Molecular identification and biofilm-forming ability of Elizabethkingia species

Puah, Suat Moi; Fong, Sam Pei; Kee, Boon Pin; Puthucheary, S. D.; Chua, Kek Heng

doi:10.1016/j.micpath.2021.105345

Cited by 12 publications

(19 citation statements)

References 38 publications

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“…Identification of the capsule biosynthesis gene, capD [ 59 ], and the adeG gene for the AdeFGH efflux pump [ 20 ] in all Elizabethkingia species ( Supplementary Table S2 ) leads to possible biofilm formation [ 44 , 63 ], which empowers the bacteria with the ability to persist on various surfaces [ 59 , 64 ]. Thirty clinical isolates from Malaysia, which comprised E. anophelis , E. meningoseptica, and E. miricola, were recently shown to produce biofilms on polystyrene microtiter plates [ 65 ].…”

Section: Virulence Factorsmentioning

confidence: 99%

“…Although there are currently no published reports on E. anophelis infections in Malaysia, whole-genome sequencing and assembly of a clinical isolate of E. anophelis B2D had been submitted to NCBI (Accession: PRJNA248328) by the University of Malaya but as E. meningoseptica . This led a group of scientists from the University of Malaya to revive thirty archived lyophilized isolates collected from their University Hospital that were initially identified as Flavobacterium meningosepticum [ 65 ]. Re-identification using 16S rRNA sequencing revealed that 24 were actually E. anophelis, whereas the remaining six were E. miricola [ 65 ].…”

Section: Malaysia Reportsmentioning

confidence: 99%

“…This led a group of scientists from the University of Malaya to revive thirty archived lyophilized isolates collected from their University Hospital that were initially identified as Flavobacterium meningosepticum [ 65 ]. Re-identification using 16S rRNA sequencing revealed that 24 were actually E. anophelis, whereas the remaining six were E. miricola [ 65 ]. None of the isolates were identified as E. meningoseptica , underlining the very high possibility of misidentification of these pathogens from earlier publications, particularly those that relied on traditional biochemical tests for their identification.…”

Section: Malaysia Reportsmentioning

confidence: 99%

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Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

2022

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Elizabethkingia spp. is a ubiquitous pathogenic bacterium that has been identified as the causal agent for a variety of conditions such as meningitis, pneumonia, necrotizing fasciitis, endophthalmitis, and sepsis and is emerging as a global threat including in Southeast Asia. Elizabethkingia infections tend to be associated with high mortality rates (18.2–41%) and are mostly observed in neonates and immunocompromised patients. Difficulties in precisely identifying Elizabethkingia at the species level by traditional methods have hampered our understanding of this genus in human infections. In Southeast Asian countries, hospital outbreaks have usually been ascribed to E. meningoseptica, whereas in Singapore, E. anophelis was reported as the main Elizabethkingia spp. associated with hospital settings. Misidentification of Elizabethkingia spp. could, however, underestimate the number of cases attributed to the bacterium, as precise identification requires tools such as MALDI-TOF MS, and particularly whole-genome sequencing, which are not available in most hospital laboratories. Elizabethkingia spp. has an unusual antibiotic resistance pattern for a Gram-negative bacterium with a limited number of horizontal gene transfers, which suggests an intrinsic origin for its multidrug resistance. Efforts to prevent and further understand Elizabethkingia spp. infections and limit its spread must rise to this new challenge.

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Section: Virulence Factorsmentioning

confidence: 99%

Section: Malaysia Reportsmentioning

confidence: 99%

Section: Malaysia Reportsmentioning

confidence: 99%

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Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

2022

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“…The potential of E. meningoseptica to produce biofilm indicates that it has the ability to impede treatment. Numerous investigations involving E. meningoseptica infection or outbreaks caused by flume or faucet drainage pollution have demonstrated that once biofilm is developed, it is difficult to eliminate from the environment ( 5 , 20 , 45 ).…”

Section: Discussionmentioning

confidence: 99%

Epidemiological, clinical, and laboratory features of patients infected with Elizabethkingia meningoseptica at a tertiary hospital in Hefei City, China

Liu²,

Shi³

et al. 2022

Front. Public Health

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BackgroundElizabethkingia meningoseptica is a bacterium causing potential nosocomial infections and is associated with a high mortality rate; however, the date of patients in the Hefei population who have been diagnosed with this infection is generally limited.PurposeThe clinical and laboratory data of patients from a tertiary hospital in Hefei City who had E. meningoseptica infection were evaluated in this retrospective analysis.Patients and methodsFrom May 2017 to November 2021, there were 24 patients infected with E. meningoseptica in the First Affiliated Hospital of Anhui Medical University. Data were gathered from the hospital's electronic medical records for all patients.ResultsThe most prevalent symptom among the 24 patients was fever (83.3%), followed by edema (41.7%), cough (37.5%), altered consciousness (41.7%), and sputum (37.5%), and laboratory results presented with anemia (75%), hypoproteinemia (75%), elevated C-reactive protein (CRP) (66.7%), neutrophilia (54.2%), and leukocytosis (50.0%). Hepatic disease (1 vs. 7, P = 0.009) was the only significant risk factor for underlying diseases. The mean value of lymphocyte (LYMPH#) (1.4 vs. 0.83 × 109/L, P = 0.033) counts was higher in the survival group than death group, while both anemia (8 vs. 10, P = 0.024) and hypoproteinemia (8 vs. 10, P = 0.024) occurred more frequently in the death group compared with the survival one.ConclusionFever was the most common symptom and the only significant factor of underlying diseases was hepatic disease (P = 0.009) that often occurred in death groups. In this investigation, the risk factors for death in patients were anemia, hypoproteinemia, and lymphocyte count. The susceptibility of some quinolones, piperacillin-tazobactam, and cotrimoxazole was relatively high, suggesting that they may be the preferred drugs for the treatment of E. meningoseptica infection. As E. meningoseptica can produce biofilm to pollute the hospital environment and cause infection in patients, the disinfection of the hospital environment should be strengthened and medical staff should pay attention to aseptic operations.

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“…Finally in 2005, these species were reclassified into a new genus, Elizabethkingia , based on 16S rRNA gene sequencing. 3,16,18 The third pathogenic species, E anopheles , was isolated from the midgut of the Anopheles gambiae mosquito in Gambia, Africa in 2011; 6,14,18,22 however, no causal link has been established between the mosquito as a vector and transmission of E anophelis to humans. 6…”

Section: Discussionmentioning

confidence: 99%

Septicemia caused by an emerging pathogen, Elizabethkingia miricola, in a laboratory colony of African dwarf frogs (Hymenochirus curtipes)

Yang

Mani

et al. 2023

Vet Pathol

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An outbreak of morbidity and mortality in an African dwarf frog ( Hymenochirus curtipes) colony was reported following arrival at an animal research facility. Animals were found dead on arrival or became moribund shortly thereafter, and additional animals showed clinical signs of lethargy, weight loss, and anorexia over the following 3 weeks. Externally, some affected animals presented with multifocal areas of hyperemia in the inguinal and axillary areas and on the limbs, and mottled tan discoloration along the ventral abdomen. Histologically, lesions were consistent with generalized septicemia, characterized by granulomatous meningitis, otitis media, peritonitis (coelomitis), myocarditis and pericarditis, nephritis, pneumonia, and arthritis. Gram staining identified gram-negative rod-shaped bacteria free within tissues and within macrophages. Culture results of coelomic swabs identified moderate to numerous Elizabethkingia miricola. Testing of water from tanks housing affected animals showed elevated levels of nitrites and ammonia, and the presence of Citrobacter, Aeromonas, Pseudomonas, and Staphylococcus spp. cultured from several tank biofilters. E miricola is a newly recognized and rapidly emerging opportunistic pathogen in anurans and has been reported as a cause of septicemia in humans. This report documents the first occurrence of E. miricola septicemia in African dwarf frogs and illustrates the importance of this potential pathogen in the laboratory setting for amphibian research colonies, as well as those individuals directly working with them.

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Molecular identification and biofilm-forming ability of Elizabethkingia species

Cited by 12 publications

References 38 publications

Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

Epidemiology and Characteristics of Elizabethkingia spp. Infections in Southeast Asia

Epidemiological, clinical, and laboratory features of patients infected with Elizabethkingia meningoseptica at a tertiary hospital in Hefei City, China

Septicemia caused by an emerging pathogen, Elizabethkingia miricola, in a laboratory colony of African dwarf frogs (Hymenochirus curtipes)

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