Molecular hydrogen exposure improves functional state of red blood cells in the early postoperative period: a randomized clinical study

Abstract

Molecular hydrogen (H 2 ) has been considered a preventive and therapeutic medical gas in numerous diseases. The study aimed to investigate the potential role of molecular hydrogen as a component of anesthesia in surgical treatment with cardiopulmonary bypass (CPB) of acquired valve defects on the functional state of red blood cells (RBC) and functional indicators of cardiac activity. This clinical trial was conducted with 20 patients referring to the Specialized Cardiosurgical Clinical Hospital, Nizhny Novgorod, Russian Federation, who underwent elective surgery with CPB. Twenty-four patients were randomly assigned to two groups. First group included 12 patients (research group) who received H 2 at a concentration of 1.5–2.0% through a facemask using a breathing circuit of the ventilator together with anesthesia immediately after tracheal intubation and throughout the operation. Second group (control group) included 12 patients who were not given H 2 . Blood samples were withdrawn from peripheral veins and radial artery at four stages: immediately after the introduction of anesthesia (stage 1), before the start of CPB (stage 2), immediately after its termination (stage 3) and 24 hours after the operation (the early postoperative period) (stage 4). An increase in electrophoretic mobility, an increase in the metabolism of red blood cells, and a decrease in the aggregation of red blood cells relative to the corresponding indicators of the control group were observed in the research group. Patients in the research group had a decrease in oxidative stress manifestations most pronounced one day after the operation. There was a statistically significant difference between the indicators of myocardial contractile function in the research and control group on the 1 st and 3 rd days after surgery. H 2 inhalation leads to improvement of functional state of red blood cells, which is accompanied by a more favorable course of the early postoperative period. These data show the presence of protective properties of molecular hydrogen.

Therapeutic Inhalation of Hydrogen Gas for Alzheimer’s Disease Patients and Subsequent Long-Term Follow-Up as a Disease-Modifying Treatment: An Open Label Pilot Study

“…Recently, a number of randomized clinical studies with inhalation of H 2 gas have been conducted in a variety of diseases. For example, H 2 inhalation improved physical and respiratory function in acute post-COVID-19 patients [ 30 ]; a breathlessness, cough, and sputum scale score in patient with acute exacerbation of chronic obstructive pulmonary disease [ 31 ]; functional state of red blood cells, which is accompanied by a more favorable course of the early postoperative period [ 32 ]; and improved systemic inflammation and liver histology in patients with moderate-severe non-alcoholic fatty liver disease [ 33 ].…”

The Therapeutic Potential of Oxyhydrogen Gas in Oncology: A Study on Epstein–Barr Virus-Immortalised B-Lymphoblastoid (TK6) Cells

“…To date, a rapidly increasing amount of research into the physiological effects of consuming hydrogen gas (H 2 ) has identified anti-allergy, anti-inflammatory and antioxidant potentials, which may be relevant for the treatment of numerous human-related diseases and disorders [1][2][3][4][22][23][24]. To assess whether oxyhydrogen would affect the cell viability and proliferation of immortalised immune cells (TK6-B-lymphocytes), this novel enquiry focused on the effects of infusing oxyhydrogen gas into cell culture media in an attempt to simulate the most likely dispersal and retention patterns of the aforementioned gas in the blood or serous fluids.…”

“…There is an increasing body of evidence that both molecular hydrogen (H 2 ) and oxyhydrogen therapies are emerging as effective anti-inflammatory and antioxidant gases [1][2][3][4], which are physiologically well tolerated, as demonstrated in a comprehensive range of clinical trials [5][6][7][8][9].…”

Effects of Molecular Hydrogen in the Pathophysiology and Management of Cardiovascular and Metabolic Diseases

“…These findings indicate that may protect vascular tissues from damage induced by shear stress and hydroxyl radicals, while preserving the beneficial effects of nitric oxide on vasomotor function. Given that oxidative stress can exacerbate systemic inflammation and thereby impair the function of cardiomyocytes, beta cells, and neurons, as well as endothelium, the potential protective roles of warrant further investigation [ 48 , 49 , 50 , 51 , 52 ].…”