Molecular identification and characterization of the pyruvate decarboxylase gene family associated with latex regeneration and stress response in rubber tree

Long, Xiangyu; He, Bin; Wang, Chuang; Fang, Yuxiao; Qi, Jiyan; Tang, Chaorong

doi:10.1016/j.plaphy.2014.12.005

Cited by 19 publications

(14 citation statements)

References 34 publications

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“…Based on the enriched GO terms, many DEGs in the comparison between 7 d and 0 d are presumably related to carbohydrate and energy metabolism, such as disaccharide, starch, and sucrose metabolic processes [28] (Fig. 3f).…”

Section: Discussionmentioning

confidence: 99%

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

et al. 2016

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BackgroundHickory (Carya cathayensis), a woody plant with high nutritional and economic value, is widely planted in China. Due to its long juvenile phase, grafting is a useful technique for large-scale cultivation of hickory. To reveal the molecular mechanism during the graft process, we sequenced the transcriptomes of graft union in hickory.ResultsIn our study, six RNA-seq libraries yielded a total of 83,676,860 clean short reads comprising 4.19 Gb of sequence data. A large number of differentially expressed genes (DEGs) at three time points during the graft process were identified. In detail, 777 DEGs in the 7 d vs 0 d (day after grafting) comparison were classified into 11 enriched Gene Ontology (GO) categories, and 262 DEGs in the 14 d vs 0 d comparison were classified into 15 enriched GO categories. Furthermore, an overview of the PPI network was constructed by these DEGs. In addition, 20 genes related to the auxin-and cytokinin-signaling pathways were identified, and some were validated by qRT-PCR analysis.ConclusionsOur comprehensive analysis provides basic information on the candidate genes and hormone signaling pathways involved in the graft process in hickory and other woody plants.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3182-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

et al. 2016

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“…This is consistent with the fact that mitochondria are the main compartment responsible for energy generation. Among these differentially expressed proteins, seven are carbon metabolism-related enzymes, including pyruvate decarboxylase ( Pyruvate decarboxylase (PDC) is the first and the critical enzyme of ethanol fermentation, which branches off the main glycolytic pathway at pyruvate and occurs under both anaerobic and aerobic conditions [28]. In plants, PDC involves in energy and material metabolism, responding to development and biotic and abiotic stresses, such as flowering, anoxia, cold, salt, wounding, and pathogen infection [29].…”

Section: Proteins Involved In Carbohydrate Metabolism and Energy Prodmentioning

confidence: 99%

Proteomic analysis of changes in mitochondrial protein expression during peach fruit ripening and senescence

Jiang

et al. 2016

Journal of Proteomics

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“…During latex regeneration, the PPP supplies NADPH for the reducing reactions in rubber biosynthesis, and supplies pentose for nucleic acid synthesis ( Lynen, 1969 ). Various environmental factors such as cold, wind, disease, and especially wounding stresses from tapping affect rubber yield ( Sookmark et al, 2002 ; Venkatachalam et al, 2009 ; Li et al, 2010 ; Gebelin et al, 2013 ; Long et al, 2015 ). The act of tapping the tree inevitably imposes a form of wounding stress that leads to peroxide accumulation in latex.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of the Glucose-6-Phosphate Dehydrogenase Gene Family in the Para Rubber Tree, Hevea brasiliensis

Long

Fang

et al. 2016

Front. Plant Sci.

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As a key enzyme in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides nicotinamide adenine dinucleotide phosphate (NADPH) and intermediary metabolites for rubber biosynthesis, and plays an important role in plant development and stress responses. In this study, four Hevea brasiliensis (Para rubber tree) G6PDH genes (HbG6PDH1 to 4) were identified and cloned using a genome-wide scanning approach. All four HbG6PDH genes encode functional G6PDH enzymes as shown by heterologous expression in E. coli. Phylogeny analysis and subcellular localization prediction show that HbG6PDH3 is a cytosolic isoform, while the other three genes (HbG6PDH1, 2 and 4) are plastidic isoforms. The subcellular locations of HbG6PDH3 and 4, two latex-abundant isoforms were further verified by transient expression in rice protoplasts. Enzyme activity assay and expression analysis showed HbG6PDH3 and 4 were implicated in PPP during latex regeneration, and to influence rubber production positively in rubber tree. The cytosolic HbG6PDH3 is a predominant isoform in latex, implying a principal role for this isoform in controlling carbon flow and NADPH production in the PPP during latex regeneration. The expression pattern of plastidic HbG6PDH4 correlates well with the degree of tapping panel dryness, a physiological disorder that stops the flow of latex from affected rubber trees. In addition, the four HbG6PDHs responded to temperature and drought stresses in root, bark, and leaves, implicating their roles in maintaining redox balance and defending against oxidative stress.

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Molecular identification and characterization of the pyruvate decarboxylase gene family associated with latex regeneration and stress response in rubber tree

Cited by 19 publications

References 34 publications

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process

Proteomic analysis of changes in mitochondrial protein expression during peach fruit ripening and senescence

Identification and Characterization of the Glucose-6-Phosphate Dehydrogenase Gene Family in the Para Rubber Tree, Hevea brasiliensis

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