyptian sorghum for human consumption

Molecular identification and characterization of Fusarium spp. associated with wheat grains

Analysis of genetic and aflatoxin diversity among Aspergillus flavus isolates collected from sorghum seeds

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